Characterization of new oligosaccharide converted from cellobiose by novel strain of Bacillus subtilis

Abstract

Six bacterial strains isolated from various Korean fermented foods were cultured in cellobiose-containing medium to investigate their potential for producing new kind of oligosaccharides. After bacterial culture in a liquid medium, each culture medium was concentrated and analyzed. TLC analysis revealed that only one strain ( SS-76) produced new spot on the TLC plate, indicating that it could converts cellobiose into a new oligosaccharide. Following purification of the culture supernatant of SS-76, the fractions containing the oligosaccharide produced were pooled, and the concentrated fraction was analyzed for its chemical and structural characteristics. By using various analytical techniques such as sugar composition analysis, glycosidic linkage analysis, and molecular weight determination, the new oligosaccharide was identified as glucotriose connected with (1 → 4) and (1 → 3) glycosidic linkages. In addition, the result of specific enzyme catalysis suggested that the new glucotriose might contain only β-configurations in their anomeric configurations.

The effects of environmental complexity on microbial community yield and structure

“…They do not, however, explain how individual carbon sources behave in higher-order combinations. For example, though the community grown on D-cellobiose displayed high taxonomic diversity (possibly sustained via cross-feeding of L-lactate from B. subtilis 39,40 ), all organisms were overtaken by P. aeruginosa when D-cellobiose was combined with glycerol ( Figure 3a). We therefore generated a simple linear model to determine whether any particular carbon sources were more universally associated with higher taxonomic diversity.…”

“…Despite our observation that an organism's performance in monoculture is rarely correlated with its persistence in a community ( Supplementary Figure 19), the ability of B. subtilis to efficiently metabolize D-cellobiose ( Supplementary Figure 2a) may provide clues to its high abundance in this case. Specifically, B. subtilis has been documented to convert cellobiose to L-lactate 47,48 . Our initial phenotypic assays showed that P. aeruginosa and S. oneidensis, the next most prevalent organisms in this community, can utilize L-lactate, indicating the possibility of cross-feeding.…”

Production Optimization, Structural Analysis, and Prebiotic- and Anti-Inflammatory Effects of Gluco-Oligosaccharides Produced by Leuconostoc lactis SBC001

“…lactis CCK940 exhibited prebiotic effects on Lactobacillus casei , L. pentosus , L. plantarum , W. cibaria , B. animalis , and S. cerevisiae , which was different from this study result [ 17 ]. Several other studies have shown that each prebiotic oligosaccharide has its own prebiotic effect on specific probiotic strains [ 36 , 37 , 38 ]. The results of these studies indicate that the prebiotic effects of lactic acid strains vary depending on the type and structure of the oligosaccharides produced.…”

Hyper β-glucosidase producer Beauveria bassiana SAN01—optimization of fermentation conditions and evaluation of saccharification potential

“…bassiana SAN01 Bgl was partially purified and concentrated via (NH 4 ) 2 SO 4 precipitation [31] and utilized in the hydrolysis of cellobiose. The 20 mL reaction mixture contained cellobiose at a final concentration of 1700 µg/mL and 50 U/mL of the B. bassiana Bgl in 0.1 mM sodium acetate buffer (pH 5.0) [32]. The reaction was performed for 24 h at 35 °C, samples were withdrawn at intervals, and the reaction was terminated by heating for 5 min at 100 °C in a boiling water bath.…”