Characterization of microRNA expression in serous ovarian carcinoma

Abstract

Serous ovarian cancer is a major gynecologic malignancy with a poor 5‑year survival rate. However, little is known regarding the behavior and genetics of ovarian tumorigenesis. MicroRNAs (miRNAs) have been shown to be dysregulated in ovarian carcinomas. To assess the miRNA expression profiles in serous ovarian cancer, we defined the patterns of miRNA expression in 100 formalin‑fixed, paraffin‑embedded ovarian cancer tissues blocks as well as 50 corresponding normal oviduct tissues using miRNA microarray. MiRNA expression profiling showed that 63 miRNAs were downregulated and 43 miRNAs were upregulated in serous ovarian cancer tissues compared with control tissues. The expression of five dysregulated miRNAs was validated using quantitative polymerase chain reaction (RT‑qPCR). GO term and pathway analysis revealed that the biological process of the cell cycle was significantly enriched and the MAPK signaling pathway was highly involved in the progression of ovarian cancer. The results suggested that the aberrant expression of miRNAs is involved in ovarian carcinogenesis and thus these miRNAs may function as diagnostic and prognostic biomarkers.

Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines

“…Previous studies also demonstrated that miR-21 was abnormally highly expressed in ovarian cancer tissues, and was closely associated with the differentiation of tumor cells and lymph node metastasis, suggesting that miR-21 may promote the development of ovarian cancer as an oncogene (11). These studies indicated that the occurrence and development processes of ovarian cancer were closely associated with miRNA levels (11,12).…”

“…A number of studies have demonstrated that miRNAs are involved in the occurrence and development of ovarian cancer (10)(11)(12). Zhang et al (16) reported that miRNA was involved in the occurrence and development of ovarian cancer drug resistance by studying a stem cell-like epithelial ovarian cancer line.…”

Screening of potentially crucial genes and regulatory factors involved in epithelial ovarian cancer using microarray analysis

“…In the present study, PCDH9 was also found to be regulated by miR-92b-3p and miR-137. miR-92b-3p and miR-137 have been reported to exhibit altered expression levels in ovarian tumor cells (52,53). Taken together, ALDH1A2 and PCDH9 may be important in the progression of EOC through the regulation of TP53 or miR-92b-3p and miR-137.…”

The prognostic role of microRNA in epithelial ovarian cancer: a systematic review of literature with an overall survival meta-analysis

“…While it is believed to suppress metastasis, most studies done on the family show that it is overexpressed in ovarian cancer [9]. However, some studies have reported that miR-200 family members are either downregulated [9] or unchanged [10]. These conflicting results may be due to different normal controls or the inclusion of ovarian stromal cells which lack miR-expression.…”