Characterization of myosin light chain in shrimp hemocytic phagocytosis☆

Crustacean hematopoiesis

“…Although these experiments are primarily performed in vivo and do not provide information about the specific functions of different hemocyte types, these studies have clarified the roles of some proteins in hemocyte functions, such as phagocytosis (Han et al, 2010;Wang et al, 2014), coagulation (Fagutao et al, 2012;Maningas et al, 2008) and viral defense Visetnan et al, 2015)). These techniques have also been successfully used to confirm the important role of the proPO-activating system as an early defense system Charoensapsri et al, 2009;Fagutao et al, 2009;Jang et al, 2011).…”

Blocking the large extracellular loop (LEL) domain of FcTetraspanin-3 could inhibit the infection of white spot syndrome virus (WSSV) in Chinese shrimp, Fenneropenaeus chinensis

“…Some recent evidence suggests that targeting WSSV envelop protein vp28 by RNAi technology should be therapeutically beneficial [57]. And some host immune related genes were also discovered to involve in WSSV infection by RNAi, such as caspase [58], myosin light chain [59], antilipopolysaccharide factor [60] and GTPase protein [61]. Moreover, the function of tetraspanins in human or mammal immunity by RNAi had been studied a lot [62].…”

Molecular characterization of a Ran isoform gene up-regulated in shrimp immunity

“…For RT-PCR, the method described previously was followed (Han et al, 2010a(Han et al, , 2010b. Briefly, the PCR reaction mixture (20 μl) consisted of 10 μl of 2 × SYBR Green I real-time PCR Master Mix (TOYOBO, Japan), the diluted cDNA and 0.5 μl of each primer.…”

“…Shrimp, like other invertebrates, lacks a true adaptive immune response system and its disease resistance relies on innate defense system including a series of humoral and cellular immune factors (Soderhall, 1999). Nowadays it becomes clear that the innate immune system plays a critical role in host defense reaction (Han et al, 2010a(Han et al, , 2010bLuo et al, 2003).…”

“…Ran GTPase, belonging to the small G protein superfamily (Ras, Rho, Rab, Arf and Ran families), is highly conserved in eukaryotes from yeast to humans (Han et al, 2010a(Han et al, , 2010b. Ran GTPase is well known for controlling both the rate and directionality of nucleo-cytoplasmic transport (Hetzer et al, 2002).…”