Characterization of Microbial Communities, Identification of Cr(VI) Reducing Bacteria in Constructed Wetland and Cr(VI) Removal Ability of Bacillus cereus

Lin, Hua; You, Shijun; Liu, Liheng

doi:10.1038/s41598-019-49333-4

Cited by 16 publications

(9 citation statements)

References 36 publications

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“…Similarly, Lin et al performed a characterization of microbial communities in different wetland substrates to treat Cr (VI)-contaminated water using molecular techniques such as DGGE and sequencing. This research used band analysis and diversity indices to establish that under the stress of Cr (VI), bacterial diversity decreases significantly, and sensitive bacteria tend to die and become replaced by bacteria that are tolerant or sensitive to this metal [29].…”

Section: Discussionmentioning

confidence: 99%

Molecular techniques for the assessment of Cr (VI) reduction by Bacillus thuringiensis

et al. 2021

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Effluent pollution with Cr (VI) is a worldwide environmental problem. In the Pasto River (southeastern, Colombia), previous studies reported contamination with this metal at points near tanneries. To establish the role of Bacillus thuringiensis in Cr (VI) reduction in water from Pasto River, experiments were carried out with untreated Pasto River water (treatment 1), sterile Pasto River water inoculated with B. thuringiensis (treatment 2), and unsterilized Pasto River water inoculated with B. thuringiensis (treatment 3). All experiments were conducted in bioreactors with a controlled temperature of 20 °C and constant agitation for 156 h. Samples of 20 mL were taken every 12 h from each treatment to track Cr (VI) reduction levels and to confirm microorganism identity via molecular methods involving denaturing gradient gel electrophoresis (DGGE), restriction enzyme digestion profiles (RFLP), and bioinformatic analysis. Cr (VI) reduction was higher in treatment 3 (99:42 %) as opposed to treatment 2 (76:12 %) and treatment 1 (74:46 %). The molecular identity of B. thuringiensis was determined via sequencing of the 16SrRNA gene, and RFLP assessments in all three treatments revealed B. thuringiensis profiles. Since B. thuringiensis was present in all three treatments trough time, Cr (VI) reduction can be attributed to this bacterium.

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Section: Discussionmentioning

confidence: 99%

Molecular techniques for the assessment of Cr (VI) reduction by Bacillus thuringiensis

et al. 2021

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“…It is also observed that the decrease of the Chromium amount in water occurs faster than in the case of Nickel, probably determined by fast reduction of Cr (VI) to Cr (III). As reported by Ashraf et al 8 and Lin et al 9 , this process is mainly carried out by microorganisms and soil organic matter and makes chromium a less mobile species than nickel (II).…”

Section: Nickel (Ii) and Total Chromium Removal Kinetics In The Horiz...mentioning

confidence: 93%

Nickel and chromium removal efficiency using a horizontal subsurface wetland

Hernández

Villar

2022

Afinidad

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This study assessed nickel and total chromium removal using a horizontal subsurface flow constructed wetland. Analysis of these metals concentration in Cyperus Alternifolius plant was carried out at first. Subsequently, removal kinetics of contaminants in the wetland were evaluated, obtaining a better fit to the Monod model for both metals. Determination of nickel and total chromium content in the effluent from the National Domestic Household Producer Industry (INPUD “1ro de Mayo”) was also carried out and removal of the employed metals in the wetland was evaluated. The removal efficiency obtained for these metals using standard solutions and the INPUD wastewater was above 98%. Greater retention of metals was obtained in the plant, mainly in roots and leaves evidencing the importance of this component in metal elimination process. It was also observed a predominance of nickel accumulation in the leaves, presenting a high bioavailability.

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“…And Arthrobacter sp. (Shimei et al, 2021), (Pseudomonas Alcaliphila Strain NewG-2) (Noura et al, 2020), (Pseudomonas Strain CPSB21) (Pratishtha et al, Bacillus cereus (Bacillus cereus) (Lin et al, 2019). They can alleviate Cr stress by their own stress mechanism and promote plant growth.…”

Section: Introductionmentioning

confidence: 99%

“…They can alleviate Cr stress by their own stress mechanism and promote plant growth. Furthermore, the removal rate of Cr 6+ by Bacillus cereus was close to 100% when pH=7, the temperature is 35℃ (Lin et al, 2019). Therefore, under the background of extensive research on rhizosphere microorganisms, it is of great signi cance to explore response of rhizosphere microbial community to chromium stress, as well as the protection of microorganisms on plants.…”

Section: Introductionmentioning

confidence: 99%

Effects of Cr stress on bacterial community structure and composition in rhizosphere soils of Iris tectorum

Wang¹,

Yang²,

Wang³

et al. 2022

Preprint

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Heavy metal Cr pollution caused serious stress to soil bacterial community. However, the joint effects of Cr stress and different cultivation patterns on bacterial communities of rhizosphere soils remain unclear. In this study, Iris tectorum was selected as the research object, and combined with 16S rRNA high-throughput sequencing technology, the effects of high Cr levels and different cultivation patterns on the structure and diversity of rhizosphere soil bacterial community of I. tectorum were analyzed. The results showed that iris had strong tolerance and enrichment to Cr. However, under Cr stress, the diversity and abundance index of rhizosphere bacteria decreased by 8.5% and 6.8% on average, and Sobs index decreased by 7.6%. Moreover, bacterial community changed by 20.1% due to the addition of Cr, further leading to a 15.9% decrease in the common species of the bacterial community, among which Proteobacteria, Actinobacteriota, Chloroflexi and Acidobacteriota accounted for more than 74.8% of the total sequence. The abundance of bacterial community was significantly improved under two-cultivated pattern and three-cultivated pattern. Moreover, the two-cultivated pattern significantly enhanced the synergistic effect among the dominant species, thus improving the rhizosphere soil microenvironment. In conclusion, this study revealed that different cultivation patterns can change the composition and symbiotic relationship of rhizosphere bacterial communities of iris, thus alleviating the negative effects of Cr stress on diversity and abundance. These results provided a reference for understanding the response of rhizosphere bacterial communities under Cr stress under different cultivation modes.

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Characterization of Microbial Communities, Identification of Cr(VI) Reducing Bacteria in Constructed Wetland and Cr(VI) Removal Ability of Bacillus cereus

Cited by 16 publications

References 36 publications

Molecular techniques for the assessment of Cr (VI) reduction by Bacillus thuringiensis

Molecular techniques for the assessment of Cr (VI) reduction by Bacillus thuringiensis

Nickel and chromium removal efficiency using a horizontal subsurface wetland

Effects of Cr stress on bacterial community structure and composition in rhizosphere soils of Iris tectorum

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