2009
DOI: 10.1007/s00418-009-0629-6
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Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers

Abstract: Bone marrow-derived mesenchymal stem cells (BM-MSCs) can differentiate into many lineages. Although the growing interest in BM-MSCs has led to a number of characterization studies, some important biochemical and immunohistochemical properties are still lacking. In this study, morphological and immunophenotypic properties of BM-MSCs were examined in detail. Differentiation potential and growth kinetics of adult rat BM-MSCs were also determined. Immunohistochemistry and RT-PCR results indicated that BM-MSCs expr… Show more

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Cited by 183 publications
(163 citation statements)
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References 19 publications
(28 reference statements)
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“…Isolation and culture of rBM-MSCs were performed as previously described (9). Under sterile conditions, both rat femur and tibiae were excised, and the bone marrow was flushed with MEM-Earle medium supplemented with 15% FBS, 1% Penicillin/Streptomycin (Gibco Invitrogen, Life Technologies, Paisley, UK).…”
Section: Isolation and Culture Of Rbm-mscsmentioning
confidence: 99%
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“…Isolation and culture of rBM-MSCs were performed as previously described (9). Under sterile conditions, both rat femur and tibiae were excised, and the bone marrow was flushed with MEM-Earle medium supplemented with 15% FBS, 1% Penicillin/Streptomycin (Gibco Invitrogen, Life Technologies, Paisley, UK).…”
Section: Isolation and Culture Of Rbm-mscsmentioning
confidence: 99%
“…The immunohistochemistry studies on the P 3 cells were performed as previously described (9), and the list of primary antibodies is given in Table I. Samples were fixed in ice-cold methanol for 10 min and treated with 0.025% Triton X-100 (Merck, Darmstadt, Germany) for permeabilization.…”
Section: Immunostainingsmentioning
confidence: 99%
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“…In our previous report, bone marrow-derived MSCs (BMMSCs) were shown to be involved in successful functional regeneration following experimental SCI (14). Similarly, rat adipose tissue-derived mesenchymal stem cells (rAT-MSCs) were shown to express the early neurogenic markers GFAP, Nestin and c-Fos, indicating that the steps toward neurogenic differentiation occurred in chemical cocktail-supplemented media (4).…”
Section: █ Introductionmentioning
confidence: 96%
“…The result is of utmost importance in stem cell researches. ASCs with stem cell properties have previously been identified in the bone marrow mesenchyme, skin, hair, dental pulp, kidney, and even peripheral blood (Karaoz et al, 2009). However, adult cells with stem cell properties are safer and have greater practical use, having been employed for over 50 years to replace bone marrow in leukemia and non-hematological diseases without causing malignant transformation (Klopp et al, 2012).During the last few year's isolations of AMSCs from different sources have been reported.…”
Section: Introductionmentioning
confidence: 99%