Characterization of Mycobacterium tuberculosis isolates from Hebei, China: genotypes and drug susceptibility phenotypes

Li, Yanan; Cao, Xinrui; Li, Shiming; Wang, Hao; Wei, Jianlin; Liu, Peng; Wang, Jing; Zhang, Zhi; Gao, Han; Li, Machao; Wan, Kanglin; Dai, Erfu

doi:10.1186/s12879-016-1441-2

Cited by 27 publications

(28 citation statements)

References 28 publications

(28 reference statements)

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“…Those studies considered that Beijing genotype was associated with drug resistance and those strains showed higher drug resistance level 20 , 21 . Meanwhile, other studies also showed no clearly association relationship between drug resistance and strains clade 13 , 22 .…”

Section: Discussionmentioning

confidence: 93%

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The study on the association between Beijing genotype family and drug susceptibility phenotypes of Mycobacterium tuberculosis in Beijing

Liu

Jiang

et al. 2017

Sci Rep

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The predominant prevalent Mycobacterium tuberculosis (M. tb) lineage was the Beijing genotype family in Beijing. There has been no systematic study on the association between drug resistance and Beijing genotype. Here we collected 268 M. tb strains, analyzed the background information and the bacteriological characteristics. The mean age of the cases was 40.12 years; male patients were almost three times than female patients. After genotyping analyzation, 81.7% (219/268) strains were categorized as Beijing genotype; no significant difference was observed between Beijing and non-Beijing genotype in gender, age and treatment history. Drug susceptibility testing (DST) analyzation demonstrated that 172 (64.2%) strains were fully sensitive to all drugs (Isoniazid, Rifampin, Streptomycin, and Ethambutol), while 96 (35.8%) strains were resistant to at least one of the drugs. Beijing genotype strains exhibited a significantly higher clustering rate. However, no significant association relationship was observed between drug resistance and Beijing genotype family. The study provided insights into the genotype diversity and revealed that the frequencies of drug-resistance of Beijing genotype strains.It would be helpful for the establishment of the efficient tuberculosis (TB) prevention and control strategy in Beijing.

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Section: Discussionmentioning

confidence: 93%

“…Different results obtained from different studies and different regions possibly because of the amount of Beijing genotype sublineages and the percentage of modern or ancient Beijing genotype, which affected the DST results 22 . In 2006 Mokrousov et al .…”

Section: Discussionmentioning

confidence: 99%

The study on the association between Beijing genotype family and drug susceptibility phenotypes of Mycobacterium tuberculosis in Beijing

Liu

Jiang

et al. 2017

Sci Rep

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“…And they were identified as the causative agents of tuberculosis in the world. For each families of MTB resistance to rifampicin, the prevalence was analyzed for the selected studies as follows: Beijing nineteen studies [14, 15, 16, 17, 18, 19, 20, 21, 22, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31], LAM ten studies [15, 17, 23, 25, 28, 29, 32, 33, 34, 35], T ten studies [3, 15, 16, 17, 23, 27, 28, 32, 34, 35], EAI eight studies [18, 22, 25, 27, 28, 29, 33, 36], Ural four studies [15, 17, 30, 34] Haarlem five studies [15, 23, 25, 28, 34], CAS four studies [15, 29, 30, 35] and MANU two studies [16, 29]. The proportion of rifampicin resistance varies by the type of regions and strains.…”

Section: Resultsmentioning

confidence: 99%

“…Eigteen studies reported rifampicin resistance Beijing family. Of these studies, nine were conducted in Western Pacific Region, reported 1309 (97.68%) resistance Beijing family [2, 16, 20, 21, 22, 28, 29, 30, 31], three studies were conducted in South-East Asia Region, reported 15 (1.11%) resistance Beijing family [15, 19, 25], two studies were conducted in European Region and identified 14 (1.04%) resistance Beijing family [17, 24] and two studies were conducted in Region of American but only two resistance Beijing family [18, 23]. One study which was conducted in African Region [29] and one study conducted in Eastern-Mediterranean Region did not report a rifampicin resistance Beijing family [30].…”

Section: Resultsmentioning

confidence: 99%

Resistance of Mycobacterium tuberculosis strains to Rifampicin: A systematic review and meta-analysis

