Characterization of mitochondrial dicarboxylate/tricarboxylate transporters from grape berries

Regalado, Ana; Pierri, Ciro Leonardo; Bitetto, Maria; Laera, Valentina Liliana; Pimentel, Catarina; Francisco, Rita; Passarinho, J. A.; Chaves, M. M.; Agrimi, Gennaro

doi:10.1007/s00425-012-1786-8

Cited by 24 publications

(24 citation statements)

References 34 publications

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“…DTC’s can transport all the di- and tricarboxylates of the TCA cycle with the exception of fumarate, and they exhibit a high specificity for malate. The expression of two DTCs genes ( VvDTC2 and VvDTC3 ) correlated well with the malic acid content in grape berry mesocarp close to the onset of ripening, and might be involved in the transport of malate into mitochondria [137]. In response to heat treatment, it was found that a large number of DTC isogenes, ( VIT_00s0607g00010, VIT_00s0827g00020, VIT_00s0827g00030, VIT_07s0031g02470, VIT_08s0007g07270 ; cluster 4; annotated mitochondrial 2-oxaglutarate/malate carrier protein in RefSeq [54]) were induced especially at VS were malic acid respiration occurs at maximal rate, thus implying their putative role in malic acid metabolism.…”

Section: Resultsmentioning

confidence: 99%

Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

et al. 2014

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BackgroundGlobal climate change will noticeably affect plant vegetative and reproductive development. The recent increase in temperatures has already impacted yields and composition of berries in many grapevine-growing regions. Physiological processes underlying temperature response and tolerance of the grapevine fruit have not been extensively investigated. To date, all studies investigating the molecular regulation of fleshly fruit response to abiotic stress were only conducted during the day, overlooking possible critical night-specific variations. The present study explores the night and day transcriptomic response of grapevine fruit to heat stress at several developmental stages. Short heat stresses (2 h) were applied at day and night to vines bearing clusters sequentially ordered according to the developmental stages along their vertical axes. The recently proposed microvine model (DRCF-Dwarf Rapid Cycling and Continuous Flowering) was grown in climatic chambers in order to circumvent common constraints and biases inevitable in field experiments with perennial macrovines. Post-véraison berry heterogeneity within clusters was avoided by constituting homogenous batches following organic acids and sugars measurements of individual berries. A whole genome transcriptomic approach was subsequently conducted using NimbleGen 090818 Vitis 12X (30 K) microarrays.ResultsPresent work reveals significant differences in heat stress responsive pathways according to day or night treatment, in particular regarding genes associated with acidity and phenylpropanoid metabolism. Precise distinction of ripening stages led to stage-specific detection of malic acid and anthocyanin-related transcripts modulated by heat stress. Important changes in cell wall modification related processes as well as indications for heat-induced delay of ripening and sugar accumulation were observed at véraison, an effect that was reversed at later stages.ConclusionsThis first day - night study on heat stress adaption of the grapevine berry shows that the transcriptome of fleshy fruits is differentially affected by abiotic stress at night. The present results emphasize the necessity of including different developmental stages and especially several daytime points in transcriptomic studies.

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Section: Resultsmentioning

confidence: 99%

Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

et al. 2014

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“…The half‐saturation constant of CiC for citrate at pH 7 is in the range of 0.062–0.13 mM , whereas the citrate K m for yeast CiC1 and CiC2 are 0.36 and 0.16 mM, respectively . The range of DTC for citrate measured at pH 7 is 0.65–1.91 mM and the lower values have been obtained at pH 6 in citrate/citrate exchange in A. thaliana and N. tabacum (0.15–0.31 mM) .…”

Section: Identification and Characterization Of Cics And Dtcsmentioning

confidence: 86%

“…The maize carrier incorporated into liposomes is able to exchange citrate against citrate, malate, succinate, malonate, and isocitrate as well as differently to CiC animals, oxoglutarate, and oxaloacetate while it is not able to transport phosphoenolpyruvate. In the postgenomic era in Arabidopsis thaliana , Nicotiana tabacum , and Vitis vinifera , a mitochondrial DTC has been identified by overexpression in Escherichia coli . Like maize CiC, the DTCs are capable of transporting both dicarboxylates (such as oxoglutarate, oxaloacetate, malate, succinate, maleate, malonate, and oxoadipate) and tricarboxylates (such as citrate, isocitrate, cis ‐aconitate, and trans ‐aconitate) but not phosphoenolpyruvate.…”

Section: Identification and Characterization Of Cics And Dtcsmentioning

confidence: 99%

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Mitochondrial tricarboxylate and dicarboxylate–Tricarboxylate carriers: from animals to plants

