Characterization of lipopeptides from Paenibacillus sp. (IIRAC30) suppressing Rhizoctonia solani

Abstract

The main aim was to identify the active compound against Rhizoctonia solani produced by the cassava endophyte Paenibacillus sp. IIRAC-30. The compounds produced were extracted with ethyl acetate and purified by Sephadex column prior to analysis by Q-TOF mass spectrometry. A C 15 -lipopeptide with an estimated molecular weight of 1036 Da and homologues were identified. The lipopeptide had a cyclic structure, which was deduced by interpreting the ESI-MS/MS spectra of main protonated homologues containing 15:0 FA, and the amino acid composition was Glu-Leu-Leu-Val-Asp-Leu-Leu. Therefore, the lipopeptides produced by isolate IIRAC-30 was characterized as a surfactin series. Thus, the main mechanism used by Paenibacillus sp. IIRAC-30 to suppress R. solani was elucidated. Furthermore, because lipopeptides active against phytopathogens generally show low toxicity to humans and the environment, the positive findings presented here suggest that the isolate IIRAC-30 could be a possible candidate for biocontrol of R. solani.

Novel plant growth promoting rhizobacteria—Prospects and potential

“…Production of antimicrobial compounds, biopreservatives, enzymes and polysaccharides Several crops (Choi et al, 2007;Kurusu et al, 1987;Kaneda, 1996, 1997;Beatty and Jensen, 2002;Karpunina et al, 2003;He et al, 2007;Raza et al, 2010;Canova et al, 2010;Wu et al 2010;Deng et al, 2011) Paenibacillus spp. is also known to elicit the induced systemic resistance (ISR) in plants.…”

Biological control of phytopathogenic fungi by endophytic actinomycetes isolated from maize (Zea mays L.)

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Endophytic microorganisms of tropical tuber crops: Potential and perspectives

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