Characterization of osteoclast precursors in human blood

Shalhoub, Victoria; Elliott, Gary; Chiu, Laura; Manoukian, Raffi; Kelley, M.; Hawkins, Nessa; Davy, Elyse; Shimamoto, Grant; Beck, J.; Sl, Kaufman; Van, Gwyneth; Scully, Sheila; Qi, Ming; Grisanti, Mario; Dunstan, Colin R.; Boyle, W. J.; Lacey, David L.

doi:10.1046/j.1365-2141.2000.02379.x

Cited by 112 publications

(44 citation statements)

References 33 publications

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“…The number and diversity of tissues that express TRANCE imply actions beyond those described in lymphoid and bone biology. Moreover, monocytes express RANK (53). What is the effect of extraosseous TRANCE on these cells?…”

Section: Discussionmentioning

confidence: 99%

TGF-β1 and IFN-γ Direct Macrophage Activation by TNF-α to Osteoclastic or Cytocidal Phenotype

Fox

Fuller

Bayley

et al. 2000

The Journal of Immunology

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TNF-related activation-induced cytokine (TRANCE; also called receptor activator of NF-κB ligand (RANKL), osteoclast differentiation factor (ODF), osteoprotegerin ligand (OPGL), and TNFSF11) induces the differentiation of progenitors of the mononuclear phagocyte lineage into osteoclasts in the presence of M-CSF. Surprisingly, in view of its potent ability to induce inflammation and activate macrophage cytocidal function, TNF-α has also been found to induce osteoclast-like cells in vitro under similar conditions. This raises questions concerning both the nature of osteoclasts and the mechanism of lineage choice in mononuclear phagocytes. We found that, as with TRANCE, the macrophage deactivator TGF-β1 strongly promoted TNF-α-induced osteoclast-like cell formation from immature bone marrow macrophages. This was abolished by IFN-γ. However, TRANCE did not share the ability of TNF-α to activate NO production or heighten respiratory burst potential by macrophages, or induce inflammation on s.c. injection into mice. This suggests that TGF-β1 promotes osteoclast formation not only by inhibiting cytocidal behavior, but also by actively directing TNF-α activation of precursors toward osteoclasts. The osteoclast appears to be an equivalent, alternative destiny for precursors to that of cytocidal macrophage, and may represent an activated variant of scavenger macrophage.

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Section: Discussionmentioning

confidence: 99%

TGF-β1 and IFN-γ Direct Macrophage Activation by TNF-α to Osteoclastic or Cytocidal Phenotype

Fox

Fuller

Bayley

et al. 2000

The Journal of Immunology

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“…We screened the chemokine gene expression in benign osteolytic bone tumors in order to identify the candidate molecules responsible for the recruitment of osteoclast precursors into the area of bone destruction by tumor osteoclasts. Among these cheniokines CXCR4, unique receptor of SDF-I, is known to be expressed in monocytes and hematopoietic stem cells [2,6] that can serve as osteoclast precursor cells [27]. Since all of the benign osteolytic lesions consisted mainly of stromal cells, it would be quite valuable to determine the role of the SDF-1 that these neoplastic stromal cells produce during osteoclastogenesis.…”

Section: Introductionmentioning

confidence: 99%

Recruitment of osteoclast precursors by stromal cell derived factor‐1 (SDF‐1) in giant cell tumor of bone

Liao

Yurgelun

et al. 2005

Journal Orthopaedic Research

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Giant cell tumor (GCT) of bone is a unique bone lesion that is characterized by an excessive number of multinucleated osteoclasts. GCT consists of neoplastic stromal cells, multinucleated osteoclasts and their precursors, thus serving as a naturally occurring human disease model for the study of osteoclastogenesis. It still remains unclear how stromal cells of GCT recruit osteoclast precursors. In the present study, we characterized the cellular components of GCT and confirmed the presence of C D 14+-monocytes/CD68'-macrophages and CD34+-heniatopoetic stem cells that express CXCR4, a specific receptor for SDF-1; SDF-1 gene expression and presence of SDF-1 protein were confirmed by real time RT-PCR, in situ hybridization, and immunohistochemistry in the GCT tissue and cultured cells. SDF-1 was present at 25-50ng/ml in the conditioned media from the GCT cultures, which is in the range of physiological chemotactic concentration. Migration of osteoclast precursors was 2.5-fold higher in response to GCT conditioned media compared to the control media; and migration was inhibited by an average of 36% with anti-SDF-1 neutralizing antibody or competing recombinant SDF-1. These results suggest that SDF-1 is one of the significant chemoattractant factors involved in the recruitment of hematopoietic osteoclast precursor cells during tumor-induced osteoclastogenesis.

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“…2c) and morphology (multiple nuclei) of osteoclasts were also evident in fairly large numbers. TRAP positive osteoclasts, in their precursor stage are found in the PB among the monocytic population (Shalhoub et al 2000) but, although the expression of TRAP is regarded as an osteoclast marker, under certain conditions, cells of the macrophage/dendritic cell family also express TRAP (Tsuboi et al 2003). FACS analysis further fortified the monocyte/macrophage identity of the primary adherent cells which demonstrated 90% positive for the leukocyte-common antigen CD45 and negative for CD34 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Isolation of progenitor cells from cord blood using adhesion matrices

2007

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The aim of this study was to develop optimal conditions for selective adhesion and isolation of mesenchymal progenitor cells (MPCs) from cord blood and to determine their potential for osteogenic differentiation. Mononuclear cells (MNCs) were isolated by Ficoll-Paque gradient and plated onto 48-well culture plates precoated with: human or bovine collagen type I, human collagen type IV, fibronectin or matrigel. Cultures were incubated in aMEM containing fetal calf serum. Viability of the adherent cells was determined by alamarBlue1 assay after 2, 3, and 4 weeks. After 4 weeks in culture, cells were typsinized and replated. Primary cultures were analyzed by histochemistry and third passage cells by FACS. Isolated fibroblast-like cells were cultured in the presence of osteogenic factors and differentiation determined by Alizarin Red S staining, RT-PCR and electron dispersive spectroscopy (EDS). MNCs adhered to all types of matrices with the greatest adhesion rates on fibronectin. . Primary cultures expressed endothelial/macrophage RNA markers whether cultured on fibronectin or polystyrene and these markers decreased upon passage. The best osteogenic differentiation was observed in MPCs cultured in osteogenic medium containing vitamin D 3 and FGF9. These cells expressed the bonerelated mRNA, collagen type I, core binding factor I (Cbfa I), osteocalcin and osteopontin. EDS of deposits produced by these cells demonstrated a calcium/phosphate ratio parallel to hydroxyapatite. It was concluded that fibronectin increased adhesion rates and isolation potential of cord blood mesenchymal progenitor cells.

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Characterization of osteoclast precursors in human blood

Cited by 112 publications

References 33 publications

TGF-β1 and IFN-γ Direct Macrophage Activation by TNF-α to Osteoclastic or Cytocidal Phenotype

TGF-β1 and IFN-γ Direct Macrophage Activation by TNF-α to Osteoclastic or Cytocidal Phenotype

Recruitment of osteoclast precursors by stromal cell derived factor‐1 (SDF‐1) in giant cell tumor of bone

Isolation of progenitor cells from cord blood using adhesion matrices

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