Characterization of Multiple Helicobacter bizzozeronii Isolates From a Finnish Patient With Severe Dyspeptic Symptoms and Chronic Active Gastritis

Abstract

Helicobacter bizzozeronii remains the only "H. heilmannii" species isolated from human gastric mucosa although it has been an infrequent observation among "H. heilmannii"-infected patients in PCR-based screening studies. The relevance of H. bizzozeronii and other potentially zoonotic gastric Helicobacter spp. in human disease remains to be determined.

Identification and Characterization of a Lipopolysaccharide ,2,3-Sialyltransferase from the Human Pathogen Helicobacter bizzozeronii

“…Escherichia coli strains were cultivated on Luria-Bertani (LB) medium or 2ϫ yeast extract-tryptone (2ϫYT) medium (Bio 101, Carlsbad, CA) supplemented with 150 mg/liter of ampicillin or 20 mg/liter of chloramphenicol when needed. DNA extraction was performed as described previously (30).…”

“…However, in gastric biopsy specimens of a minority of patients with upper gastrointestinal symptoms (0.17 to 2.3%), long, tightly coiled, spiral bacteria, ascribed to non-pylori Helicobacter spp., have been observed (5). Among the five non-pylori gastric Helicobacter species reported to be involved in human infections (19), only Helicobacter bizzozeronii has been successfully cultivated from human gastric biopsy specimens (27,30). Humans may acquire the infections as a consequence of direct contact with dogs or cats (35), which are normally colonized by H. bizzozeronii (21).…”

Helicobacter heilmannii sensu lato: An overview of the infection in humans

“…So far, very few laboratories have succeeded in the isolation of H. heilmannii s.l. from the gastric mucosa of cats, dogs, or pigs [15,44,45,67] and only H. bizzozeronii and H. felis have been isolated from the human gastric mucosa [35,[68][69][70] . Currently, the most accurate method available for conclusive species identification is the use of PCR, followed by sequencing of specific target genes.…”

Genome Sequence of Helicobacter bizzozeroniiStrain CIII-1, an Isolate from Human Gastric Mucosa

“…However, although virtually all cats and dogs carry this Helicobacter species, its pathogenic significance in these animals remains unknown (5). Here we present the genome sequence of the Finnish human H. bizzozeronii strain CIII-1, isolated in March 2008 from corpus biopsy tissue of a 45-year-old female patient with severe gastric symptoms (8). To our knowledge, this is the first genome sequence of nonpylori gastric Helicobacter isolated from humans.…”

“…However, in gastric biopsy tissues of a minority of patients (0.17 to 2.3%) with upper gastrointestinal symptoms, long, tightly coiled spiral bacteria, ascribed to nonpylori Helicobacter spp., can be observed (2). Among the five nonpylori gastric Helicobacter species reported to be associated with human infections (5), only Helicobacter bizzozeronii has been successfully cultivated from gastric biopsy tissues of human patients (7,8). Humans potentially acquire the infections as a consequence of direct contact with dogs and cats (10), which are normally colonized by H. bizzozeronii (6).…”