Characterization of novel mutants of hexaploid wheat (<scp><i>Triticum aestivum</i></scp> L.) with various depths of purple grain color and antioxidant capacity

Hong, Min Jeong; Kim, Dae Yeon; Nam, Bo Mi; Ahn, Joon-Woo; Kwon, Soon Jae; Seo, Yong Weon; Kim, Jin Baek

doi:10.1002/jsfa.9141

Cited by 22 publications

(14 citation statements)

References 48 publications

(85 reference statements)

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“…Other desired metabolites may be enhanced through similar strategies. Anthocyanins have also been a breeding target in staple crops, such as wheat, because of their benefits for human health [54]. Therefore, the genetic basis for the accumulation of these useful metabolites from B. distachyon will provide clues for the breeding of cool season crops, such as wheat, barley, rye and oat, in order to improve their nutritional content through comparative analysis between B. distachyon and these Triticeae crops.…”

Section: Discussionmentioning

confidence: 99%

Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon

Onda

Inoue

Sawada

et al. 2019

IJMS

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Metabolite composition and concentrations in seed grains are important traits of cereals. To identify the variation in the seed metabolotypes of a model grass, namely Brachypodium distachyon, we applied a widely targeted metabolome analysis to forty inbred lines of B. distachyon and examined the accumulation patterns of 183 compounds in the seeds. By comparing the metabolotypes with the population structure of these lines, we found signature metabolites that represent different accumulation patterns for each of the three B. distachyon subpopulations. Moreover, we found that thirty-seven metabolites exhibited significant differences in their accumulation between the lines Bd21 and Bd3-1. Using a recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21, we identified the quantitative trait loci (QTLs) linked with this variation in the accumulation of thirteen metabolites. Our metabolite QTL analysis illustrated that different genetic factors may presumably regulate the accumulation of 4-pyridoxate and pyridoxamine in vitamin B6 metabolism. Moreover, we found two QTLs on chromosomes 1 and 4 that affect the accumulation of an anthocyanin, chrysanthemin. These QTLs genetically interacted to regulate the accumulation of this compound. This study demonstrates the potential for metabolite QTL mapping in B. distachyon and provides new insights into the genetic dissection of metabolomic traits in temperate grasses.

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Section: Discussionmentioning

confidence: 99%

Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon

Onda

Inoue

Sawada

et al. 2019

IJMS

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“…A line of common hexaploid wheat with a deep purple grain color (accession no. K4191), developed by Korea University (Republic of Korea), was used in this study [21]. Wheat seeds were grown on a radiation breeding research farm located at 35.5699° N and 126.9722° E (Jeongeup-si, Jeollabuk-do, Korea).…”

Section: Plant Materialsmentioning

confidence: 99%

“…These were BLAST searched against a wheat reference genome sequence (wheat IWGSC Reference Sequence v1.0) provided by URGI (https ://urgi.versa illes .inra.fr/) with an E-value threshold < 1e −5 . We investigated the F-box proteins that regulate anthocyanin biosynthesis during wheat grain development using colored wheat, the seed coat of which starts to change color from green to red on DAF20 [21]. We collected KFBs from a wheat database (Triticum aestivum IWGSC1.0), and putative wheat KFBs were confirmed by the presence of F-box domains in the N-terminus and the kelch repeat domain of the C-terminus using the InterProScan database (https ://www.ebi.ac.uk/inter pro/searc h/seque nce-searc h).…”

Section: Isolation and Sequence Analysis Of Kfbs In Wheatmentioning

confidence: 99%

Isolation and characterization of kelch repeat-containing F-box proteins from colored wheat

et al. 2020

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F-box proteins play important roles in the regulation of various developmental processes in plants. Approximately 1796 F-box genes have been identified in the wheat genome, but details of their functions remain unknown. Moreover, not much was known about the roles of kelch repeat domain-containing F-box genes (TaKFBs) in wheat. In the present study, we isolated five TaKFBs to investigate the roles of KFBs at different stages of colored wheat grain development. The cDNAs encoding TaKFB1, TaKFB2, TaKFB3, TaKFB4, and TaKFB5 contained 363, 449, 353, 382, and 456 bp open reading frames, respectively. All deduced TaKFBs contained an F-box domain (IPR001810) and a kelch repeat type 1 domain (IPR006652), except TaKFB2. Expression of TaKFBs was elevated during the pigmentation stages of grain development. To clarify how TaKFB and SKP interact in wheat, we investigated whether five TaKFB proteins showed specificity for six SKP proteins using a yeast two-hybrid (Y2H) assay. An Y2H screen was performed to search for proteins capable of binding the TaKFBs and interaction was identified between TaKFB1 and aquaporin PIP1. To examine the subcellular localization of TaKFBs, we transiently expressed TaKFB-green fluorescent protein (GFP) fusions in tobacco leaves; the TaKFB-GFP fusions were detected in the nucleus and the cytoplasm. Y2H and bimolecular fluorescence complementation (BiFC) assays revealed that TaKFB1 specifically interacts with aquaporin PIP1. These results will provide useful information for further functional studies on wheat F-box proteins and their possible roles.

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“…The wheat mutant lines were generated by treating the seeds of the original cultivar with 200 Gy of gamma ( 60 Co) irradiation [29]. These mutants were selected according to their diverse phenotypic variants and exhibited a stable inheritance of these phenotypes for 4 years.…”

Section: Plant Materialsmentioning

confidence: 99%

Comparison of Flavonoid Profiles in Sprouts of Radiation Breeding Wheat Lines (Triticum aestivum L.)

et al. 2020

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Wheat (Triticum aestivum Linn.; Poaceae), one of the most popular food crops worldwide, contains basic and essential nutrients and various health benefiting phytochemicals. Among them, flavonoids have attracted significant interest owing to their various health-promoting properties. In this study, 35 wheat mutant lines were developed via gamma-irradiated mutation breeding from the original cultivar. The effects of radiation breeding on the endogenous phytochemical production in the sprouts of these mutant lines were investigated using high performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (HPLC-DAD-ESIMS) with multivariate analysis for the first time in this study. Fourteen characteristic peaks, including eleven flavone C-glycosides, two flavone O-glycosides, and one flavone, were identified. In addition, the contents of three flavone C-glycosides, namely, isoschaftoside, isoorientin, and isoscoparin, in 37 wheat sprout samples from the original cultivar, certificated cultivar, and the mutant lines were determined. A heat map combined with hierarchical clustering showed variation in the relative content for the flavonoids between the 37 wheat sprout samples, clustering into three groups. On principal component analysis scores scatter and loading plots, significant differences in the levels of flavonoids were found between the samples and several markers responsible for group separation were detected. These results provide a scientific reference for the phytochemical variation in wheat mutant lines, thereby aiding in further mutation mechanism studies and for the quality control of the improved wheat cultivars.

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Characterization of novel mutants of hexaploid wheat (Triticum aestivum L.) with various depths of purple grain color and antioxidant capacity

Abstract: Purple grain color is associated with higher anthocyanin accumulation and antioxidant capacity in wheat. Wheat mutants developed in this study may serve as a valuable source of antioxidants. © 2018 Society of Chemical Industry.

Cited by 22 publications

References 48 publications

Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon

Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon

Isolation and characterization of kelch repeat-containing F-box proteins from colored wheat

Comparison of Flavonoid Profiles in Sprouts of Radiation Breeding Wheat Lines (Triticum aestivum L.)

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