Feyisa

Abdurahman

Jimma

et al. 2019

Heliyon

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IntroductionAntitubercular drug resistance strain is a horrifying barrier to effective TB treatment and prevention. The present study aimed to determine the prevalence and geographical distribution of rifampicin-resistance M. tuberculosis (MTB) strains.MethodsWe searched two electronic databases, PubMed and EMBASE, until 26 March 2017 and updated our search on 27 April 2018 and accessed all prevalence studies of MTB strain and their drug susceptibility patterns to rifampicin. The pooled prevalence estimate was determined using random effects model.ResultsWe identified 23 studies satisfying the inclusion criteria. The proportion of rifampicin resistance strains was diverged depending on the type of strains, country and Regions. The pooled estimate of rifampicin-resistance strains of MTB for the included studies was 4% (95% CI: 3–5%). In subgroup analysis based on World Health Organization (WHO) Regions, the pooled estimate of rifampicin-resistance strains of MTB was 11% (95% CI: 9–13%) with the Western Pacific Region 24%, Europian Region 10%, South-East Asian Region 6%, African Region 3% and Region of American 1%. Beijing family was the most dominant strain resistance to rifampicin with pooled prevalence of 14% (95% CI: 10–18%). The pooled prevalence of other families, i.e. EAI, T, CAS, MANU, Haarlem, LAM and Ural, was ≤2% for each.ConclusionHigh burden of rifampicin resistance MTB strains was identified in the Western Pacific Region. Of these, Beijing family was predominantly resistance to rifampicin in Western Pacific Region and South-East Asian Region and also spread to European Region and Region of American.

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“…Genomic DNA was extracted from mycobacterial colonies grown on L-J medium using a Mericon DNA Bacteria kit (Qiagen, USA), as previously described (30). The expected embB fragment (from codons 207 to 445) was amplified using the following primers: forward-embB (5=-ATC GACACCCGGTTCTCC-3=) and reverse-embB (5=-CTGCACACCCAGT GTGAATG-3=).…”

Section: Methodsmentioning

confidence: 99%

Association between embB Codon 306 Mutations, Phenotypic Resistance Profiles, and Genotypic Characterization in Clinical Mycobacterium tuberculosis Isolates from Hebei, China

Wang

Zhi

et al. 2016

Antimicrob Agents Chemother

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b Ethambutol (EMB) is an essential first-line drug for tuberculosis (TB) treatment. Nucleotide substitutions at embB codon 306 (embB306) have been proposed to be a potential marker for EMB resistance and a predictor of broad drug resistance in clinical Mycobacterium tuberculosis isolates. However, discordant findings about the association between embB306 mutations and EMB resistance were reported. Hebei Province is located in the Beijing-Tianjin-Hebei integration region in China; however, little information about the genetic diversity of the embB locus in this area is available. In this study, we sequenced the region surrounding embB306 (codons 207 to 445) in 62 ethambutol-resistant (EMB r ) isolates, 214 ethambutol-susceptible isolates resistant to other first-line drugs (EMB s isolates), and 100 pan-sensitive isolates. Our data indicated that none of the pan-sensitive isolates showed mutations at embB306 and 63 drug-resistant isolates harbored embB306 substitutions, with these substitutions being found in 56.5% (35/62) of EMB r isolates and 13.1% (28/214) of EMB s isolates. A significant association between the embB306 mutation and resistance to isoniazid, rifampin, EMB, and multiple drugs was observed, and the rate of mutation of embB306 increased with increasing numbers of first-line drugs to which the isolates were resistant. The embB306 mutation is not the sole causative factor for EMB resistance, and the poor sensitivity limits its utility as a marker for drug-resistant TB. However, it may be a potential marker for broad drug resistance, especially for multidrug resistance. The mycobacterial interspersed repetitive unit-variable-number tandem-repeat profiles may serve as markers for predicting the embB306 substitutions that may occur in drug-resistant M. tuberculosis isolates under antimicrobial selection pressure.T uberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. According to estimates of the World Health Organization (WHO), in 2014 there were 9.6 million new TB cases and 1.5 million people died from this infectious disease (1). The widespread occurrence of drug-resistant (DR) TB (DR-TB), especially multidrug-resistant (MDR) TB (MDR-TB), has become a major global public health concern. In 2014, an estimated 3.3% of new TB cases and 20% of previously treated cases had MDR-TB and approximately 190,000 people died of MDR-TB worldwide. Treatment for MDR-TB is longer and requires more expensive and more toxic second-line drugs.Ethambutol (EMB) is an essential first-line drug for TB treatment and plays an important role in the chemotherapy of DR-TB (2). EMB acts against M. tuberculosis through inhibiting the membrane-associated arabinosyl transferases that are encoded by the embCAB operon (which included three contiguous genes, embC, embA, and embB) and that participate in the synthesis of arabinogalactan and lipoarabinomannan of the mycobacterial cell wall (3, 4). Mutations in the embCAB operon have been identified to confer resistance to EMB (3), with embB codon 306 (embB306) b...

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Characterization of Mycobacterium tuberculosis isolates from Hebei, China: genotypes and drug susceptibility phenotypes

Cited by 27 publications

References 28 publications

The study on the association between Beijing genotype family and drug susceptibility phenotypes of Mycobacterium tuberculosis in Beijing

The study on the association between Beijing genotype family and drug susceptibility phenotypes of Mycobacterium tuberculosis in Beijing

Resistance of Mycobacterium tuberculosis strains to Rifampicin: A systematic review and meta-analysis

Association between embB Codon 306 Mutations, Phenotypic Resistance Profiles, and Genotypic Characterization in Clinical Mycobacterium tuberculosis Isolates from Hebei, China

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