Simple separation of glycosphingolipids in the lower phase of a Folch’s partition from crude lipid fractions using zirconium dioxide

Nagasawa, Hideharu; Miyazaki, Shota; Kyogashima, Mamoru

doi:10.1007/s10719-022-10080-w

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…HCOOH can be easily removed in the drying process using a centrifugal evaporator. Therefore, the throughput and cost for purification were reduced by avoiding the removal step of counter substances (such as 2,5-dihydroxybenzoic acid 13 ) using other SP columns.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we developed a procedure to separate lipids into three groups using a commercially available monolithic silica column coated with TiO 2 and zirconium dioxide (ZrO 2 ). Zr has a strong chemical affinity for phosphate groups 12 ; thus, glycolipid and phospholipid fractionation are facilitated 13 . The silica phase of the column represents electrostatic interactions with the polar moiety of lipids, helping to enrich glycolipids.…”

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confidence: 99%

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A procedure for solid phase extractions using metal oxide coated silica column in lipidomics

Takeda,

Takeuchi,

Hasegawa

et al. 2023

Preprint

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Lipid enrichment is often indispensable to enhance the coverage of targeted molecules in mass spectrometry (MS)-based lip-idomics studies. This study developed a simple stepwise fractionation method using titanium and zirconium dioxide coated solid phase (SP) silica column that separates neutral lipids, phospholipids, and other lipids including glycolipids and several polar lipids such as sulfatides. Our procedure used chloroform to dissolve lipids and the neutral lipids including cholesteryl ester, di- and triacyl glycerols were collected in the loading fraction. Secondly, methanol with formic acid (99:1, v/v) retrieved sphingolipids, glycolipids, and fatty acids by competing for affinity with Lewis acid sites on the metal oxide surface. Finally, phospholipids strongly retained via chemoaffinity interaction were eluted using a solution containing 5% ammonia and high water content (45:50 v/v, 2-propanol:water), which cancels electrostatic- and chelating interactions with the SP column. The high average reproducibility of <10% and the coverage of ~100% compared to non-SP extracts were demonstrated by untarget-ed lipidomics of human plasma, and mouse brain and feces. The advantage of our procedure was showcased by characterizing minor lipid subclasses mono- and digalactosyl monoacylglycerols in feces and acetylated- and glycolylated derivatives of gan-gliosides in brain that were not detected in conventional monophase or liquid-liquid extraction methods. Likewise, the value of our method in biology is maximized during glycolipidome profiling by the absence of phospholipids and glycolipids that cover >80% of the chromatographic peaks.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

A procedure for solid phase extractions using metal oxide coated silica column in lipidomics

Takeda,

Takeuchi,

Hasegawa

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…This is astonishing because DHB is normally much less sensitive than 9-AA [ 71 ]. Very recently, a method based on ZrO 2 binding was developed, which enabled the separation between glyco- and phospholipids [ 72 ]. This is an important step toward the detection of low-abundant glycolipids and the prevention of the suppression of these lipids by more abundant lipids.…”

Section: Which Matrix Is Most Suitable For Individual Lipid Classes?mentioning

confidence: 99%

A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids

et al. 2023

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Matrix-assisted laser desorption and ionization (MALDI) is a widely used soft-ionization technique of modern mass spectrometry (MS). MALDI enables the analysis of nearly all chemical compounds—including polar and apolar (phospho)lipids—with a minimum extent of fragmentation. MALDI has some particular advantages (such as the possibility to acquire spatially-resolved spectra) and is competitive with the simultaneously developed ESI (electrospray ionization) MS. Although there are still some methodological aspects that need to be elucidated in more detail, it is obvious that the careful selection of an appropriate matrix plays the most important role in (lipid) analysis. Some lipid classes can be detected exclusively if the optimum matrix is used, and the matrix determines the sensitivity by which a particular lipid is detected within a mixture. Since the matrix is, thus, crucial for optimum results, we provide here an update on the progress in the field since our original review in this journal in 2018. Thus, only the development during the last five years is considered, and lipids are sorted according to increasing complexity, starting with free fatty acids and ending with cardiolipins and phosphoinositides.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Simple separation of glycosphingolipids in the lower phase of a Folch’s partition from crude lipid fractions using zirconium dioxide

Cited by 4 publications

References 32 publications

A procedure for solid phase extractions using metal oxide coated silica column in lipidomics

A procedure for solid phase extractions using metal oxide coated silica column in lipidomics

A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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