Characterization of NDM-5-positive extensively resistant Escherichia coli isolates from dairy cows

He, Tao; Wei, Ruicheng; Zhang, Lili; Sun, Lichang; Pang, Maoda; Wang, Ran; Wang, Yan

doi:10.1016/j.vetmic.2017.06.010

Cited by 53 publications

(41 citation statements)

References 23 publications

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“…enterica Indiana 4 8 NA Broth microdilution bla NDM-1 Fischer et al [83] 1 Salmonella enterica subsp. enterica 0.25 0.125 0.5 E-test bla NDM-1 Shaheen et al [74] 6 Escherichia coli NA 0.5e16 NA Broth microdilution bla NDM-1 Pruthvishree et al [46] 1 Escherichia coli NA 128 NA E-test bla NDM-5 He et al [30] 10 Klebsiella pneumoniae 128e256 128e256 NA Broth microdilution bla NDM-5 He et al [31] 3 Escherichia coli NA 128 NA Broth microdilution bla NDM-5 Yaici et al [18] 4 Escherichia coli >32 >32 >32 E-test bla NDM-5 Ghatak et al [44] 1 Escherichia coli >8 >8 NA Phoenix system bla NDM-5 Wang et al [25] 64 Escherichia coli 2e512 4->512 NA Agar dilution bla NDM-5 Yousfi et al [67] 1 Escherichia coli >32 >32 >32 E-test bla NDM-5 Wang et al [25] 47 Escherichia coli 1e256 2e512 NA Agar dilution bla NDM-9 Yao et al [24] 1 Escherichia coli 8 16 NA Unspecified bla NDM-9 Liu et al [27] 2 Cronobacter sakazakii 128 32e64 NA Broth microdilution bla NDM-9 Liu et al [28] 1 Antimicrobial susceptibility test (AST) results are shown for IMI, imipenem; MER, meropenem; ERT, ertapenem; NA, not available. MICs are shown in mg/L and were identical for all isolates tested, if not specified (otherwise, the MIC range is shown); * after several weeks of cultivation; initially test results were 0.25 for both IMI and MER.…”

Section: Discussionmentioning

confidence: 99%

“…The following carbapenemase genes were [19,35,36,48e50], and imipenem-carbapenemase (IMP-1, IMP-2, IMP-27) ( (Table 1). Overall, carbapenemase-producing CRE were isolated from poultry (meat) [25,36], chicken, poultry, or fowl (meat) [19,22,24,26e28,34,40,50], pig (meat) [29,39e42,46,48,60], cows or raw milk [18,30,31,44,49], cattle [35], and various types of seafood [62,64,65].…”

Section: Occurrence Of Cre In Livestock and (Sea)foodmentioning

confidence: 99%

See 1 more Smart Citation

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Köck

Daniels-Haardt²,

Becker

et al. 2018

Clinical Microbiology and Infection

253

167

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Section: Discussionmentioning

confidence: 99%

Section: Occurrence Of Cre In Livestock and (Sea)foodmentioning

confidence: 99%

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Köck

Daniels-Haardt²,

Becker

et al. 2018

Clinical Microbiology and Infection

253

167

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“…Plasmids pNDM-K725, pNDM-CR33, and pNDM-Z214 were mostly identical to each other but were slightly different from pNDM-Z244. We speculated that E. coli Z244 possibly acquired the bla NDM-5 gene from commonly reported plasmids like pNDM-MGR194, which can also be found in strains isolated from environmental, animal, and human clinical samples (12,14,28). A study revealing that NDM-5-producing E. coli ST167 was simultaneously detected in a companion dog and his owners in a family in Finland (29) indicated that human-to-canine transmission is possible.…”

Section: Discussionmentioning

confidence: 99%

“…The NDM-5 enzyme differs from NDM-1 by only two amino acid substitutions (Val88Leu and Met154Leu) and shows increased resistance to carbapenems and broad-spectrum cephalosporins (4). It is a concern that bla NDM-5 was detected in not only clinical specimens but also animals (12,13) and environmental samples (14), indicating its potential to spread further in the community. The bla NDM-5 gene was reported to be carried in different incompatibility typing plasmids to transfer genes such as IncFII, IncX3, IncN, and IncF (15).…”

mentioning

confidence: 99%

Dissemination of the bla _NDM-5 Gene via IncX3-Type Plasmid among Enterobacteriaceae in Children