2014

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The citrate carrier (CiC), characteristic of animals, and the dicarboxylate-tricarboxylate carrier (DTC), characteristic of plants and protozoa, belong to the mitochondrial carrier protein family whose members are responsible for the exchange of metabolites, cofactors, and nucleotides between the cytoplasm and the mitochondrial matrix. Most of the functional data on these transporters are obtained from the studies performed with the protein purified from rat, eel yeast, and maize mitochondria or recombinant proteins from different sources incorporated into phospholipid vesicles (liposomes). The functional data indicate that CiC is responsible for the efflux of acetyl-CoA from the mitochondria to the cytosol in the form of citrate, the primer for fatty acid, cholesterol synthesis, and histone acetylation. Like the CiC, the citrate exported by DTC from the mitochondria to the cytosol in exchange for oxaloacetate can be cleaved by citrate lyase to acetyl-CoA and oxaloacetate and used for fatty acid elongation and isoprenoid synthesis. In addition to its role in fatty acid synthesis, CiC is involved in other processes such as gluconeogenesis, insulin secretion, inflammation, and cancer progression, whereas DTC is involved in the production of glycerate, nitrogen assimilation, ripening of fruits, ATP synthesis, and sustaining of respiratory flux in fruit cells. This review provides an assessment of the current understanding of CiC and DTC structural and biochemical characteristics, underlying the structure-function relationship of these carriers. Furthermore, a phylogenetic relationship between CiC and DTC is proposed. V C 2014 IUBMB Life, 66(7): [462][463][464][465][466][467][468][469][470][471] 2014

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“…This requires clarity about the mitochondrial carrier proteins that transport GABA, malate, and pyruvate. Although information regarding the responses of these carrier proteins to salinity stress is still limited, recent discoveries of new carrier proteins and their responses to other stresses might be helpful in gaining some information on the role of these proteins in salinity stress adaptation (Lee & Millar, ; Li, Wang, Ma, & Zhang, ; Michaeli et al, ; Regalado et al, ).…”

Section: Mitochondrial Membrane Transport Proteinsmentioning

confidence: 99%

Connecting salt stress signalling pathways with salinity‐induced changes in mitochondrial metabolic processes in C3 plants

Che-Othman

Millar

Taylor

2017

Plant Cell & Environment

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Salinity exerts a severe detrimental effect on crop yields globally. Growth of plants in saline soils results in physiological stress, which disrupts the essential biochemical processes of respiration, photosynthesis, and transpiration. Understanding the molecular responses of plants exposed to salinity stress can inform future strategies to reduce agricultural losses due to salinity; however, it is imperative that signalling and functional response processes are connected to tailor these strategies. Previous research has revealed the important role that plant mitochondria play in the salinity response of plants. Review of this literature shows that 2 biochemical processes required for respiratory function are affected under salinity stress: the tricarboxylic acid cycle and the transport of metabolites across the inner mitochondrial membrane. However, the mechanisms by which components of these processes are affected or react to salinity stress are still far from understood. Here, we examine recent findings on the signal transduction pathways that lead to adaptive responses of plants to salinity and discuss how they can be involved in and be affected by modulation of the machinery of energy metabolism with attention to the role of the tricarboxylic acid cycle enzymes and mitochondrial membrane transporters in this process. Saline landscapes occur naturally, and many halophytes (salt tolerant plants) survive and complete their life cycle in these environments (Flowers & Colmer, 2015). Human activity including intense irrigation and land clearing can also cause soil salinity, as these activities lead to changes in the soil water table, which can contain high salt concentrations (Rengasamy, 2006). These agricultural practices result in a rising water table, which liberates soil solutes bringing them to the surface, creating salinized areas in previously productive land (Rengasamy, 2006). This severely limits crop production in these areas as typically elite high yielding crop varieties cannot tolerate these high salt concentrations (Roy, Negrão, & Tester, 2014).Although the salinization of soils has and is increasingly limiting crop production in many areas of the world (Sha Valli Khan, Nagamallaiah, Dhanunjay, Sergeant, & Hausman, 2014), reliable measures of salt-affected land are very difficult to obtain because salinity varies both spatially and temporally. Nevertheless, a number of studies have attempted to estimate the extent of soil salinity.According to the Food and Agriculture Organisation of the United Nations, it is estimated that approximately 20% of all global irrigated land has become salt-affected and this percentage is expected to continue to increase over time (FAO, 2009;Parihar, Singh, Singh, Singh, & Prasad, 2014). It was further estimated that this salinization of irrigated --------------------------------------------------This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium...

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Characterization of mitochondrial dicarboxylate/tricarboxylate transporters from grape berries

Cited by 24 publications

References 34 publications

Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

Day and night heat stress trigger different transcriptomic responses in green and ripening grapevine (vitis vinifera) fruit

Mitochondrial tricarboxylate and dicarboxylate–Tricarboxylate carriers: from animals to plants

Connecting salt stress signalling pathways with salinity‐induced changes in mitochondrial metabolic processes in C3 plants

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