Characterization of Italian Extra Virgin Olive Oils Using ¹H-NMR Spectroscopy

Abstract

High-field (600-MHz) proton nuclear magnetic resonance (1H-NMR) spectroscopy was applied to the analysis of 55 extra virgin olive oil samples from four Italian regions (Campania, Lazio, Sicily, and Umbria) and obtained from diffrent olive varieties. The multivariate statistical analysis (PCA, hierarchical clustering) performed on the normalized intensities of 1H- NMR resonances due to minor components (β-sitosterol, n-alkenals, trans-2-alkenals, and other volatile compounds) allows a good classification of oil samples obtained from traditional varieties with respect to the region of origin (96% of oils correctly classified). Samples obtained from one new experimental cultivar (FS-17) were not correctly classified, indicating a strong contribution of olive variety on the chemical composition of virgin olive oils. The potential contribution and limits of NMR in the authentication of virgin olive oil geographical origin and variety are discussed. Keywords: Proton NMR spectroscopy; virgin olive oil; hierarchical clustering; geographical origin; olive variety; authentication

Fast screening of turkish olive oil by NMR spectroscopy for geographical determination and discrimination purposes

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“…Among the different leading countries in olive production, olive oils from Spain, Italy and Greece have been analyzed in detail (Sacchi et al, 1998;AlonsoSalces et al, 2010a;Alonso-Salces et al, 2010b;D'Imperio et al, 2007;Sacchi et al, 1996;Hatzakis et al, 2008). However, there are limited reports and insufficient data on the geographical discrimination of olive oils and olive processing from countries such as Jordan and Turkey (Andjelkovic et al, 2009;Degirmencioglu, 2011).…”

Italian and Argentine olive oils: a NMR and gas chromatographic study

“…Fasc. 6 (2001), 380-388 Italian olive oils (Sacchi, 1998, Mannina, 2001a, Mannina, 2001b, Mannina,1999a. 13 C NMR technique provides (Mannina, 1999b(Mannina, , 2000 information about the fatty acid composition and the acyl distribution on the glycerol moiety; this information can be of crucial importance in the analytical characterization of edible oils and in the prevention of frauds such as the addition with esterified oils.…”

Caracterização dos constituintes poliméricos da Maytenus ilicifolia por relaxação nuclear de ¹H por RMN no estado sólido

“…Na literatura, encontram-se vários estudos de caracterização por RMN de produtos naturais. Esta técnica espectroscópica é referida em análises de autenticidade e estudos de avaliação de adulteração em produtos como purês, por exemplo, [15][16][17][18][19][20] . Os resultados apresentados demonstram a potencialidade desta técnica.…”