Characterization of novel nonacid glycosphingolipids as biomarkers of human gastric adenocarcinoma

Jin, Chunsheng; Teneberg, Susann

doi:10.1016/j.jbc.2022.101732

Cited by 3 publications

(3 citation statements)

References 47 publications

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“…No information about the terminal Hex-Hex sequence was obtained by MS 3 . Based on the previous identification of P1 pentasaccharide ( 57 ) and considering that the α1,3-galactosyltransferase is not expressed in humans ( 58 ), we assumed that the features of the MS 2 spectrum of [M–H] – ions at m/z 868.3 allowed a tentative identification of the P1 pentasaccharide (Galα4Galβ4GlcNAcβ3Galβ4Glc).…”

Section: Resultsmentioning

confidence: 99%

“…rEGCase II from R. spp. (Takara Bio Europe S.A.) was used for the digestion of N-GSL as described ( 57 ). A detailed description of the whole procedure is listed in “ Protocol S3 ” in Supporting information.…”

Section: Methodsmentioning

confidence: 99%

“…The native N-GSL and A-GSL fractions (50 μg) dissolved in 30 μl of MeOH/MeCN (3:1, v/v) and GSL-derived oligosaccharides dissolved in 50 μl of deionized water were injected (3 μl) using a PAL HTC-xt autosampler (CTC Analytics AG) with a 2 μl sample loop. Native N-GSL and A-GSL were separated on a HILIC capillary column (100 × 0.25 mm) packed in-house with 5 μm polyamine II particles (YMC Europe GmbH), and GSL-derived neutral oligosaccharides were separated on a porous graphitized carbon capillary column (100 × 0.25 mm) packed in-house with 5 μm porous graphite particles (Hypercarb, Thermo-Hypersil) as described ( 57 ).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Comprehensive characterization of complex glycosphingolipids in human pancreatic cancer tissues

Hořejší¹,

Jin²,

Vaňková³

et al. 2023

Journal of Biological Chemistry

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive characterization of complex glycosphingolipids in human pancreatic cancer tissues

Hořejší¹,

Jin²,

Vaňková³

et al. 2023

Journal of Biological Chemistry

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Simple separation of glycosphingolipids in the lower phase of a Folch’s partition from crude lipid fractions using zirconium dioxide

Nagasawa

Miyazaki²,

Kyogashima

2022

Glycoconj J

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Galectin-4 Is Involved in the Structural Changes of Glycosphingolipid Glycans in Poorly Differentiated Gastric Cancer Cells with High Metastatic Potential

Hachisu,

Tsuchida,

Takada

et al. 2023

IJMS

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Gastric cancer with peritoneal dissemination is difficult to treat surgically, and frequently recurs and metastasizes. Currently, there is no effective treatment for this disease, and there is an urgent need to elucidate the molecular mechanisms underlying peritoneal dissemination and metastasis. Our previous study demonstrated that galectin-4 participates in the peritoneal dissemination of poorly differentiated gastric cancer cells. In this study, the glycan profiles of cell surface proteins and glycosphingolipids (GSLs) of the original (wild), galectin-4 knockout (KO), and rescue cells were investigated to understand the precise mechanisms involved in the galectin-4-mediated regulation of associated molecules, especially with respect to glycosylation. Glycan analysis of the NUGC4 wild type and galectin-4 KO clones with and without peritoneal metastasis revealed a marked structural change in the glycans of neutral GSLs, but not in N-glycan. Furthermore, mass spectrometry (MS) combined with glycosidase digestion revealed that this structural change was due to the presence of the lacto-type (β1-3Galactosyl) glycan of GSL, in addition to the neolacto-type (β1-4Galactosyl) glycan of GSL. Our results demonstrate that galectin-4 is an important regulator of glycosylation in cancer cells and galectin-4 expression affects the glycan profile of GSLs in malignant cancer cells with a high potential for peritoneal dissemination.

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Characterization of novel nonacid glycosphingolipids as biomarkers of human gastric adenocarcinoma

Cited by 3 publications

References 47 publications

Comprehensive characterization of complex glycosphingolipids in human pancreatic cancer tissues

Comprehensive characterization of complex glycosphingolipids in human pancreatic cancer tissues

Simple separation of glycosphingolipids in the lower phase of a Folch’s partition from crude lipid fractions using zirconium dioxide

Galectin-4 Is Involved in the Structural Changes of Glycosphingolipid Glycans in Poorly Differentiated Gastric Cancer Cells with High Metastatic Potential

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