Characterization of mean stem density, fibre length and lignin from two Acacia species and their hybrid

Muhammad, Asif Javed; Ong, Seong Siang; Ratnam, R Wickneswari V

doi:10.1007/s11676-017-0465-9

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…Acacia is an economically important genus, belongs to family Fabaceae . There are 1380 species of Acacia and with about two third are native to Australia while others are found in tropical and subtropical regions of the World ( Maslin et al, 2003 , Orchard and Maslin, 2003 ; Muhammad et al, 2017 , Muhammad et al, 2018 ). Acacia species are economically very important and well adopted to arid conditions and in agroforestry.…”

Section: Introductionmentioning

confidence: 99%

Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers

Ismail

Ahmad

Nadeem

et al. 2020

Saudi Journal of Biological Sciences

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Acacia species are very important tree species in tropical and subtropical countries of the World for their economic and medicinal benefits. Precise identification of Acacia is very important to distinguish the invasive species from rare species however, it is difficult to differentiate Acacia species based on morphological charcters. In addition, precise identification is also important for wood charcterization in the forest industry as these species are declining due to illegal logging and deforestation. To overcome thsese limitations of morphological identification, DNA barcoding is being used as an efficient and quick approach for precise identification of tree species. In this study, we selected two chloroplast and plastid base DNA markers ( rbcL and matK ) for the identification of five selected tree species of Acacia (A. albida, A. ampliceps, A. catechu, A. coriacea and A. tortilis) . The genomic DNA of the selected Acacia species was extracted, amplified through PCR using specific primers and subsequently sequenced through Sanger sequencing. In matK DNA marker the average AT nucleotide contents were higher (59.46%) and GC contents were lower (40.44%) as compared to the AT (55.40%) and GC content (44.54%) in rbcL marker. The means genetic distance K2P between the Acacia species was higher in matK (0.704%) as compared to rbcL (0.230%). All Acacia species could be identified based on unique SNPs profile. Based on SNP data profiles, DNA sequence based scannable QR codes were developed for accurate identification of Acacia species. The phylogenetic analysis based on both markers ( rbcL and matK ) showed that both A. coriacea and A. tortilis were closely related with each other and clustered in the same group while other two species A. albida and A. catechu were grouped together. The specie A. ampliceps remained ungrouped distantly, compared with other four species. These finding highlights the potential of DNA barcoding for efficient and reproducible identification of Acacia species.

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Section: Introductionmentioning

confidence: 99%

Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers

Ismail

Ahmad

Nadeem

et al. 2020

Saudi Journal of Biological Sciences

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“…Di Indonesia, A. mangium telah menjadi spesies kayu untuk pulp yang mendominasi selama 10 tahun terakhir sebagai sumber serat yang sangat baik untuk pembuatan kertas (Muhammad, Ong and Ratnam, 2018;Hillman, 2002). Hutan Tanaman Industri untuk bahan baku pulp di Indonesia mengembangkan jenis A. mangium Willd dan A. crassicarpa A. Cunn (Suhartati,Rahmayanto and Daeng, 2014).…”

Section: Pendahuluanunclassified

Pengaruh Alkali Aktif terhadap Karakteristik Pulp Kraft Putih Acacia mangium dan Eucalyptus pellita

