Characterization of phenolic compounds in the fruits of Forsythia suspensa by high‐performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Köse

et al. 2015

Bangladesh J Pharmacol

The aim of this study was to reveal the phytochemical constituents, antioxidant and antimicrobial activity of Ajuga orientalis. According to the antimicrobial results, the methanol extract of A. orientalis showed a MIC value of 312.5 µg/mL against the tested pathogenic bacterial strains. Anticandidal activity of extract was found as 156.3 µg/mL both against Candida albicans and C. parapsilosis strains. Whereas the extract was more effective against C. tropicalis with the MIC value of 78.1 µg/mL. The in vitro DPPH radical scavenging activity of the extract was determined as IC50=0.4 ± 0.02 mg/mL whereas the standard BHT IC50 was 0.01 ± 0.00 mg/mL. Trolox Equivalent Antioxidant Capacity (TEAC) of extract was determined 1.3 mM TEAC, while BHT, 1.9 mM TEAC.

Section: Resultssupporting

confidence: 60%

Phytochemical characterization of phenolics by LC-MS/MS and biological evaluation of Ajuga orientalis from Turkey

Köse

et al. 2015

Bangladesh J Pharmacol

“…Ions observed in the MS 2 analysis of the deprotonated molecule showed typical fragmentation pathways with a methoxyl group substituted in the b position. [30] With reference to its demethoxyl analogue, and to the absence of other kinds of phenylethanoid glycosides, compound 53 was tentatively identified as b-methoxylverbascoside.…”

Section: Secoiridoidsmentioning

confidence: 99%

“…This dilignol was the major component in the acetone extract from thermally modified F. excelsior wood, [15] after 4 h at 200 C in a nitrogen atmosphere and under pressure. It can therefore be considered thermally very stable, which facilitate its use as a food additive, a nutraceutical or a drug, since these families of compounds have been the subject of many papers in recent years, [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] due to their important biological activity. [39] Unfortunately, we found very little data in regard to their response to thermal treatments that could be used in the food or pharmaceutical industry.…”

Section: Dilignols and Oligolignolsmentioning

confidence: 99%

LC‐DAD/ESI‐MS/MS study of phenolic compounds in ash (Fraxinus excelsior L. and F. americana L.) heartwood. Effect of toasting intensity at cooperage

Sanz¹,

Simón

Cadahía

et al. 2012

J. Mass. Spectrom.

The phenolic composition of heartwood extracts from Fraxinus excelsior L. and F. americana L., both before and after toasting in cooperage, was studied using LC-DAD/ESI-MS/MS. Low-molecular weight (LMW) phenolic compounds, secoiridoids, phenylethanoid glycosides, dilignols and oligolignols compounds were detected, and 48 were identified, or tentatively characterized, on the basis of their retention time, UV/Vis and MS spectra, and MS fragmentation patterns. Some LMW phenolic compounds like protocatechuic acid and aldehyde, hydroxytyrosol and tyrosol, were unlike to those for oak wood, while ellagic and gallic acid were not found. The toasting of wood resulted in a progressive increase in lignin degradation products with regard to toasting intensity. The levels of some of these compounds in medium-toasted ash woods were much higher than those normally detected in toasted oak, highlighting vanillin levels, thus a more pronounced vanilla character can be expected when using toasted ash wood in the aging wines. Moreover, in seasoned wood, we found a great variety of phenolic compounds which had not been found in oak wood, especially oleuropein, ligstroside and olivil, along with verbascoside and isoverbascoside in F. excelsior, and oleoside in F. americana. Toasting mainly provoked their degradation, thus in medium-toasted wood, only four of them were detected. This resulted in a minor differentiation between toasted ash and oak woods. The absence of tannins in ash wood, which are very important in oak wood, is another peculiar characteristic that should be taken into account when considering its use in cooperage.

“…It provides a powerful approach to characterize and identify a variety of polar and thermally labile compounds such as flavonoids in crude extracts of plants (Wang et al 2004;Schutz and others 2005;Tai et al 2006;Guo et al 2007;Ren et al 2007;Zheng et al 2007;Avul et al 2008;Liu et al 2008). Various hyphenated and hybrid mass spectrometers have now become widely adopted, and, among them, TimeOf-Flight (TOF) MS and its hybrid or in combination use with Tandem Mass Spectrometry (MS/MS) have proven to be very powerful tools for the structural characterization of natural compounds in view of their complementary capacity to provide multistage fragmentations and accurate mass measurements for precise elemental compositions of molecular ions and subsequent product ions (Schutz et al 2005;Ferrer et al 2004;Yokosuka et al 2006;Zhu et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Rapid Identification and Detection of Flavonoids Compounds From Bamboo Leaves by Lc-(Esi)-It-Tof/MS

Lv¹,

Dong²,

Zhang³

2012

BioResources

This paper provides an applicable approach to identifying flavonoid compounds from bamboo leaves extracts, based on the use of the powerful Liquid Chromatography Electrospray Ionization source in combination with hybrid Ion Trap and high-resolution Time-of-flight Mass Spectrometry (LC-(ESI)-IT-TOF/MS). The strategy involves four procedural steps including searching flavonoid components based on an ultraviolet spectrum scan, getting the accurate mass of flavonoid components parent ion, retrieving the corresponding formula by software, and speculating as to the chemical structure according to mass spectrum decomposition rules. The presently developed methodology has been well proven to be useful and valuable by successful application to the identification of flavonoid components from Dendrocalamopsis oldham leaves. All of the 13 flavonoid components detected have been successfully identified by this approach, except that it failed to confirm 3 flavonoid component chemical structures. The calibration curves of two flavonoid components (orientin and vitexin) that had been identified in bamboo leaves showed a good linear fit (R 2 ≥ 0.9998) in the concentration range of 6.25 to 200 mg/L. The limits of detection (LOD) were less 0.02 mg/L (S/N=3), and the estimated limits of quantification (LOQ) were less 0.06 mg/L (S/N=10). Intra-and inter-day relative standard deviations were less than 1.04 and 1.82%, respectively.