Characterization of Lancefield group C Streptococcus dysgalactiae isolated from farmed fish

Abstract

A Lancefield group C streptococcal (GCS) infection caused by Streptococcus dysgalactiae that is characterized by severe necrotic lesions of the caudal peduncle has been an increasing cause of mortality in farmed fish such as amberjack, Seriola dumerili, and yellowtail, Seriola quinqueradiata, in the southern part of Kyushu, Japan. In this study, enzymatic profiles of GCS strains from fish and mammals were investigated using the API ZYM system, and genotypic characterization of GCS strains was performed using biased sinusoidal field gel electrophoresis (BSFGE). The partial sequence of the 16S-23S rDNA intergenic spacer region of the GCS strain isolates from fish and mammals was also compared. The API ZYM test indicated that it is difficult to differentiate isolates of S. dysgalactiae from fish and animals based on enzymological variations. In the BSFGE analysis, the macrorestriction profiles, which were obtained using SmaI or ApaI as a restriction enzyme, revealed variations between the fish and animal isolates. The partial sequence of the 16S-23S rDNA intergenic spacer region of all the tested fish isolates differed from all mammalian isolates in one or two nucleotides. The possibility of a clonal expansion of S. dysgalactiae strains in farmed fish was also suggested by the BSFGE profiles of fish isolates.

Isolation and characterization of Streptococcus dysgalactiae from diseased Acipenser schrenckii

“…equisimilis ATCC35666 as well as in accordance with S. dysgalactiae subsp. dysgalactiae ATCC43078 except Naphthol-AS-BI-phosphohydrolase (Nomoto et al, 2006). Their biochemical traits and enzymatic profiles were detailed in Tables 1 and 2 respectively.…”

“…According to enzymatic profiles, these strains were recommended as the subspecies S. dysgalactiae subsp. equisimilis although it needed more proofs (Nomoto et al, 2006;Kawata et al, 2003). Three-fifth and four-fifth healthy sturgeons died after being challenged with the dosage of 4 × 10 8 CFU of sturgeon's isolates and LD50 value of one representative isolate for crucian carps was 2.45 × 10 8 -5.54 × 10 8 CFU even after passage through them three times.…”

“…dysgalactiae ATCC43078 and S. dysgalactiae subsp. equisimilis ATCC35666 were cited from Nomoto et al (2006). molecular analysis.…”

Homogeneity of Streptococcus dysgalactiae from farmed amberjack Seriola dumerili in Japan

“…This division is based on phenotypic and genetic differences in hemolysis, whole-cell protein profiles [6], multilocus enzyme electrophoresis typing, and DNA-DNA relatedness [7]. However, biased sinusoidal field gel electrophoresis (BSFGE) and sodA sequence analysis revealed that S. dysgalactiae isolates obtained from farmed fish differed from those obtained from mammals [8,9].…”

Identification and genotypic analysis of Streptococcus spp. isolated from Giant Pandas in China by PCR-based methods

“…dysgalactiae is an important pathogenic bacterium which is reason of acute or chronic mastitisthe for cattle, goats and sheep (Chenier et al, 2008) even for fish (Nomoto et al, 2006). S. pluranimalium was isolated from panda 3 when it was Alvei profluvium.…”