Characterization of paromomycin sulfate by capillary electrophoresis with UV detection after pre-capillary derivatization

Abstract

SummaryA capillary electrophoretic method has been developed for determination of the components of the paromomycin mixture salt. Paromomycin was detected at 330 nm after pre-capillary derivatization with o-phthaldialdehyde and thioglycohc acid. The electrophoretic separation was performed in a fused-silica capillary at 25 ~ and 18 kV with a background electrolyte comprising 40 mM sodium tetraborate, 3 mM fl-cyclodextrin, and 12.5% (v/v) methanol.Although the analysis time was reduced to 10 min, five peaks could be separated to baseline. The relative standard deviation of the ratios (peak area/internal standard peak area) was <5% for all peaks.

A new micellar electrokinetic capillary chromatography method for separation of the components of the aminoglycoside antibiotics

“…However, Since all aminoglycoside antibiotics are lacking of a chromophore or fluorophore derivatization is essential. Beside other derivatization agents such as 2,4-dinitrofluorobenzene [9][10][11], dansyl chloride [12], 9-fluorenylmethyl chloroformiate [13,14], or 1-methoxycarbonylindolizine-3,5-dicarbaldehyde, o-phthaldialdehyde (OPA) in the presence of thioglycolic acid (TGA) has turned out to be advantageous for all aminoglycosides [15][16][17]. CE in combination with a precapillary derivatization with the OPA/TGA system was found to be appropriate for separation and quantification of the components of the aminoglycosides and the impurities when using a background electrolyte (BGE) composed of tetraborate, b-cyclodextrin (b-CD) and methanol [7,18,19].…”

Analysis of antibiotics by capillary electrophoresis

“…The method was linear for tobramycin from 0.01 to 1.75 mg/mL, and was applied to the analysis of five commercial samples of tobramycin [4]. Another aminoglycoside, paromomycin, was derivatized with OPA and thioglycollic acid prior to separation [5]. Using a 40 mM borate buffer containing 3 mM b-cyclodextrin (b-CD) and 12.5% v/v MeOH, five peaks from this complex were baselineseparated within 10 min [5].…”

“…Another aminoglycoside, paromomycin, was derivatized with OPA and thioglycollic acid prior to separation [5]. Using a 40 mM borate buffer containing 3 mM b-cyclodextrin (b-CD) and 12.5% v/v MeOH, five peaks from this complex were baselineseparated within 10 min [5].…”

Direct determination of gentamicin components by capillary electrophoresis with potential gradient detection

“…Among them, HPCE, due to its high separation efficiency and small usage of reagents, has become an attractive technique to determine the different components of gentamicin. Normally derivatization is required to detect gentamicin because of its lack of UV chromatophore [13][14][15][16][17]. Postcolumn or precolumn derivatizations by 1,2-phthalic dicarboxaldehyde (OPA), dansylchloride and mercaptoacetic acid (MAA) with either UV or fluorescence detection have been reported [13][14][15][16][17].…”

“…To measure the relative percentages of the major components, several other methods have been developed to separate gentamicin, such as thin-layer chromatography (TLC) [4], ion-exchange chromatography [5], high-performance liquid chromatography (HPLC) [6][7][8][9][10][11][12], and highperformance capillary electrophoresis (HPCE) [13][14][15][16][17][18][19][20]. Among them, HPCE, due to its high separation efficiency and small usage of reagents, has become an attractive technique to determine the different components of gentamicin.…”