Characterization of Miniature Probes for Cryosurgery, Thermal Ablation, and Irreversible Electroporation on Small Animals

Ranjbartehrani, Pegah; Etheridge, Michael L.; Ramadhyani, S.; Natesan, Harishankar; Bischof, John C.; Shao, Qi

doi:10.1002/adtp.202100212

Cited by 5 publications

(5 citation statements)

References 39 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mice bearing an MC-38 tumor on the hind limb were humanely euthanized, in accordance with protocols approved by the Institutional Animal Care and Use Committee (IACUC), University of Minnesota . Post-mortem tumor-bearing mice were used to test the feasibility of heating to the hyperthermic range for 15 min in tissue.…”

Section: Methodsmentioning

confidence: 99%

Injectable and Repeatable Inductive Heating of Iron Oxide Nanoparticle-Enhanced “PHIL” Embolic toward Tumor Treatment

Pasek-Allen

Kantesaria

Gangwar

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Deep-seated tumors of the liver, brain, and other organ systems often recur after initial surgical, chemotherapeutic, radiation, or focal treatments. Repeating these treatments is often invasive and traumatic. We propose an iron oxide nanoparticle (IONP)-enhanced precipitating hydrophobic injectable liquid (PHIL, MicroVention inc.) embolic as a localized dual treatment implant for nutrient deprivation and multiple repeatable thermal ablation. Following a single injection, multiple thermal treatments can be repeated as needed, based on monitoring of tumor growth/recurrence. Herein we show the ability to create an injectable stable PHIL-IONP solution, monitor deposition of the PHIL-IONP precipitate dispersion by μCT, and gauge the IONP distribution within the embolic by magnetic resonance imaging. Once precipitated, the implant could be heated to reach therapeutic temperatures >8 °C for thermal ablation (clinical temperature of ∼45 °C), in a model disk and a 3D tumor bed model. Heat output was not affected by physiological conditions, multiple heating sessions, or heating at intervals over a 1 month duration. Further, in ex vivo mice hind-limb tumors, we could noninvasively heat the embolic to an “ablative” temperature elevation of 17 °C (clinically 54 °C) in the first 5 min and maintain the temperature rise over +8 °C (clinically a temperature of 45 °C) for longer than 15 min.

show abstract

Section: Methodsmentioning

confidence: 99%

Injectable and Repeatable Inductive Heating of Iron Oxide Nanoparticle-Enhanced “PHIL” Embolic toward Tumor Treatment

Pasek-Allen

Kantesaria

Gangwar

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Preclinical comparison among different modes of FT is also still lacking. Another factor that further complicates the issue is that the device and setup of FTs vary among different laboratories: some use a clinical-size probe which is less than ideal for sub-centimeter tumors, while some groups opt to build probes specially for small animal research ( 312 ). When FT probes for small animals are used, they are usually not fully characterized: lacking an assessment of the ex vivo and in vivo physical performance of the probes with regards to the biological sequelae of tumor destruction.…”

Section: Challenges For Ft and Icimentioning

confidence: 99%

Combining energy-based focal ablation and immune checkpoint inhibitors: preclinical research and clinical trials

2023

Self Cite

View full text Add to dashboard Cite

Energy-based focal therapy (FT) uses targeted, minimally invasive procedures to destroy tumors while preserving normal tissue and function. There is strong emerging interest in understanding how systemic immunity against the tumor can occur with cancer immunotherapy, most notably immune checkpoint inhibitors (ICI). The motivation for combining FT and ICI in cancer management relies on the synergy between the two different therapies: FT complements ICI by reducing tumor burden, increasing objective response rate, and reducing side effects of ICI; ICI supplements FT by reducing local recurrence, controlling distal metastases, and providing long-term protection. This combinatorial strategy has shown promising results in preclinical study (since 2004) and the clinical trials (since 2011). Understanding the synergy calls for understanding the physics and biology behind the two different therapies with distinctive mechanisms of action. In this review, we introduce different types of energy-based FT by covering the biophysics of tissue-energy interaction and present the immunomodulatory properties of FT. We discuss the basis of cancer immunotherapy with the emphasis on ICI. We examine the approaches researchers have been using and the results from both preclinical models and clinical trials from our exhaustive literature research. Finally, the challenges of the combinatory strategy and opportunities of future research is discussed extensively.

show abstract

“…Therefore, high thermal conductivity of the material chosen for an applicator plays significant role. Due to this, common applicators are made of metals [38][39][40][41]. At the same time, sapphire features high thermal conductivity at cryogenic temperatures, that along with the mentioned properties makes it a favorable material for cryosurgery [42].…”

Section: Introductionmentioning

confidence: 99%

Manufacturing of Sapphire Crystals with Variable Shapes for Cryosurgical Applications

Dolganova,

Zotov,

Rossolenko

et al. 2024

Crystals

View full text Add to dashboard Cite

Consideration of sapphire shaped crystals as the material for manufacturing of medical instruments expands the opportunities of various approaches for diagnostics, exposure and treatment. Due to physical, mechanical and chemical properties of sapphire, as well as to its complex shape, such instruments are capable to demonstrate better performance for medical applications comparing to common tools. However, the manufacturing of high quality sapphire crystal with such geometry is still a complex issue, that usually requires application of various crystal growth techniques assisted with the automated weight control system. In this work, we consider one of such cases, that is the growth of a sapphire crystal, which can be applied for cryosurgery as an applicator due to a hollow-monolithic shape transition. Its hollow part can be filled with coolant in order to enable fast freezing of biological tissue during application. For this aim, it is of high importance to exclude the appearance of inclusions during the shape transition. To overcome this problem, we suggest using of noncapillary shaping (NCS) technique of crystal growth and study the weight signal measured during the manufacturing. We obtain the analytical description of the weight signal alteration that can be used as the program equation to control the crystal shape. We experimentally demonstrate the advantage of using such crystal for cryosurgery and obtaining faster ice-ball formation inside the model gelatin-based medium in comparison with the usage of the monolithic sapphire applicator of the same diameter. The demonstrated ability can be applied for future development of cryosurgical tools, while the analytical description of the weight signal could find its application for NCS manufacturing of sapphire crystals for other purposes.

show abstract

Characterization of Miniature Probes for Cryosurgery, Thermal Ablation, and Irreversible Electroporation on Small Animals

Cited by 5 publications

References 39 publications

Injectable and Repeatable Inductive Heating of Iron Oxide Nanoparticle-Enhanced “PHIL” Embolic toward Tumor Treatment

Injectable and Repeatable Inductive Heating of Iron Oxide Nanoparticle-Enhanced “PHIL” Embolic toward Tumor Treatment

Combining energy-based focal ablation and immune checkpoint inhibitors: preclinical research and clinical trials

Manufacturing of Sapphire Crystals with Variable Shapes for Cryosurgical Applications

Contact Info

Product

Resources

About