Characterization of Ni-tolerant methylobacteria associated with the hyperaccumulating plant Thlaspi goesingense and description of Methylobacterium goesingense sp. nov.

Idris, Rughia; Kuffner, Melanie; Bodrossy, Levente; Puschenreiter, Markus; Monchy, Sébastien; Wenzel, Walter W.; Sessitsch, Angela

doi:10.1016/j.syapm.2006.01.011

Cited by 83 publications

(38 citation statements)

References 35 publications

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“…Previously, we have demonstrated that endophytic bacteria of the zinc hyperaccumulator N. caerulescens show higher zinc tolerance when compared with bacteria pathogenic on related non-accumulator plants [15], an idea supported by other studies of bacteria associated with metal-hyperaccumulating plants [18,19,[38][39][40]. Considered alongside evidence for a role of hyperaccumulated metals in defence of plants such as N. caerulescens against disease [ [13][14][15]41,42], this suggests that local adaptation of pathogens to high-metal concentrations in the rhizosphere and phyllosphere of metal-accumulating plants might drive the evolution of further defensive hyperaccumulation in a form of coevolutionary arms race [15,43].…”

Section: Discussionmentioning

confidence: 53%

Local adaptation is associated with zinc tolerance inPseudomonasendophytes of the metal-hyperaccumulator plantNoccaea caerulescens

et al. 2016

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Metal-hyperaccumulating plants, which are hypothesized to use metals for defence against pests and pathogens, provide a unique context in which to study plant -pathogen coevolution. Previously, we demonstrated that the high concentrations of zinc found in leaves of the hyperaccumulator Noccaea caerulescens provide protection against bacterial pathogens, with a potential trade-off between metal-based and pathogen-induced defences. We speculated that an evolutionary arms race between zinc-based defences in N. caerulescens and zinc tolerance in pathogens might have driven the development of the hyperaccumulation phenotype. Here, we investigate the possibility of local adaptation by bacteria to the zinc-rich environment of N. caerulescens leaves and show that leaves sampled from the contaminated surroundings of a former mine site harboured endophytes with greater zinc tolerance than those within plants of an artificially created hyperaccumulating population. Experimental manipulation of zinc concentrations in plants of this artificial population influenced the zinc tolerance of recovered endophytes. In laboratory experiments, only endophytic bacteria isolated from plants of the natural population were able to grow to high population densities in any N. caerulescens plants. These findings suggest that long-term coexistence with zinc-hyperaccumulating plants leads to local adaptation by endophytic bacteria to the environment within their leaves.

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Section: Discussionmentioning

confidence: 53%

Local adaptation is associated with zinc tolerance inPseudomonasendophytes of the metal-hyperaccumulator plantNoccaea caerulescens

et al. 2016

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“…It could be interesting to look for the presence of Sphingomonas in the endosphere and rhizosphere of other nonaccumulator serpentinophytes to address the presence of specific interactions between hyperaccumulators and Sphingomonas strains. Moreover, members of the genus Methylobacterium were recovered and one species, named Methylobacterium goesingense was found to be associated with the plant (Idris et al 2006). Recently, in an effort to characterize the variability of leaf-associated community between individual plants of A. bertolonii by T-RFLP fingerprinting, we found that, while Te r m i n a l -R e s t r i c t i o n F r a g m e n t s ( T-R F s ) corresponding to Sphingomonas were common to both soils and plants, T-RFs identified as Methylobacteria were present only in foliar DNA extracts ).…”

Section: Bacterial Communities In Serpentine Soilmentioning

confidence: 99%

“…Very promising strains have been isolated from hyperaccumulators and from bulk serpentine soil, which could be good models for serpentine genomics, as for instance Methylobacterium goesingense (Idris et al 2006), some strains from the rhizosphere of Alyssum murale (Abou-Shanab et al 2007a;Abou-Shanab et al 2003b), but also Serratia marcescens C-1 (Marrero et al 2007) or Streptomyces yatensis (Saintpierre et al 2003). Genome sequencing of such strains could provide new important hints for the evolutionary aspects of metal resistance and for unravelling the genetic basis of their positive interactions with hyperaccumulating plants.…”

