2016
DOI: 10.1128/aac.00532-16
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Association between embB Codon 306 Mutations, Phenotypic Resistance Profiles, and Genotypic Characterization in Clinical Mycobacterium tuberculosis Isolates from Hebei, China

Abstract: b Ethambutol (EMB) is an essential first-line drug for tuberculosis (TB) treatment. Nucleotide substitutions at embB codon 306 (embB306) have been proposed to be a potential marker for EMB resistance and a predictor of broad drug resistance in clinical Mycobacterium tuberculosis isolates. However, discordant findings about the association between embB306 mutations and EMB resistance were reported. Hebei Province is located in the Beijing-Tianjin-Hebei integration region in China; however, little information ab… Show more

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Cited by 7 publications
(7 citation statements)
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“…68 Although the causal relationship between embB and ubiA missense mutations with EMB resistance has been established, missense mutations at embB can only explain 40-68% of the global EMB resistance occurrence. 12,69 Moreover, the presence of EMBsusceptible clinical isolates with embB M306V or M306I missense mutations has been reported in multiple studies. [70][71][72] Such discrepancies might be due to inappropriate critical concentrations (CC; i.e.…”
Section: Cross-resistance Between Inh and Eth/pth Is Discussed In Secmentioning
confidence: 99%
See 1 more Smart Citation
“…68 Although the causal relationship between embB and ubiA missense mutations with EMB resistance has been established, missense mutations at embB can only explain 40-68% of the global EMB resistance occurrence. 12,69 Moreover, the presence of EMBsusceptible clinical isolates with embB M306V or M306I missense mutations has been reported in multiple studies. [70][71][72] Such discrepancies might be due to inappropriate critical concentrations (CC; i.e.…”
Section: Cross-resistance Between Inh and Eth/pth Is Discussed In Secmentioning
confidence: 99%
“…Whereas the occurrence of embB mutations among EMB‐resistant clinical isolates was consistent throughout the globe, occurrence of ubiA mutations in EMB‐resistant strains can vary according to the geographical region considered . Although the causal relationship between embB and ubiA missense mutations with EMB resistance has been established, missense mutations at embB can only explain 40–68% of the global EMB resistance occurrence . Moreover, the presence of EMB‐susceptible clinical isolates with embB M306V or M306I missense mutations has been reported in multiple studies .…”
Section: Mechanisms Of Drug Resistance In Mtbmentioning
confidence: 99%
“…Furthermore, certain mutations within the upstream region (UR) of embA are associated with EMB resistance (Cui et al, 2014;Brossier et al, 2015;Zhao et al, 2015). However, the presence of these mutations in embAB was also observed in EMBsusceptible isolates (Shi et al, 2011;Zhao et al, 2015;Li et al, 2016;Sun et al, 2018). Besides, there are no mutations within the particular genes responsible for EMB resistance in some EMB-resistant isolates, implying the involvement of other resistance mechanisms (Sharma and Bisht, 2017;Sharma et al, 2018).…”
Section: Introductionmentioning
confidence: 99%
“…Mutations in the embCAB operon (mainly embB) were most commonly associated with EMB resistance, with variations of codon embB 306, 406, and 497 as the hot spots for mutations (6)(7)(8)(9). However, information on the correlation between EMB resistance and embCAB mutations was scarce until now in China (9), with most studies investigating mutations only in embB (10)(11)(12)(13). Furthermore, studies often reported aggregate test performance containing sensitivity and specificity (8,9,11,12,14) and infrequently report on EMB MIC measurements.…”
mentioning
confidence: 99%
“…However, information on the correlation between EMB resistance and embCAB mutations was scarce until now in China (9), with most studies investigating mutations only in embB (10)(11)(12)(13). Furthermore, studies often reported aggregate test performance containing sensitivity and specificity (8,9,11,12,14) and infrequently report on EMB MIC measurements. To improve the accuracy of the molecular diagnostic, a detailed analysis of the differential effects of the embCAB mutations on EMB resistance is necessary.…”
mentioning
confidence: 99%