Comparison of Flavonoid Profiles in Sprouts of Radiation Breeding Wheat Lines (Triticum aestivum L.)

Abstract

Wheat (Triticum aestivum Linn.; Poaceae), one of the most popular food crops worldwide, contains basic and essential nutrients and various health benefiting phytochemicals. Among them, flavonoids have attracted significant interest owing to their various health-promoting properties. In this study, 35 wheat mutant lines were developed via gamma-irradiated mutation breeding from the original cultivar. The effects of radiation breeding on the endogenous phytochemical production in the sprouts of these mutant lines were investigated using high performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (HPLC-DAD-ESIMS) with multivariate analysis for the first time in this study. Fourteen characteristic peaks, including eleven flavone C-glycosides, two flavone O-glycosides, and one flavone, were identified. In addition, the contents of three flavone C-glycosides, namely, isoschaftoside, isoorientin, and isoscoparin, in 37 wheat sprout samples from the original cultivar, certificated cultivar, and the mutant lines were determined. A heat map combined with hierarchical clustering showed variation in the relative content for the flavonoids between the 37 wheat sprout samples, clustering into three groups. On principal component analysis scores scatter and loading plots, significant differences in the levels of flavonoids were found between the samples and several markers responsible for group separation were detected. These results provide a scientific reference for the phytochemical variation in wheat mutant lines, thereby aiding in further mutation mechanism studies and for the quality control of the improved wheat cultivars.

1H NMR-Based Chemometrics to Gain Insights Into the Bran of Radiation-Induced Colored Wheat Mutant

“…The voucher specimens were deposited at the Radiation Breeding Research Center, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute. For the present study, seeds of the selected mutants (PWr1–37) were sown, germinated, and grown, together with two yellow wheat seeds (YW1 and YW2), in the same manner as previously reported ( 22 ). YW1 and PW were developed from the cross “Woori-mil (Korea RDA accession no.…”

Paper Spray Mass Spectrometry on the Analysis of Phenolic Compounds in Rhynchelytrum repens: A Tropical Grass with Hypoglycemic Activity

“… 1 Identification based on expected mass signals [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. …”

Changes in physio-biochemical metabolism, phenolics and antioxidant capacity during germination of different wheat varieties

“…Flavonoids are important components in plants. It has been reported that flavonoids have health benefits, such as anti-obesity, anti-inflammatory, anti-cardiovascular, anti-cancer and antioxidant activities ( Ah-Reum et al, 2020 ). Studies have shown that wheat sprouts have the effects of enhancing human immunity, antioxidant, anticancer and reducing the risk of chronic diseases, and these health effects were closely related to the unique phenolic substances and their antioxidant properties ( Yu & Beta, 2015 ).…”