Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque

Escobar, Cynthia; Horna, Yuri; Carreño, Carmen; Mendoza, Gilmar

doi:10.17268/sci.agropecu.2011.01.05

Cited by 7 publications

(3 citation statements)

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“…The indirect ammonium ion method was used to evaluate the ability of the two bacterial strains to fix nitrogen, using the Berthelot colorimetric technique (phenol-hypochlorite), as described by Escobar et al (2011). A spectrophotometer was used for the absorbance reading (632.9 nm), considering all strains with positive readings as fixative.…”

Section: Nitrogen Fixation Evaluationmentioning

confidence: 99%

Rhizobacteria inoculation and its effect on the productive parameters of sorghum

Hidalgo-de León,

Sáenz-Mata,

Véliz-Deras

et al. 2024

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Objective: To evaluate the effect of the Bacillus megaterium and Pseudomonas japonica rhizobacteria on the productive parameters of sorghum. Design/Methodology/Approach: The experiment was carried out in Padilla, Tamaulipas, where the effect of inoculating a sorghum crop with the Bacillus megaterium KN13 and Pseudomonas japonica KC14 strains on its productive parameters was evaluated. Both strains were used in two concentrations (106 and 107 CFU). A randomized block design was applied, consisting of five treatments (two strains × two concentrations, plus a control), with six and nine replications. Results: The B. megaterium and P. japonica strains can fix nitrogen and produce siderophores. Inoculating these strains into the sorghum crop increases grain yield, plant height, panicle length, plant stem diameter, and aerial dry weight. Better results are recorded when the strains have a 107 CFU concentration. Study Limitations/Implications: Each type of soil and crop has various microbiomes. Findings/Conclusions: The use of an adequate concentration of rhizobacteria improves sorghum production; therefore, it is a sustainable alternative, both for the nutrition of the crop and the reduction of the use of synthetic fertilizers.

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Section: Nitrogen Fixation Evaluationmentioning

confidence: 99%

Rhizobacteria inoculation and its effect on the productive parameters of sorghum

Hidalgo-de León,

Sáenz-Mata,

Véliz-Deras

et al. 2024

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“…the Molybdite technique (Escobar et al, 2011). Bacterial cultures (5 %; 0.25 mL) of each isolate were prepared in 5mL of National Botanical Research Institute's Phosphate Broth (NBRIP) with 0.85 M of NaCl and incubated at 30 °C daily agitated for 5 min.…”

Section: Phosphate Solubilization Phosphate Solubilization Capacity Was Determined Bymentioning

confidence: 99%

Molecular identification of rhizobacterial isolates from Prosopis limensis and their effect on the growth of Raphanus sativus under salt stress

Clavo

Suyón

Farroñan

et al. 2021

Preprint

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Soil salinity negatively affects the development of agricultural crops. The utilization of plant growth-promoting rhizobacteria is a biotechnological alternative to mitigate this problem. Rhizobacteria were isolated from the roots and rhizosphere of Prosopis limensis Bentham carob to identify them and determine their potential as plant growth-promoters under salt stress. First, ACC deaminase activity was determined in Dworkin & Foster (DF) minimum medium with 3.0 mM ACC as a source of nitrogen; besides, tolerance to sodium chloride was determined in Nutrient Broth (NB) supplemented with 10% NaCI. Then, bacteria displaying ACC deaminase activity and a 10% NaCI tolerance were selected, identified through genomic analysis targeting the 16s rRNA gene, and inoculated into Raphanus sativus L. radish seeds to determine the effect on germination percentage under salt stress (80 mM NaCI) and, also on indole production and phosphate solubilization. Isolates were also utilized to evaluate their effect on the growth of radish in saline soils. Genomic analysis showed two bacterial isolates from the genus Pseudomonas and one from the genus Bordetella: Isolate MW604823 was identified as Pseudomonas sp.; isolate MW604824, as Pseudomonas hunanensis; and isolate MW604826 as Bordetella muralis. Thee isolates demonstrated ACC deaminase activity and tolerance to 10% NaCI. Inoculation of the isolates on radish seeds increased germination percentage compared to the control. The isolates displayed in vitro indole production and phosphate solubilization capacity. Moreover, the isolates promoted the growth of radish under salt stress conditions, increasing, leave number, root number, aerial, and root biomass, demonstrating their potential as a biofertilizers.

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“…One alternative for biofertilization is based on plant-growth-promoting rhizobacteria (PGPR). Some rhizobacteria, as part of their metabolism, increase fertility and benefit plants; crop growth can be affected by mechanisms such as phosphate solubilization, phytohormone production, antibiotics, and other vegetative biostimulant compounds [5]. There are already some commercial rhizobacteria that the tomato seedling producer has turned to.…”

Section: Introductionmentioning

confidence: 99%

Morphophysiological, Enzymatic, and Elemental Activity in Greenhouse Tomato Saladette Seedlings from the Effect of Plant Growth-Promoting Rhizobacteria

et al. 2021

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The tomato is a widely cultivated and consumed vegetable globally. Comarca Lagunera is an important tomato-exporting region of Mexico. Salinity is an abiotic factor that reduces productivity and increases production costs. To advance growing period, there is high demand for the sustainable production of seedlings. Plant-growth-promoting rhizobacteria (PGPR) are characterized by improving plant growth through different mechanisms and can be an option for reducing the misuse of chemical fertilizers. The importance of the application of strains, evaluating various inoculation methods (in seed, soil, foliar spraying, and root immersion), should be evaluated to propose biofertilization packages in a specific crop. Thus, the study aimed to determine the effect of PGPR (Bacillus paralicheniformis, Acinetobacter guillouiae, Aeromonas caviae, and Pseudomonas lini) vs. nutrient solution and distilled water in the seedlings stage of saladette-type tomato on morphophysiological variables, nitrate reductase (NR) enzyme activity, and plant minerals via tissue analysis under greenhouse conditions. The four PGPR were inoculated by different methods (inoculation in seed, sprinkling, and both) in saladette-type tomato seedlings under greenhouse conditions and evaluated in vivo 40 days after sowing for morphophysiological variables, such as seedling height; stem diameter; root displaced volume; fresh and dry weight matter of the leaves, stems, and roots; leaf area; and nitrate reductase enzyme activity. The effect of the inoculation of PGPR showed significant results for Pseudomonas lini vs. the control, with 40% higher values, on average, for plant height, stem diameter, displaced root volume, and fresh weight of root, leaf, and stem. The response of enzymatic and mineral content in seedlings was variable with nutrient solution and significant with distilled water. Studies related to the promotion of plants in the subsequent phenological stages of a tomato, considering the selected PGPR, should be considered in future research.

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Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque

Cited by 7 publications

References 0 publications

Rhizobacteria inoculation and its effect on the productive parameters of sorghum

Rhizobacteria inoculation and its effect on the productive parameters of sorghum

Molecular identification of rhizobacterial isolates from Prosopis limensis and their effect on the growth of Raphanus sativus under salt stress

Morphophysiological, Enzymatic, and Elemental Activity in Greenhouse Tomato Saladette Seedlings from the Effect of Plant Growth-Promoting Rhizobacteria

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