Characterization of microglial attachment and cytokine release on biomaterials of differing surface chemistry

Leung, Braden K; Biran, Roy; Underwood, Clay J.; Tresco, Patrick A.

doi:10.1016/j.biomaterials.2008.03.045

Cited by 83 publications

(89 citation statements)

References 80 publications

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“…The supernatant enriched with microglia was collected and centrifuged (200g, 5 min). Microglial cell culture purity was quantified after immunolabelling using CD11b antibody (1 : 200; Abcam, Belgium) and found to be above 90% (see details in the electronic supplementary material) in accordance with previous reports that used a similar isolation technique [18,28]. A total of 6 Â 10 4 viable cells ml 21 were seeded on P(TMC-CL) substrates secured at the bottom of a 24-well plate with a silicone o-ring (Epidor, Spain) and cultured in DMEM/F12 medium (Gibco) supplemented with 10% FBS and 1% P/S for 1 or 5 days.…”

supporting

confidence: 77%

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The role of the surface on microglia function: implications for central nervous system tissue engineering

Pires

Rocha

Ambrosio

et al. 2015

J. R. Soc. Interface.

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In tissue engineering, it is well accepted that a scaffold surface has a decisive impact on cell behaviour. Here we focused on microglia-the resident immune cells of the central nervous system (CNS)-and on their response to poly(trimethylene carbonate-co-1-caprolactone) (P(TMC-CL)) fibrous and flat surfaces obtained by electrospinning and solvent cast, respectively. This study aims to provide cues for the design of instructive surfaces that can contribute to the challenging process of CNS regeneration. Cell morphology was evidently affected by the substrate, mirroring the surface main features. Cells cultured on flat substrates presented a round shape, while cells with elongated processes were observed on the electrospun fibres. A higher concentration of the pro-inflammatory cytokine tumour necrosis factor-a was detected in culture media from microglia on fibres. Still, astrogliosis is not exacerbated when astrocytes are cultured in the presence of microgliaconditioned media obtained from cultures in contact with either substrate. Furthermore, a significant percentage of microglia was found to participate in the process of myelin phagocytosis, with the formation of multinucleated giant cells being observed only on films. Altogether, the results presented suggest that microglia in contact with the tested substrates may contribute to the regeneration process, putting forward P(TMC-CL) substrates as supporting matrices for nerve regeneration.

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supporting

confidence: 77%

“…The chemistry of the surface was found to influence the cytokine release profile of microglia depending on its hydrophobicity [18]. In what concerns surface topography, the effect of nanostructured silicone or poly(dimethylsiloxane) surfaces on microglia morphology, adhesion [19,20] or motility [19] was also investigated.…”

Section: Introductionmentioning

confidence: 99%

The role of the surface on microglia function: implications for central nervous system tissue engineering

Pires

Rocha

Ambrosio

et al. 2015

J. R. Soc. Interface.

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“…Thus the functionality is limited to short periods (weeks to months) and continued signal deterioration. Causes of such electrode failure include poor bio-abio interface, tissue damage by probe micromotion within the soft nerve tissue, and electrode insulation as a result of tissue scar formation [6,7]. These limitations require neurointerface recalibration prior to each recording session and the gradual increase of the amount of electrical stimulation needed to maintain neural responses over time, which in turn raises the risk of electrolytic tissue damage and further compromises the long-term stability of the neurointerface.…”

Section: Issues In Neurointerfacesmentioning

confidence: 99%

Carbon nanotube coated high-throughput neurointerfaces in assistive environments

Romero-Ortega

Butt

Iqbal

2009

Proceedings of the 2nd International Conference on PErvasive Technologies Related to Assistive Environments

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Loosing motor activity due to impaired or damaged nerves or muscles affects millions of people world-wide. The resulting lack of mobility and/or impaired communication bears enormous personal, economical and social costs. While several assistive technologies exist, they rely on device surrogates to compensate for the lack of movement and thus provide limited utility and unnatural interface with the user. The ability of interfacing populations of neurons with super high-density multielectrode arrays (SD-MEA) can provide the sensing from and control of bionics devices by thought. Here we propose a neurointerfacing approach using SD-MEAs coated with carbon nanotubes and high-speed computing to overcome latency and long-term electrical viability bottlenecks that are essential in assistive environments. The proposed approach provides ability for fast integration of recording/stimulation from thousands of individually addressable electrodes, while coordinating a real-time computing approach to register, recognize, analyze and respond appropriately to the biological signals from the motor neurons and sensory signals from the robotic prosthesis.

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“…Histological analyses following cortical implant insertion have shown encapsulation of the implant by activated microglia and astrocytes in a process known as glial scarring [3]. Evidence suggests that microglia mediate this response by becoming activated upon contact with many of the materials used in current implanted electrodes [8]. Improving neuron-electrode connectivity by reducing microglial activation and the subsequent scarring around neural implants has therefore become an important area of BCI research.…”

Section: Introductionmentioning

confidence: 99%

“…These materials are known for their ability to resist protein adsorption and microglial attachment, factors thought to be important in initiating the foreign body response [8,[35][36][37][38]. PEGylated, micro-patterned DLC substrates are used to spatially control the adhesion of inflammatory cells (principally myeloid cells such as macrophages and CNS microglia).…”

Section: Introductionmentioning

confidence: 99%

Spatially Controlling Neuronal Adhesion and Inflammatory Reactions on Implantable Diamond

Regan

Taylor

Uney

et al. 2011

IEEE J. Emerg. Sel. Topics Circuits Syst.

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Characterization of microglial attachment and cytokine release on biomaterials of differing surface chemistry

Cited by 83 publications

References 80 publications

The role of the surface on microglia function: implications for central nervous system tissue engineering

The role of the surface on microglia function: implications for central nervous system tissue engineering

Carbon nanotube coated high-throughput neurointerfaces in assistive environments

Spatially Controlling Neuronal Adhesion and Inflammatory Reactions on Implantable Diamond

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