Characterization of malignant and non‐neoplastic cell phenotypes in highly malignant non‐Hodgkin lymphomas

Porwit-Ksiazek, A; Christensson, Birger; Lindemalm, C.; Mellstedt, Håkan; Tribukait, Bernhard; Biberfeld, Gunnel; Biberfeld, Peter

doi:10.1002/ijc.2910320604

Cited by 21 publications

(2 citation statements)

References 43 publications

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“…Cytomorphological analyses Cytospins were prepared as described (Porwit‐Ksiazek et al , 1983). Briefly, cells were centrifuged on to slides and fixed in cold acetone or methanol.…”

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Dendritic cells in patients with non‐progressive B‐chronic lymphocytic leukaemia have a normal functional capability but abnormal cytokine pattern

et al. 2001

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Summary. Dendritic cells (DC) are attractive candidates for use in vaccine-based immunotherapy. We have analysed the functional capability of DC generated in vitro from blood CD14 1 cells of chronic lymphocytic leukaemia (CLL) patients and healthy donors by culturing for 10 d with granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 4 (IL-4) and tumour necrosis factor-a (TNF-a). Two distinct DC populations were identified in patients as well as in controls. The majority of DC expressed CD11c and a minority also CD123. Most of the DC generated from both patients and controls exhibited a mature phenotype indicated by CD83 and major histocompatibility complex (MHC) class II expression, as well as by a characteristic morphology. Less than 1% of DC exhibited CD14. CLL DC had a similar expression of accessory molecules (CD54, CD80 and CD86) as control DC. The mean fluorescence intensity of CD80 and MHC class I molecules was significantly higher on CLL DC than on control DC (P , 0´05). At the gene level (real-time polymerase chain reaction) the expression of IL-10 was higher in CLL (P 0´028) than in control DC. IL-1b and IL-12p 35 transcripts were also more abundant in CLL than in control DC but did not reach statistical significance. The expression of IL-4 and TNF-a was similar to that of control DC. The interferon g (IFN-g) gene expression level in CLL DC was decreased compared with control DC. DC of CLL patients had a similar capacity to stimulate in mixed leucocyte reaction as well as to present a recall antigen (PPD) as control DC. Thus, DC of CLL patients seem to have a normal function and may serve as antigen preserving cells for presentation of tumour antigens in a therapeutic vaccination approach. The mechanisms behind the observed increase in some surface molecules and the abnormal cytokine profile of CLL DC is not clear but might indicate pre-activation of DC in vivo, which may have a regulatory role in the pathobiology of CLL.

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“…Cytomorphological analyses Cytospins were prepared as described (Porwit‐Ksiazek et al , 1983). Briefly, cells were centrifuged on to slides and fixed in cold acetone or methanol.…”

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confidence: 99%

Dendritic cells in patients with non‐progressive B‐chronic lymphocytic leukaemia have a normal functional capability but abnormal cytokine pattern

et al. 2001

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“…It has long been recognized that T lymphocytes are consistently present in lymph nodes involved by B-cell non-Hodgkin's lymphomas (B-NHL), in close association with malignant cells (Habeshaw et al, 1983). Earlier studies indicated a favourable prognosis for B-cell lymphoma patients with a high content of tumour-infiltrating lymphocytes-T (TIL-T) and a high percentage of infiltrating CD8+ cytotoxic/suppressor cells (Porwit-Ksiazek et al, 1983;Lippman et aZ, 1990). Recent observations showing the clinical responsiveness of some lymphoma patients to interleukin-2 (IL-2) based immunotherapy suggested that antiturnour immune reactivity may be present in such patients (Weber et af, 1992).…”

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Autotumour reactive T‐cell clones among tumour‐infiltrating T lymphocytes in B‐cell non‐Hodgkin's lymphomas

et al. 1995

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Seventy-three T-cell clones (TCC) were established from tumour-infiltrating lymphocytes-T (TIL-T) derived from lymph nodes involved by B-cell non-Hodgkin's lymphomas (B-NHL) in nine patients with different histological subtypes and clinical stages. 40 TCC (55%) expressed the CD25 Ag and were also able to proliferate in the presence of irradiated autologous B-NHL cells. Among them, 23 autotumour (AuTu) proliferative TCC were found not to proliferate to autologous EBV-transformed B-cell lines, indicating that the proliferative reactivity of these TCC was preferentially directed at autologous B-NHL cells. Tested against autologous B-NHL cells, only three AuTu proliferative TCC (CD8+) showed a significant level of cytotoxicity (specific lysis > 15%). In blocking experiments, the AuTu proliferative reactivity of three TCC from one patient was strongly inhibited by anti-DR and anti-DQ mAbs, whereas that of three TCC from another patient was not affected by either anti-MHC class I or class II (DR, DP, DQ) mAbs. These findings suggest that the recognition of autologous B-NHL cells by AuTu proliferative TCC may occur through MHC-restricted as well as MHC-unrestricted mechanisms.

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Cell proliferation and DNA content in non-hodgkin' s lymphoma: Flow cytometry in relation to lymphoma classification

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et al. 1986

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Flow cytofluorometric (FCF) DNA analysis was performed on specimens from 154 patients with non-Hodgkin's lymphoma (NHL) and correlated to histopathology according to the Rappaport classification and the Working Formulation of NHL for clinical usage (WF). NHL associated with unfavorable prognosis (UP) had significantly higher proportions of cells in S-phase as compared to the favorable prognosis (FP) group. The proportion of S-phase cells also differed significantly between the low-, intermediate-, and high-grade malignancy groups of the WF. Aneuploidy was significantly more frequent in the UP (41%) than in the FP (15%) group. By stepwise discriminant analysis, the S-phase frequency was a stronger discriminator between prognostic groups than DNA content. Classification by discriminant analysis showed that over 90% of the low grade/FP lymphomas were allocated to the good prognosis group by their S-phase and DNA values. Due to the variation in the proportion of S-phase cells in the intermediate grade and high grade, as well as the UP lymphomas, only approximately 50% of the UP and 60% of the high-grade lymphomas were identified as such by this discriminant model, while almost one half of the intermediate-grade lymphomas were allocated to the low-grade group. The results suggest that S-phase analysis can be of value in distinguishing intermediate- and high-grade lymphomas with high and low proportions of proliferating cells, which may be prognostic importance.

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Characterization of malignant and non‐neoplastic cell phenotypes in highly malignant non‐Hodgkin lymphomas

Cited by 21 publications

References 43 publications

Dendritic cells in patients with non‐progressive B‐chronic lymphocytic leukaemia have a normal functional capability but abnormal cytokine pattern

Dendritic cells in patients with non‐progressive B‐chronic lymphocytic leukaemia have a normal functional capability but abnormal cytokine pattern

Autotumour reactive T‐cell clones among tumour‐infiltrating T lymphocytes in B‐cell non‐Hodgkin's lymphomas

Cell proliferation and DNA content in non-hodgkin' s lymphoma: Flow cytometry in relation to lymphoma classification

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