Phytochemical characterization of phenolics by LC-MS/MS and biological evaluation of &lt;i&gt;Ajuga orientalis&lt;/i&gt; from Turkey

Göğer, Fatih; Köse, Yavuz Bülent; Göğer, Gamze; Demirci, Fatih

doi:10.3329/bjp.v10i3.23500

Cited by 21 publications

(11 citation statements)

References 6 publications

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“…Forsythoside A is rarely found in the Lamiceae family. Although it has been described to occur in Teucrium [ 17 ] and Ajuga plants [ 26 ]. This is the first report of its presence in the genus Salvia .…”

Section: Resultsmentioning

confidence: 99%

Determination of the Phenolic Profile and Antioxidant Properties of Salvia viridis L. Shoots: A Comparison of Aqueous and Hydroethanolic Extracts

Grzegorczyk-Karolak

Kiss

2018

Molecules

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Salvia viridis L. is an annual herb used in Mediterranean medicine. The purpose of this study was to determine the polyphenol profile of aqueous (decoction and infusion) and hydroethanolic extracts of aerial parts of field-grown S. viridis and to evaluate their antioxidant activity. The polyphenol profiling was performed via UPLC-DAD/ESI-MS. Additionally, the total polyphenol content in extracts tested were determined by UV-Vis spectrophotometry using the Folin-Ciocalteu assay. The antioxidant effect was evaluated by the FRAP, DPPH, ABTS, O2•− scavenging and TBARS methods. The hydroethanolic extract gave the highest content of total phenolic compounds, followed by the infusion. The UPLC-DAD/ESI-MS analysis of extracts showed a total of 19 phenolic compounds identified as flavonoids (four compounds), phenylethanoids (eight compounds) and phenolic acids (seven compounds). Rosmarinic acid was the predominant phenolic acid, verbascoside was the predominant phenylethanoid, while apigenin glucuronide or methylluteolin glucuronide, depending on the sample, were the predominant flavonoids in the analyzed extracts. The presence of a high polyphenol level indicated a high antioxidant activity of both the infusion and the hydroalcoholic extract. These results indicate that S. viridis is a rich resource of phenolic compounds and can be used in dietary applications with the potential to reduce oxidative stress.

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Section: Resultsmentioning

confidence: 99%

Determination of the Phenolic Profile and Antioxidant Properties of Salvia viridis L. Shoots: A Comparison of Aqueous and Hydroethanolic Extracts

Grzegorczyk-Karolak

Kiss

2018

Molecules

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“…For LC-MS/MS analysis, we used the method described by Goger et al (43) with some modifications. The experiments were performed with a Shimadzu 20A highperformance liquid chromatography (HPLC) system coupled to an Applied Biosystems 3200 Q-Trap LC-MS/ MS instrument (Thermo Fisher Scientific, Waltham, MA, USA) equipped with an electrospray ionisation (ESI) ion source in negative ionisation mode.…”

Section: Determination Of Phenolic Compounds In Em1 and Em2 Extractsmentioning

confidence: 99%

Chemical composition and antioxidant, cytotoxic, and insecticidal potential of Valeriana alliariifolia in Turkey

Sen-Utsukarci

Taşkın

Göğer

et al. 2019

Archives of Industrial Hygiene and Toxicology

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Valeriana is a common plant species used for various healing purposes in folk medicine since antiquity. This study investigates the phytochemical profile, antioxidant, cytotoxic, and insecticidal activity of Valeriana alliariifolia Adams, a species that has traditionally been used in Turkey. For the analyses we prepared four root extracts of V. alliariifolia Adams using hexane (HM1), chloroform (CM1), ethanol (EM1), and water (WM1) for maceration. Additionally, two extracts were also prepared from its roots by maceration separately with ethanol (EM2) and water (WM2). One sample was prepared as a water infusion (WI), according to the procedure used in Turkish traditional medicine. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation scavenging activity tests showed that ethanol extracts had the strongest antioxidant activity: EM1 (IC50 – DPPH: 17.694 µg/mL; ABTS: 23.8 µg/mL) and EM2 (IC50 – DPPH: 20 µg/mL; ABTS: 21.5 µg/mL). The hexane extract, HM1, was the most cytotoxic (IC50<10 µg/mL against HepG2 and HUVEC) and EM2 strongly cytotoxic (IC50<10 µg/mL against HepG2 and IC50: 11.96 µg/mL against HUVEC). The extracts with demonstrated cytotoxic activities were further examined to check their insecticidal activity against adult female mosquito Aedes aegypti and first instar Ae. aegypti larvae. HM1 was the most effective (90±10 %), which was consistent with its cytotoxic activity. Because of the high antioxidant, cytotoxic, and insecticidal activities, we ran phytochemical analyses of the HM1, EM1, and EM2 extracts with GC-MS (for HM1) and LC-MS/MS (for EM1 and EM2). We also analysed the composition of the essential oil obtained from V. alliariifolia roots by micro-distillation in order to compare its content with HM1, which contains volatile compounds. Phytochemical analyses revealed that the major compound in HM1 was isovaleric acid (16 %) and in the essential oil 1,8-cineole (2.9 %). EM1 and EM2 contained 5-O-caffeoylquinic acid (chlorogenic acid), verbascoside (acteoside), and 3,5-dicaffeoylquinic acid as major components. In the light of our findings and available literature, we can conclude that V. alliariifolia has a good bioactive potential that could be used for different purposes, including the development of new agents for the treatment of various diseases. The difference in the content between the essential oil and HM1 was remarkable. It suggests that the variability observed in the activity of the samples was a result of composition and that, therefore, the aim of treatment should dictate which type of preparation is to be selected. An added value of our study is that it determined verbascoside and methylquercetin rutinoside for the first time in the Valeriana extracts.

