Characterization of organosolv switchgrass lignin by using high performance liquid chromatography/high resolution tandem mass spectrometry using hydroxide-doped negative-ion mode electrospray ionization

Abstract

Method for identification of compounds in organosolv lignin.

A Fundamental Tandem Mass Spectrometry Study of the Collision‐Activated Dissociation of Small Deprotonated Molecules Related to Lignin

“…[22-29, 31, 34-36] Given that the compounds studied are representative of the compounds observed in degraded lignin mixtures [36] andc ontain similar functionalities, MS n is extremely important for identification of unknownl ignin degradation products. Hence, it is not surprising that previous studies that only utilized MS 2 or MS 3 experiments were unable to identify all of the functionalities in the analytes.…”

“…[22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] Most of these previouss tudies relied on two [23-26, 28, 29, 31, 34, 35, 38] (MS 2 )o rt hree [22,27,36] (MS 3 )s tages of mass spectrometry,t hat is, only the fragment ions of the deprotonated molecules and their fragment ions were examined. [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] Most of these previouss tudies relied on two [23-26, 28, 29, 31, 34, 35, 38] (MS 2 )o rt hree [22,27,36] (MS 3 )s tages of mass spectrometry,t hat is, only the fragment ions of the deprotonated molecules and their fragment ions were examined.…”

Identification of the Phenol Functionality in Deprotonated Monomeric and Dimeric Lignin Degradation Products via Tandem Mass Spectrometry Based on Ion–Molecule Reactions with Diethylmethoxyborane

“…Other ionization methods, such as positive ion mode ESI or atmospheric pressure chemical ionization (APCI), either cause extensive fragmentation or form multiple ion types for each compound [11]. When used in HPLC/ tandem mass spectrometry experiments, negative ion mode ESI facilitates the analysis of complex lignin degradation product mixtures [12,13]. Structural information for the ionized molecules can be obtained via multiple stages of ion isolation and collisionally activated dissociation (CAD) experiments [14].…”

“…Structural information for the ionized molecules can be obtained via multiple stages of ion isolation and collisionally activated dissociation (CAD) experiments [14]. However, isomeric ions can have similar CAD fragmentation patterns, making their differentiation challenging [12]. Therefore, new methods for structural elucidation of isomeric aromatic ions are needed.…”

Full Utilization of Lignocellulose with Ionic Liquid Polyoxometalates in a One‐Pot Three‐Step Conversion

“…[16] As Rahimi et al revealed, when the g-OH group was first selectively oxidized, the formed g-CHO end group would induce the cleavage of the C a ÀC b bond through ar etroaldolr eaction, producing aromatic aldehyde and guaiacol, in which 100 %c onversion of compound (c)w as obtained with benzylic carbonyl compounds in 82-98 %i solatedy ield upon addition of AcNH/ TEMPO/HNO 3 /HCl. 64-99 %c onversion of lignin model compoundsP P-ol and PPone to yield 45-97 %p henol,3 8-84 %a cetophenone, and 6-86 %b enzoic acid under various reactionc onditions such as 0.4-1 MPa O 2 at 80-185 8Cf or 6-48 h( Ta ble S1 in the Supporting Information).…”