Characterization of lncRNAs involved in drought response in Betula platyphylla

Zhang, Yiming; Li, Li; Wang, Chao; Wang, Yanmin; Zhang, Tengqian; Wang, Yucheng

doi:10.1007/s00468-022-02361-6

Cited by 1 publication

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“…Birch is one of the main broadleaf tree species, with important applications in the pulp and biofuel industries (Borrega et al ., 2013; Li et al ., 2005). To date, many lncRNAs have been identified to be involved in biotic and abiotic stresses (Wen et al ., 2020; Zhang et al ., 2022). In our earlier study, we found that the expression of a birch lncRNA, BplncSIR1 , was induced by salt stress (Jia et al ., 2023).…”

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confidence: 99%

Long noncoding RNA from Betula platyphylla, BplncSIR1, confers salt tolerance by regulating BpNAC2 to mediate reactive oxygen species scavenging and stomatal movement

Jia,

Zhao,

Niu

et al. 2023

Plant Biotechnology Journal

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SummaryLong noncoding RNAs (lncRNAs) play an important role in abiotic stress tolerance. However, their function in conferring abiotic stress tolerance is still unclear. Herein, we characterized the function of a salt‐responsive nuclear lncRNA (BplncSIR1) from Betula platyphylla (birch). Birch plants overexpressing and knocking out for BplncSIR1 were generated. BplncSIR1 was found to improve salt tolerance by inducing antioxidant activity and stomatal closure, and also accelerate plant growth. Chromatin isolation by RNA purification (ChIRP) combined with RNA sequencing indicated that BplncSIR1 binds to the promoter of BpNAC2 (encoding NAC domain‐containing protein 2) to activate its expression. Plants overexpressing and knocking out for BpNAC2 were generated. Consistent with that of BplncSIR1, overexpression of BpNAC2 also accelerated plant growth and conferred salt tolerance. In addition, BpNAC2 binds to different cis‐acting elements, such as G‐box and ‘CCAAT’ sequences, to regulate the genes involved in salt tolerance, resulting in reduced ROS accumulation and decreased water loss rate by stomatal closure. Taken together, BplncSIR1 serves as the regulator of BpNAC2 to induce its expression in response to salt stress, and activated BpNAC2 accelerates plant growth and improves salt tolerance. Therefore, BplncSIR1 might be a candidate gene for molecular breeding to cultivate plants with both a high growth rate and improved salt tolerance.

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