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“…The results showed (Figure 3) that the highest total polyphenols content (TPC) was recorded in the flowers sample 1 (sample FL1), which yielded the result 5.706 ± 0.8992 g (expressed as tannic acid equivalents)/100 g dry herbal product, while, on the other hand, the smallest amount was recorded in the roots (sample R4), namely 1.0108 ± 0.2742 g (expressed as tannic acid equivalents)/100 g dry herbal product. [7]. However, a comparison with data in scientific literature cannot be made because our results are expressed in tannic acid equivalents.…”

Section: Quantitative Analysis Of Total Polyphenols (Tpc)mentioning

confidence: 98%

Evaluation of the Chemical Composition of Ajuga Chamaepitys (L.) Schreb. From the Spontaneous Flora of Romania

Ionus¹

2021

FARMACIA

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Ajuga chamaepitys (L.) Schreb., traditionally named yellow bugle, is a perennial species that is part of the Dobrogea region, Romania, spontaneous flora, associated in traditional phytotherapy. The aim of this study was to determine the qualitative and quantitative chemical composition of different types of plant organs (aerial parts, leaves, flowers, stems and roots), as well as the optimal harvesting interval. Thus, higher levels in the flavones, phenolic acids and total polyphenols content were recorded in the plant's flowers, our analysis yielding 7.1501 g flavonoid content (expressed as rutin equivalents)/100 g dry herbal product, 1.5751 g phenolic acids content (expressed as chlorogenic acid equivalents)/100 g dry herbal product, respectively 5.706 g total phenolic content (expressed as tannic acid equivalents)/100 g dry herbal product. RezumatAjuga chamaepitys (L.) Schreb., tămâiță de câmp, este o specie perenă întâlnită în flora spontană din regiunea Dobrogei, România, asociată în fitoterapia tradițională. Scopul studiului a fost determinarea compoziției chimice calitative și cantitative a diferitelor tipuri de organe vegetale (părți aeriene, frunze, flori, tulpini și rădăcini), precum și a intervalului optim de recoltare al acestora. Conţinutul maxim în flavone, acizi fenolici şi polifenoli totali a fost înregistrat în probele de flori, obţinându-se 7,1501 g flavone (exprimați în echivalenți de rutin)/100 g produs vegetal uscat, 1,5751 g acizi fenolici (exprimați în echivalenți de acid clorogenic)/100 g produs vegetal uscat, respectiv 5,706 g polifenoli totali (exprimaţi în echivalenți de acid tanic)/100 g produs vegetal uscat.

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Phytochemical characterization of phenolics by LC-MS/MS and biological evaluation of <i>Ajuga orientalis</i> from Turkey

Cited by 21 publications

References 6 publications

Determination of the Phenolic Profile and Antioxidant Properties of Salvia viridis L. Shoots: A Comparison of Aqueous and Hydroethanolic Extracts

Determination of the Phenolic Profile and Antioxidant Properties of Salvia viridis L. Shoots: A Comparison of Aqueous and Hydroethanolic Extracts

Chemical composition and antioxidant, cytotoxic, and insecticidal potential of Valeriana alliariifolia in Turkey

Evaluation of the Chemical Composition of Ajuga Chamaepitys (L.) Schreb. From the Spontaneous Flora of Romania

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