Characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and application in identification of antigenic variation in relation to vaccine strain selection

Yang, Ming; Xu, Wanhong; Goolia, Melissa; Zhang, Zhidong

doi:10.1186/1743-422x-11-136

Cited by 19 publications

(14 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A large polyprotein, encoded by the ORF, is subsequently cleaved by viral proteases (10) to produce four structural polypeptides (VP1-4), with VP1-3 being exposed on the surface of the virus, encapsulating the RNA genome. Of these, the VP1 protein (also referred to as 1D), encoded by, on average, 639 nt, contains several major antigenic determinants (11,12) in addition to playing a number of other critical roles, including viral attachment, cell entry and stimulation of immunity.…”

Section: Foot and Mouth Disease Virus: Genome Organisationmentioning

confidence: 99%

Genomics and outbreaks: foot and mouth disease

Freimanis¹,

Nardo²,

Bankowska³

et al. 2016

Rev. Sci. Tech. OIE

View full text Add to dashboard Cite

Foot and mouth disease virus (FMDV) is an animal pathogen of global economic significance. Identifying the sources of outbreaks plays an important role in disease control; however, this can be confounded by the ease with which FMDV can spread via movement of infected livestock and animal products, aerosols or fomites, e.g. contaminated persons and objects. As sequencing technologies have advanced, this review highlights the uses of viral genomic data in helping to understand the global distribution and transboundary movements of FMDV, and the role that these approaches have played in control and surveillance programmes. The recent application of next-generation sequencing platforms to address important epidemiological and evolutionary challenges is discussed with particular reference to the advent of 'omics' technologies.

show abstract

Section: Foot and Mouth Disease Virus: Genome Organisationmentioning

confidence: 99%

Genomics and outbreaks: foot and mouth disease

Freimanis¹,

Nardo²,

Bankowska³

et al. 2016

Rev. Sci. Tech. OIE

View full text Add to dashboard Cite

show abstract

“…In order to dissect the discrepancies of individual epitopes on the VLPs and unassembled proteins, a solution competitive ELISA was developed. Four neutralizing mAbs of FMDV, F21-48, F21-64, F21-58, and F21-41, used in the solution competitive ELISA were previously studied ( 17 ), which recognized antigenic site 1 on the G-H loop of VP1 at Amino Acid (aa) 148, and aa 136–151, antigenic site 2 in the region of VP2 at aa 77, and antigenic site 3 of VP1 at aa 43–44, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The binding abilities of the VLPs and the unassembled capsid proteins were analyzed by four neutralizing monoclonal antibodies (MAbs) of FMDV (F21-48, F21-64, F21-58, and F21-41) provided by Dr. Yang (National Centre for Foreign Animal Disease, Canada) ( 17 ).…”

Section: Methodsmentioning

confidence: 99%

The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O

Xiao

Zhang²,

He³

et al. 2021

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)–fused capsid proteins of FMDV serotype O by single plasmid in Escherichia coli was achieved with an optimal tandem permutation (VP0–VP3–VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine.

show abstract

“…Isolation of mAb‐resistant mutants was performed as previously described (Yang, Xu, Goolia, & Zhang, ). Briefly, SVDV (UK 27/72, tissue culture infective dose 10 7 /ml) was mixed with the purified mAb F44SVD–136 and incubated at 37°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Development and evaluation of swine vesicular disease isotype‐specific antibody ELISAs based on recombinant virus‐like particles

Yang

Gagliardi

McIntyre

et al. 2019

Transbound Emerg Dis

Self Cite

View full text Add to dashboard Cite

Swine vesicular disease (SVD) is a contagious viral disease of pigs. The clinical signs of SVD are indistinguishable from other vesicular diseases, such as senecavirus A infection (SVA) and foot‐and‐mouth disease (FMD). Rapid and accurate diagnostic tests of SVD are considered essential in countries free of vesicular diseases. Competitive ELISA (cELISA) is the serological test used routinely. However, although cELISA is the standard test for SVD antibody testing, this test produces a small number of false‐positive results, which caused problems in international trade. The current project developed a SVD isotype antibody ELISA using recombinant SVD virus‐like particles (VLP) and an SVD‐specific monoclonal antibody (mAb) to reduce the percentage of false positives. The diagnostic specificities of SVD‐VLP isotype ELISAs were 98.7% and 99.6% for IgM and IgG. The SVD isotype ELISAs were SVD‐specific, without cross‐reactivity to other vesicular diseases. A panel of 16 SVD‐positive reference sera was evaluated using the SVD‐VLP isotype ELISAs. All sera were correctly identified as positive by the two combined SVD‐VLP isotype ELISAs. Comparison of the test results showed a high level of correlation between the SVDV antigen isotype ELISAs and SVD‐VLP isotype ELISAs. 303 sera from animals lacking clinical signs and history of SVDV exposure were identified positive using SVD cELISA. These samples were examined using SVD‐VLP isotype ELISAs. Of the 303 serum samples, five were positive for IgM, and five of 303 were positive for IgG. Comparable to virus neutralization test results, SVD isotype ELISAs significantly reduced the false‐positive samples. Based on above test results, the combined use of cELISA and isotype ELISAs can reduce the number of false‐positive samples and the use of time‐consuming virus neutralization tests, with benefit for international trade in swine and related products.

show abstract

Characterization of monoclonal antibodies against foot-and-mouth disease virus serotype O and application in identification of antigenic variation in relation to vaccine strain selection

Cited by 19 publications

References 48 publications

Genomics and outbreaks: foot and mouth disease

Genomics and outbreaks: foot and mouth disease

The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O

Development and evaluation of swine vesicular disease isotype‐specific antibody ELISAs based on recombinant virus‐like particles

Contact Info

Product

Resources

About