Tian

Wang

Zhang

et al. 2020

mSphere

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The continuous emergence of novel New Delhi metallo-␤-lactamase-5 (NDM-5)-producing Enterobacteriaceae isolates is receiving more and more public attention. Twenty-two NDM-5-producing strains were identified from 146 carbapenemase-producing Enterobacteriaceae (CRE) strains isolated from pediatric patients between January and March 2017, indicating that the bla NDM-5 gene has spread to children. All 22 isolates, including 16 Klebsiella pneumoniae strains, four Klebsiella aerogenes strains, and two Escherichia coli strains, showed significantly high resistance to ␤-lactam antibiotics (except aztreonam) but remained susceptible to tigecycline and colistin. K. pneumoniae and K. aerogenes strains were respectively defined as homologous clonal isolates by pulsed-field gel electrophoresis (PFGE). Multilocus sequence typing (MLST) results confirmed the genetic relatedness with all K. pneumoniae strains belonging to sequence type (ST) 48. Two E. coli isolates (ST617 and ST1236) were considered genetically unrelated. Twenty-two bla NDM-5 plasmids were positive for the IncX3 amplicon and showed almost identical profiles after digestion with HindIII and EcoRI. Four representative strains (K. pneumoniae K725, K. aerogenes CR33, E. coli Z214, and E. coli Z244) were selected for further study. Plasmids harboring bla NDM-5 showed strong stability in both clinical isolates and transconjugants, without apparent plasmid loss after 100 serial generations. S1-PFGE followed by Southern blot analysis demonstrated that the bla NDM-5 gene was located on an ϳ46-kb plasmid. Plasmid sequences of pNDM-K725, pNDM-CR33, and pNDM-Z214 were almost identical but were slightly different from that of pNDM-Z244. Compared with pNDM-Z244, ΔISAba125 and partial copies of IS3000 were missing. The genetic backgrounds of the bla NDM-5 gene in four strains were slightly different from that of the typical pNDM_MGR194. This study comprehensively characterized the horizontal gene transfer of the bla NDM-5 gene among different Enterobacteriaceae isolates in pediatric patients, and the IncX3-type plasmid was responsible for the spread. IMPORTANCE The emergence of CRE strains resistant to multiple antibiotics is considered a substantial threat to human health. Therefore, all the efforts to provide a detailed molecular transmission mechanism of specific drug resistance can contribute positively to prevent the further spread of multidrug-resistant bacteria. Although the new superbug harboring bla NDM-5 has been reported in many countries, it was mostly identified among E. coli strains, and the gene transfer mechanism has not been fully recognized and studied. In this work, we identified 22 bla NDM-5 -positive strains in different species of Enterobacteriaceae, including 16 Klebsiella pneumoniae strains, four Klebsiella aerogenes strains, and two Escherichia coli strains, which indicated the horizontal gene transfer of bla NDM-5 among Enterobacteriaceae strains in pediatric patients. Moreover, bla NDM-5 was located on a 46-kb IncX3 plasmid, which is possibly respon...

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“…The emergence of carbapenem-and colistin-resistance determinants in single strains ushers in a new age of pandrug resistance, and underscores the rapid depletion of our antimicrobial armamentarium (Delgado-Blas, Ovejero, Abadia-Patino, & Gonzalez-Zorn, 2016;He et al, 2017;Sun et al, 2016;Yang et al, 2016). The detection of plasmids harboring both carbapenem and colistin resistance genes has made colistin resistance, which was hitherto solely mediated by mutations in and insertional inactivation of phoPQ, ccrB, mgrB, pmrAB, lpxACD, and pmrHFIJKLM, and restricted to chromosomal or vertical transmission, a grave threat to clinical medicine (Beceiro et al, 2014;Cannatelli et al, 2014;Giani et al, 2015;Jayol, Nordmann, Brink, et al, 2017;Jayol, Nordmann, Lehours, Poirel, & Dubois, 2017;Mavroidi et al, 2015;Wright et al, 2015).…”

mentioning

confidence: 99%

Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods

Sekyere

2018

MicrobiologyOpen

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Resistance to colistin, mediated by chromosomal mutations and more recently, by plasmid-borne mcr genes, is increasingly being reported in bacterial isolates taken from humans, animals, farms, foods, and the environment. To easily identify and contain this quickly spreading menace, efficient diagnostics that are cheaper, faster, simpler, sensitive, and specific have become indispensable and urgently necessary. A thorough and systematic review of the literature available at Pubmed, ScienceDirect and Web of Science was thus undertaken to identify articles describing novel and efficient colistin resistance- and mcr gene-detecting methods. From the final 23 studies included in this review, both phenotypic and molecular tests were found. The phenotypic tests consisted of novel culture media viz., SuperPolymyxin™, CHROMagar COL-APSE and LBJMR media, commercial automated MIC-determining instruments such as MICRONAUT-S, Vitek 2, BD Phoenix, Sensititre and MicroScan, and novel assays such as Colistin MAC test, Colispot, rapid polymxin NP test (RPNP), alteration of Zeta potential, modified RPNP test, MICRONAUT-MIC Strip, MIC Test Strip, UMIC System, and Sensitest™ Colistin. Molecular diagnostics consisted of the CT103XL microarray, eazyplex SuperBug kit, and Taqman /SYBR Green real-time PCR assays, with 100% sensitivity and specificity plus a shorter turnaround time (<3 hr). Based on the sensitivity, specificity, cost, required skill and turnaround time, the RPNP test and/or novel culture media is recommended for under-resourced laboratories while the Multiplex PCR or Taqman /SYBR Green real-time PCR assay alongside the RPNP or novel culture media is suggested for well-resourced ones.

show abstract

Characterization of NDM-5-positive extensively resistant Escherichia coli isolates from dairy cows

Cited by 53 publications

References 23 publications

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Dissemination of the bla _NDM-5 Gene via IncX3-Type Plasmid among Enterobacteriaceae in Children

Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods

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Characterization of NDM-5-positive extensively resistant Escherichia coli isolates from dairy cows

Cited by 53 publications

References 23 publications

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Dissemination of the bla NDM-5 Gene via IncX3-Type Plasmid among Enterobacteriaceae in Children

Mcr colistin resistance gene: a systematic review of current diagnostics and detection methods

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Dissemination of the bla _NDM-5 Gene via IncX3-Type Plasmid among Enterobacteriaceae in Children