Kardiansyah

Sugesty

2020

J selulosa

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Industri pulp Indonesia saat ini memiliki masalah dalam penyediaan bahan baku kayu Acacia mangium, karena serangan penyakit tanaman dan hama. Hal ini harus diantisipasi melalui bahan baku alternatif pengganti Acacia mangium, spesies Eucalyptus pellita dipilih karena lebih tahan terhadap hama dan penyakit. Penelitian ini dilakukan untuk mengetahui karakteristik kualitas pulp kraft putih E. Pellita. Penelitian pembuatan pulp kertas dilakukan dengan proses kraft dengan bahan baku A. mangium dan E. pellita. Pemasakan dilakukan dengan variasi alkali aktif 16-20%, sulfiditas 28,7%, pada suhu 165 °C, rasio larutan pemasak terhadap kayu 3,5:1 dan faktor H 1.022. Pemutihan pulp dilakukan dengan proses ECF (Elemental Chlorine Free) dengan tahapan OD0EoD1. Karakteristik pulp kraft hasil pemasakan A. mangium lebih tinggi pada parameter rendemen tersaring, bilangan kappa dan viskositas dibandingkan dengan E. pellita. Penggunaan alkali aktif 16% pada pemasakan A. mangium lebih rendah dari E. pellita (18%), namun kualitasnya dapat memenuhi spesifikasi kualitas pulp kraft putih sesuai SNI 6107:2015 (Pulp Kraft Putih Kayu daun) pada parameter derajat giling, derajat putih dan sifat fisik. Karakteristik pulp kraft putih A. mangium lebih tinggi pada parameter derajat giling, derajat putih dan sifat fisik dibandingkan dengan E. pellita. Namun demikian E. pellita berpotensi untuk dikembangkan di Hutan Tanaman Industri sebagai bahan baku pulp.Effect of Active Alkali on Characteristic of Acacia mangium and Eucalyptus pellita Bleached Kraft PulpAbstractThe Indonesian pulp industry currently has problems in supplying Acacia mangium wood raw materials, due to plant disease and pest attacks. This could be anticipated through alternative raw materials to substitute Acacia mangium, the Eucalyptus pellita species chosen because it is more resistant to pests and diseases. This study was conducted to determine the quality characteristics of E. Pellita bleached kraft pulp. The research on making paper pulp using A. mangium and E. pellita was carried out by means of the kraft process. The cooking was carried out with a variation of 16-20% active alkali, 28.7% sulfidity, at a temperature of 165°C, a liquor to wood ratio of 3.5:1 and an H factor of 1.022. The bleaching of the pulp has been carried out using the ECF (Elemental Chlorine Free) process with the OD0EoD1 stage. Characteristics of kraft pulp from A. mangium cooking were higher in the screening yield, kappa number and viscosity compared to E. Pellita. The use of 16% active alkaline in cooking of A. mangium is lower than E. pellita (18%), but the quality can meet the quality specifications of white kraft pulp according to SNI 6107: 2015 (White Kraft Leaf Wood Pulp) on the parameters of milled degree, whiteness and properties. The characteristics of A. mangium white kraft pulp were higher in parameters of grind degree, whiteness and physical properties compared to E. pellita. However, E. pellita has the potential to be developed in Industrial Plantation Forests as raw material for pulp.

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“…Acacia mangium × A. auriculiformis, a hybrid of A. mangium and A. auriculiformis [1], is a promising tree species with superior characteristics of growth, adaptability, ber quality, pulping properties, and insect and wind resistance compared to the parental species [2][3][4][5][6]. However, asexual propagation is the only way to maintain superiority [7,8].…”

Section: Introductionmentioning

confidence: 99%

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

LieJian

Hong

et al. 2021

Preprint

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Background: Autoclaving is used to eliminate contamination during tissue culturing, however, it is a complicated process, time-consuming and costly. Chemical sterilization of tissue culture can effectively eliminate contamination, is a simple procedure, and cost effective. However, studies on the chemical sterilization mostly focus on bud induction, while the effects of chemical sterilization overall process of tissue culture, including bud induction, proliferation, and rooting, remain to be determined. Here, we investigate the effect of chemical sterilization on bud induction, proliferation, and rooting of Acacia mangium × A. auriculiformis.Results: The results showed that chlorothalonil (0.2 g/L) was a suitable chemical sterilant, and bud induction medium was 1/8 Murashige and Skoog medium + agar 7 g/L + chlorothalonil 0.2 g/L + 6-benzylaminopurine 0.5 mg/L The highest induction rate (99.54%) was observed in the third to fifth buds’ stem segments collected in October treated with 0.8 g/L carbendazim for 3 min, with a contamination rate of 0. The rooting medium was agar 7 g/L + chlorothalonil 0.2 g/L+ indolebutyric acid 1.5 mg/L + naphthylacetic acid 0.5 mg/L, and the rooting rate was 97.62%. The proliferation rate and subculture duration showed a positive correlation, while the proliferation rate was 3.58 times higher at the fourth subculture rooting. Conclusions: Our results suggest that chlorothalonil can effectively replace autoclaving during bud induction, proliferation, and rooting of A. mangium × A. auriculiformis. The findings of this study provide technical support for rapid seedlings propagation, accelerates the breeding process of Acacia, and can be applied in other tree species.

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Characterization of mean stem density, fibre length and lignin from two Acacia species and their hybrid

Cited by 10 publications

References 16 publications

Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers

Development of DNA barcodes for selected Acacia species by using rbcL and matK DNA markers

Pengaruh Alkali Aktif terhadap Karakteristik Pulp Kraft Putih Acacia mangium dan Eucalyptus pellita

Acacia Mangium × A. Auriculiformis Micropropagation in a Non-Sterile Environment

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