Local adaptation is associated with zinc tolerance in<i>Pseudomonas</i>endophytes of the metal-hyperaccumulator plant<i>Noccaea caerulescens</i>

Fones, Helen N.; McCurrach, H.; Mithani, Aziz; Smith, J. Andrew C.; Preston, Gail M.

doi:10.1098/rspb.2016.0648

Cited by 12 publications

(10 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although C . albicans is not a known pathogen of insect larvae, this observation is interesting because it suggests that Galleria may possess a form of high-zinc nutritional immunity; a phenomenon which has been reported in mammals [ 5 ] and, recently, in plants [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

Biphasic zinc compartmentalisation in a human fungal pathogen

et al. 2018

View full text Add to dashboard Cite

Nutritional immunity describes the host-driven manipulation of essential micronutrients, including iron, zinc and manganese. To withstand nutritional immunity and proliferate within their hosts, pathogenic microbes must express efficient micronutrient uptake and homeostatic systems. Here we have elucidated the pathway of cellular zinc assimilation in the major human fungal pathogen Candida albicans. Bioinformatics analysis identified nine putative zinc transporters: four cytoplasmic-import Zip proteins (Zrt1, Zrt2, Zrt3 and orf19.5428) and five cytoplasmic-export ZnT proteins (orf19.1536/Zrc1, orf19.3874, orf19.3769, orf19.3132 and orf19.52). Only Zrt1 and Zrt2 are predicted to localise to the plasma membrane and here we demonstrate that Zrt2 is essential for C. albicans zinc uptake and growth at acidic pH. In contrast, ZRT1 expression was found to be highly pH-dependent and could support growth of the ZRT2-null strain at pH 7 and above. This regulatory paradigm is analogous to the distantly related pathogenic mould, Aspergillus fumigatus, suggesting that pH-adaptation of zinc transport may be conserved in fungi and we propose that environmental pH has shaped the evolution of zinc import systems in fungi. Deletion of C. albicans ZRT2 reduced kidney fungal burden in wild type, but not in mice lacking the zinc-chelating antimicrobial protein calprotectin. Inhibition of zrt2Δ growth by neutrophil extracellular traps was calprotectin-dependent. This suggests that, within the kidney, C. albicans growth is determined by pathogen-Zrt2 and host-calprotectin. As well as serving as an essential micronutrient, zinc can also be highly toxic and we show that C. albicans deals with this potential threat by rapidly compartmentalising zinc within vesicular stores called zincosomes. In order to understand mechanistically how this process occurs, we created deletion mutants of all five ZnT-type transporters in C. albicans. Here we show that, unlike in Saccharomyces cerevisiae, C. albicans Zrc1 mediates zinc tolerance via zincosomal zinc compartmentalisation. This novel transporter was also essential for virulence and liver colonisation in vivo. In summary, we show that zinc homeostasis in a major human fungal pathogen is a multi-stage process initiated by Zrt1/Zrt2-cellular import, followed by Zrc1-dependent intracellular compartmentalisation.

show abstract

Section: Resultsmentioning

confidence: 99%

Biphasic zinc compartmentalisation in a human fungal pathogen

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Inhibitory concentrations were measured using a spectrophotometer at an absorbance of 600 nm against an LB broth (blank) containing the same amount of HM [ 19 ]. The resistance of the novel strains isolated from the ginseng rhizosphere was evaluated by calculating the concentration at which there was 50% inhibition of growth (IC 50 ) [ 20 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

Siderophore-producing rhizobacteria reduce heavy metal-induced oxidative stress in Panax ginseng Meyer

Huo

Kang

Ahn

et al. 2021

Journal of Ginseng Research

View full text Add to dashboard Cite

Background Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119 T had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 10 8 CFU/mL DCY119 T for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119 T can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions It was confirmed that SPR DCY119 T can potentially be used for bioremediation of HM contamination.

show abstract

“…These four populations of N. caerulescens were chosen to represent the range of metal hyperaccumulation phenotypes seen in this species. Prayon is a well-characterized accession, with a hyperaccumulation phenotype that might be considered typical for metalliferous N. caerulescens, and has been used by us in previous studies [13,16,46]. Viviez and Ganges are both metalliferous accessions with high tolerance to zinc, but Ganges is also remarkable in its tolerance and accumulation of cadmium [6,49,50].…”

Section: Methodsmentioning

confidence: 99%

“…To address the first of these hypotheses, we study the growth of Pseudomonas syringae pv. maculicola M4 ( Psm ) and zinc tolerance mutants of this pathogen, and of two naturally occurring endophytes of N. caerulescens [46] in planta in all four populations. To address the second hypothesis, we investigate glucosinolate concentrations and two inducible stress responses previously found to be altered in N. caerulescens , when compared to non-hyperaccumulating species—ROS production and cell death [16].…”

Section: Introductionmentioning

confidence: 99%

Variation in defence strategies in the metal hyperaccumulator plant Noccaea caerulescens is indicative of synergies and trade-offs between forms of defence

Fones

Preston

Smith³

2019

R. Soc. open sci.

Self Cite

View full text Add to dashboard Cite

In the metal hyperaccumulator plant Noccaea caerulescens, zinc may provide a defence against pathogens. However, zinc accumulation is a variable trait in this species. We hypothesize that this variability affects the outcome of interactions between metal accumulation and the various constitutive and inducible defences that N. caerulescens shares with non-accumulator plants. We compare zinc concentrations, glucosinolate concentrations and inducible stress responses, including reactive oxygen species (ROS) and cell death, in four N. caerulescens populations, and relate these to the growth of the plant pathogen Pseudomonas syringae, its zinc tolerance mutants and Pseudomonas pathogens isolated from a natural population of N. caerulescens. The populations display strikingly different combinations of defences. Where defences are successful, pathogens are limited primarily by metals, cell death or organic defences; there is evidence of population-dependent trade-offs or synergies between these. In addition, we find evidence that Pseudomonas pathogens have the capacity to overcome any of these defences, indicating that the arms race continues. These data indicate that defensive enhancement, joint effects and trade-offs between different forms of defence are all plausible explanations for the variation we observe between populations, with factors including metal availability and metal-tolerant pathogen load probably shaping the response of each population to infection.

show abstract

Local adaptation is associated with zinc tolerance inPseudomonasendophytes of the metal-hyperaccumulator plantNoccaea caerulescens

Cited by 12 publications

References 43 publications

Biphasic zinc compartmentalisation in a human fungal pathogen

Biphasic zinc compartmentalisation in a human fungal pathogen

Siderophore-producing rhizobacteria reduce heavy metal-induced oxidative stress in Panax ginseng Meyer

Variation in defence strategies in the metal hyperaccumulator plant Noccaea caerulescens is indicative of synergies and trade-offs between forms of defence

Contact Info

Product

Resources

About