Section: Perspectivesmentioning

confidence: 99%

Plants as extreme environments? Ni-resistant bacteria and Ni-hyperaccumulators of serpentine flora

2009

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During recent years there has been an increasing interest in the bacterial communities occurring in unusual, often extreme, environments. On serpentine outcrops around the world, a high diversity of plant species showing the peculiar features of metal hyperaccumulation is present. These metal hyperaccumulators have received much attention for their potential biotechnological exploitation in phytoremediation processes, but also as unusual, extreme habitats for the associated bacterial flora, which could reveal novel details concerning bacterial adaptation. This paper will briefly focus on the research topics that have been addressed to date on bacteria associated with serpentine plants and aims to provide a state of the art and to present possible future directions for research which could lead to new insights on microbial adaptation and evolution, and potentially applied in technologies for sustainable use and remediation of contaminated land.

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“…The genus Methylobacterium currently comprises 28 described species (28, 60). Several new species have been described during the last few years, including species from environments that have been analyzed for several decades, such as the plant phyllosphere (23,27,28,38,58). This suggests that the diversity of Methylobacterium is still not fully known.…”

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confidence: 99%

Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis

Knief

Francès

Cantet

et al. 2008

Appl Environ Microbiol

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Bacteria of the genus Methylobacterium are widespread in the environment, but their ecological role in ecosystems, such as the plant phyllosphere, is not very well understood. To gain better insight into the distribution of different Methylobacterium species in diverse ecosystems, a rapid and specific cultivationindependent method for detection of these organisms and analysis of their community structure is needed. Therefore, 16S rRNA gene-targeted primers specific for this genus were designed and evaluated. These primers were used in PCR in combination with a reverse primer that binds to the tRNA Ala gene, which is located upstream of the 23S rRNA gene in the 16S-23S intergenic spacer (IGS). PCR products that were of different lengths were obtained due to the length heterogeneity of the IGS of different Methylobacterium species. This length variation allowed generation of fingerprints of Methylobacterium communities in environmental samples by automated ribosomal intergenic spacer analysis. The Methylobacterium communities on leaves of different plant species in a natural field were compared using this method. The new method allows rapid comparisons of Methylobacterium communities and is thus a useful tool to study Methylobacterium communities in different ecosystems.Bacteria of the genus Methylobacterium are facultative methylotrophs capable of growth on one-carbon compounds, such as methanol, methylamine, formaldehyde, and formate (16). The members of this genus of Alphaproteobacteria are ubiquitous in nature. They have been detected in soil, dust, freshwater, lake sediments, and the air and on plants (16). They have also been found in association with humans (2), and some members of this genus have increasingly been reported to be a cause of opportunistic infections in immunocompromised patients (22). The genus Methylobacterium currently comprises 28 described species (28, 60). Several new species have been described during the last few years, including species from environments that have been analyzed for several decades, such as the plant phyllosphere (23,27,28,38,58). This suggests that the diversity of Methylobacterium is still not fully known. The high diversity of species in the genus Methylobacterium and their broad distribution in nature raises questions of whether different species are adapted to certain environments and whether they play similar ecological roles in different environments. To address these questions, a better understanding of the distribution of Methylobacterium strains between and within different ecosystems and analysis of Methylobacterium community compositions and dynamics in natural habitats are necessary.One of the major habitats of Methylobacterium is represented by plants. Members of this genus have been detected by cultivation-dependent methods (8,12,39,45,59) and, to a lesser extent, by cultivation-independent methods (3,24,25,47,48) as epiphytic and endophytic colonizers of the plant phyllosphere, as intracellular colonizers of the buds of Scots pine (47), and as root-nod...

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Characterization of Ni-tolerant methylobacteria associated with the hyperaccumulating plant Thlaspi goesingense and description of Methylobacterium goesingense sp. nov.

Cited by 83 publications

References 35 publications

Local adaptation is associated with zinc tolerance inPseudomonasendophytes of the metal-hyperaccumulator plantNoccaea caerulescens

Local adaptation is associated with zinc tolerance inPseudomonasendophytes of the metal-hyperaccumulator plantNoccaea caerulescens

Plants as extreme environments? Ni-resistant bacteria and Ni-hyperaccumulators of serpentine flora

Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis

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