Characterization of Mycobacterium tuberculosis of Lebanese patients by double-repetitive-element polymerase chain reaction

Hamzé, Monzer; Rahmo, Abdulkader; Saade, Marie-Béatrice

doi:10.26719/2010.16.8.812

Cited by 8 publications

(1 citation statement)

References 25 publications

(3 reference statements)

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“…In a previous study, characterization of M. tuberculosis in Lebanese patients by double-repetitive-element polymerase chain reaction (DRE-PCR) indicated the presence of several genotypes with evidence of certain groups specific to geographic areas, implicating separate evolution of M. tuberculosis strains [9]. This investigation reports on the first nationwide study to characterize by spoligotyping clinical isolates of M. tuberculosis from different regions of Lebanon.…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium tuberculosis spoligotypes circulating in the Lebanese population: a retrospective study

Bedrossian

Hamzé²,

Ak³

et al. 2013

East Mediterr Health J

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Genotyping Mycobacterium tuberculosis in Lebanon on the national level may be beneficial for assessing patients and monitoring the therapeutic response to DOTS. This study aimed to characterize the spoligotypes of clinical isolates of M. tuberculosis patients collected between April 2004 and October 2005 from all Lebanese provinces. Isolates (n = 60) were cultured and identified by their biochemical characteristics. DNA extracts of these samples were amplified by PCR and genotyped by spoligotyping. Thirteen (13) patterns of M. tuberculosis complex family strains were identified: 41.6% of the strains belonged to the T 1 family, 25.0% to LAM 9, 10.0% to Haarlem 3, 3.3% to each of CAS, LAM 8, BCG and Family 36 and 1.7% to each of Haarlem 1, LAM 10, S, M. africanum, X 1 and T 3 families. The noticeable absence of Beijing and East African Indian families was not consistent with the patterns reported in neighbouring countries. A more inclusive study of the Lebanese population is necessary to accurately identify most of the prevailing families in the country. Les isolats (n = 60) ont été mis en culture puis identifiés en fonction de leurs caractéristiques biochimiques. Les extraits d'ADN de ces échantillons ont été amplifiés par PCR puis génotypés par spoligotypage. Treize (13) souches différentes de la famille du complexe M. tuberculosis ont été identifiées : 41,6 % des souches appartenaient à la famille T 1 ; 25,0 % à la famille LAM 9 ; 10,0 % à la famille Haarlem 3 ; 3,3 % chacune aux familles CAS, LAM 8, BCG et à la famille 36 et 1,7 % chacune aux familles Haarlem 1, LAM 10, S, M. africanum, X 1 et T 3. L'absence remarquable des familles Beijing, indiennes et d'Afrique de l'Est ne coïncidait pas avec les tendances rapportées dans les pays voisins. Une étude plus globale de la population libanaise est nécessaire pour identifier avec précision la plupart des familles dominantes dans notre pays.

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Section: Introductionmentioning

confidence: 99%

Mycobacterium tuberculosis spoligotypes circulating in the Lebanese population: a retrospective study

Bedrossian

Hamzé²,

Ak³

et al. 2013

East Mediterr Health J

Self Cite

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Testing for the presence of Mycobacterium tuberculosis Beijing genotype strains in Syrian samples

Rahmo¹,

Alsalamah²,

Habous³

2012

International Journal of Mycobacteriology

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The Beijing family of Mycobacterium tuberculosis (MTB) has been reported to have an exceptional capacity to spread tuberculosis (TB) and induce multi-drug resistance. A method has been developed to distinguish this family from the rest of the MTB families through real-time DNA amplification and subsequent analysis of the melting point of an amplicon. Two pools of multi-drug resistant (MDR) MTB samples collected at two different time periods from various regions in Syria have been selected. This preliminary screening indicated a complete absence of the Beijing family in all samples. This research presents an effective differentiation of bacterial Beijing strains, with minimal effort and cost through analysis of differential amplicon melting points.

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Mycobacterium tuberculosis spoligotypes circulating in the Syrian population: A retrospective study

Bedrossian¹,

Rahmo²,

Karam³

et al. 2013

International Journal of Mycobacteriology

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Characterization of Mycobacterium tuberculosis of Lebanese patients by double-repetitive-element polymerase chain reaction

Cited by 8 publications

References 25 publications

Mycobacterium tuberculosis spoligotypes circulating in the Lebanese population: a retrospective study

Mycobacterium tuberculosis spoligotypes circulating in the Lebanese population: a retrospective study

Testing for the presence of Mycobacterium tuberculosis Beijing genotype strains in Syrian samples

Mycobacterium tuberculosis spoligotypes circulating in the Syrian population: A retrospective study

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