Characterization of New Staphylococcal Cassette Chromosome
 mec
 (SCC
 mec
 ) and Topoisomerase Genes in Fluoroquinolone- and Methicillin-Resistant
 Staphylococcus pseudintermedius

Descloux, Sybill; Rossano, Alexandra; Perreten, Vincent

doi:10.1128/jcm.02255-07

Cited by 137 publications

(154 citation statements)

References 28 publications

Supporting

Mentioning

139

Contrasting

Unclassified

Order By: Relevance

“…This is in line with recent studies showing the acquisition of resistance genes by incorporation of mobile elements in the genome (7). Furthermore, in the very successful CC71 lineage, SCCmec types II and III predominate (15,28), but other types are emerging (7,29).…”

Section: Discussionsupporting

confidence: 72%

Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

et al. 2016

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 72%

Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

et al. 2016

View full text Add to dashboard Cite

“…Investigations of resistance mechanisms and their distribution among staphylococcal populations have been conducted, but further work is needed to fully evaluate their clinical relevance. 3,9 The current study and others also confirm that relative resistance of S. pseudintermedius to oxacillin has increased in recent years. 10,13,15,19,20 Efforts to standardize detection methods, establish shared databases, promote collaborative research, and create venues for sharing knowledge of MRSP will help improve surveillance and reduce the spread of MRSP.…”

Section: Discussionsupporting

confidence: 67%

Evaluation of Susceptibility Test Breakpoints Used to Predict mecA-Mediated Resistance in Staphylococcus Pseudintevmedius Isolated from Dogs

et al. 2009

View full text Add to dashboard Cite

Abstract. Clinical and Laboratory Standards Institute interpretive breakpoints for in vitro susceptibility tests that predict mecA-mediated oxacillin resistance in Staphylococcus pseudintermedius isolates from animals have been changed twice in the past decade. Moreover, there are no counterpart recommendations for human isolates of S. pseudintermedius. Individual medical and veterinary laboratories variably use interpretive breakpoints identical to those recommended for use with Staphylococcus aureus or identical to those recommended for use with coagulase-negative staphylococci. The purpose of the current study was to examine correlations between oxacillin disk diffusion, oxacillin gradient diffusion, oxacillin microbroth dilution, and cefoxitin disk diffusion tests used to predict mecA-mediated resistance in S. pseudintermedius and to retrospectively estimate, from disk diffusion zone diameter measurements, the prevalence and rate of increase of oxacillin resistance among canine S. pseudintermedius isolates submitted to a veterinary teaching hospital laboratory. Oxacillin disk diffusion zone diameters of #17 mm and oxacillin minimum inhibitory concentrations of $0.5 mg/ml were highly correlated with detection of mecA in canine S. pseudintermedius isolates by polymerase chain reaction. MecA-mediated resistance among S. pseudintermedius isolates from dogs increased from less than 5% in 2001 to near 30% in 2007. More than 90% of the methicillin-resistant S. pseudintermedius isolates in 2006 and 2007 were also resistant to representatives of $4 additional antimicrobial drug classes. Cefoxitin disk diffusion with the resistance breakpoint set at #24 mm significantly underestimated the presence of mecA in S. pseudintermedius.

show abstract

“…According to Clinical and Laboratory Standards Institute (CLSI) guidelines, MIC cutoff values for oxacillin for determining methicillin resistance against S. aureus and S. lugdunensis are 4 g/ml and differ from the values for other species (0.5 g/ml) (8). It was previously reported that some strains exhibited oxacillin MICs of 0.5 to Ͻ4 g/ml among mecA-positive S. pseudintermedius strains (4,9,27). If such strains are not identified as S. pseudintermedius strains but are identified as S. aureus strains, they could be misidentified as being methicillin-susceptible strains.…”

Section: Discussionmentioning

confidence: 99%

Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci

et al. 2010

View full text Add to dashboard Cite

In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has been no reliable method to distinguish among CoPS species in veterinary clinical laboratories. In the present study, we sequenced the thermonuclease (nuc) genes of staphylococcal species and devised a multiplex-PCR (M-PCR) method for species identification of CoPS by targeting the nuc gene locus. To evaluate sensitivity and specificity, we used this M-PCR method on 374 staphylococcal strains that had been previously identified to the species level by an hsp60 sequencing approach. We could successfully distinguish between S. aureus, S. hyicus, S. schleiferi, S. intermedius, S. pseudintermedius, and S. delphini groups A and B. The present method was both sensitive (99.8%) and specific (100%). Our M-PCR assay will allow the routine species identification of CoPS isolates from various animal species for clinical veterinary diagnosis.

show abstract

Characterization of New Staphylococcal Cassette Chromosome mec (SCC mec ) and Topoisomerase Genes in Fluoroquinolone- and Methicillin-Resistant Staphylococcus pseudintermedius

Cited by 137 publications

References 28 publications

Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

Evaluation of Susceptibility Test Breakpoints Used to Predict mecA-Mediated Resistance in Staphylococcus Pseudintevmedius Isolated from Dogs

Multiplex-PCR Method for Species Identification of Coagulase-Positive Staphylococci

Contact Info

Product

Resources

About