Fast screening of turkish olive oil by NMR spectroscopy for geographical determination and discrimination purposes

Ok, Salim

doi:10.3989/gya.122413

Cited by 16 publications

(34 citation statements)

References 26 publications

(58 reference statements)

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“…Transversal (T 2 ) relaxation was measured using the standard Carr-Pucell-Meiboom-Gill (CPMG) pulse sequence found in the Bruker library. The T 2 measurements were conducted with a time delay between the 90 o and 180 o pulses (t) of 1.0 ms. Data from 1250 echoes were acquired from 32 scans, and the delay time was chosen long enough, 5*T 1 , to enable complete decay of the T 2 signal (Ok, 2014). The repetition time between two succeeding scans was set to 10 s.…”

Section: Lf 1 H Nmr Relaxometrymentioning

confidence: 99%

Detection of olive oil adulteration by low-field NMR relaxometry and UV-Vis spectroscopy upon mixing olive oil with various edible oils

2017

Grasas y Aceites

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SUMMARY:Adulteration of olive oil using unhealthy substitutes is considered a threat for public health. Low-field (LF) proton (1H) nuclear magnetic resonance (NMR) relaxometry and ultra-violet (UV) visible spectroscopy are used to detect adulteration of olive oil. Three different olive oil with different oleoyl acyl contents were mixed with almond, castor, corn, and sesame oils with three volumetric ratios, respectively. In addition, Arbequina olive oil was mixed with canola, flax, grape seed, peanut, soybean, and sunflower seed oils with three volumetric ratios. Transverse magnetization relaxation time (T 2 ) curves were fitted with bi-exponential decaying functions. T 2 times of each mixture of olive oils and castor oils, and olive oils and corn oils changed systematically as a function of volumetric ratio. To detect the adulteration in the mixtures with almond and sesame oils, both LF 1 H NMR relaxometry and UV-Vis spectroscopy were needed, where UV-Vis-spectroscopy detected the adulteration qualitatively. In the mixtures of Arbequina olive oil and flax, peanut, soybean, and sunflower seed oils, both T 21 and T 22 values became longer systematically as the content of the olive oil was decreased. The unique UV-Vis maximum absorbance of flax oil at 320.0 nm shows the adulteration of olive oil qualitatively. KEYWORDS: Adulteration detection; Low-field NMR relaxometry; Olive oil; UV-Vis spectroscopyRESUMEN: Detección de la adulteración de aceite de oliva mediante relaxometría magnética nuclear de campo bajo y espectroscopía UV-Vis sobre mezcla de aceite de oliva con diversos aceites comestibles. La adulteración del aceite de oliva con sustituyentes menos saludables es una amenaza para la salud pública. En este trabajo, la detección de la adulteración del aceite de oliva se demuestra utilizando tanto relaxometría magnética nuclear de campo bajo (LF) de protones ( 1 H) (RMN) y espectroscopía visible y ultra-violeta (UV). Tres muestras de aceites de oliva con diferentes contenidos en oleico se mezclaron con aceites de almendra, ricino, maíz y sésamo con tres relaciones volumétricas. Además, el de arbequina de California se mezcló con cánola, lino, semilla de uva, cacahuete, soja y aceites de girasol con tres relaciones volumétricas. Las curvas de tiempo de relajación de magnetización transversal (T2) fueron completadas con funciones de decaimiento biexponencial. Los tiempos T2 determinados para cada mezcla de aceite de oliva con ricino y maíz cambian sistemáticamente conforme las relaciones volumétricas. Para detectar la adulteración de los aceites de oliva con aceites de almendra y de sés-amo, se necesitan tanto relaxometría LF 1H RMN como la espectroscopía UV-Vis. En las mezclas de aceite de oliva arbequina con aceites de lino, maní, soja y girasol, ambos valores T21 y T22 se hicieron mayores sistemáti-camente conforme el porcentaje de aceite de oliva se reduce en la mezcla. El máximo de absorbancia UV-Vis del aceite de lino a 320,0 nm demostrará la adulteración del aceite de oliva cualitativamente.

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Section: Lf 1 H Nmr Relaxometrymentioning

confidence: 99%

Detection of olive oil adulteration by low-field NMR relaxometry and UV-Vis spectroscopy upon mixing olive oil with various edible oils

2017

Grasas y Aceites

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“…As preliminary approach to the chemical characterization of the extract, 1 H NMR spectroscopy ( Fig. 1) has been performed evidencing a typical fingerprinting of a fixed oil, with specific chemical shifts of fatty acid esters of glycerol (35,36). Given these 666 A. M. SAAB ET AL.…”

Section: Resultsmentioning

confidence: 99%

Laurus nobilisL. Seed Extract Reveals Collateral Sensitivity in Multidrug-Resistant P-Glycoprotein-Expressing Tumor Cells

Saab

Guerrini

Zeino

et al. 2015

Nutrition and Cancer

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The frequent failure of standard cancer chemotherapy requires the development of novel drugs capable of killing otherwise drug-resistant tumors. Here, we have investigated a chloroform extract of Laurus nobilis seeds. Fatty acids and 23 constituents of the volatile fraction were identified by gas chromotography/flame ionization detection (GC/FID) and gas chromatography/mass spectrometry (GC/MS), in good agreement with (1)H NMR (nuclear magnetic resonance) spectrum. Multidrug-resistant P-glycoprotein-expressing CEM/ADR5000 leukemia cells were hypersensitive (collaterally sensitive) toward this extract compared to drug-sensitive CCRF-CEM cells, whereas CEM/ADR5000 cells were 2586-fold resistant to doxorubicin as control drug. Collateral sensitivity was verified by measurement of apoptotic cells by flow cytometry. The log10IC50 values of 3 compounds in the extract (limonene, eucalyptol, oleic acid) did not correlate with mRNA expression of the P-glycoprotein-coding ABCB1/MDR1 gene and accumulation of the P-glycoprotein substrate rhodamine in the NCI panel of tumor cell lines. A microarray-based profile of 20 genes predicted resistance to doxorubicin and 7 other anticancer drugs involved in the multidrug resistance phenotype but not to limonene, eucalyptol and oleic acid. In conclusion, our results show that Laurus nobilis seed extract is suitable to kill multidrug-resistant P-glycoprotein expressing tumor cells.

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“…The quantitative analysis of the region between 0.00 and 4.00 ppm in 1 H-NMR spectra of olive oil samples was done referring to the literature [6,11]. The resonances at 0.98 ppm (E), 0.88 ppm (F), 2.01 ppm (C), and 2.77 ppm (A) arose from the following functional groups having different number of protons, respectively: -CH 3 (terminal group of Linolenoyl (Ln) acyl ((Ln (18:3)); 3H), -CH 3 (terminal group of Linoleoyl (L) acyl) ((L (18:2)) (3H) + Oleoyl (O) acyl ((O (18:1)) (3H) + saturated fatty acyl (S) (3H); 9H), -CH 2 -CH=CH (allylic group) ((L (18:2)) (4H) + (Ln (18:3)) (4H) + (O (18:1)) (4H); 12H), CH=CH-CH 2 -CH=CH (diallylic group) ((L (18:2)) (2H) + (Ln (18:3)) (4H); 6H).…”

Section: Discussionmentioning

confidence: 99%

“…The integrals of the remaining peaks were determined with respect to the integral value of E. The integral values of the peaks (E, A, C, F) were equalized to the number of corresponding protons. The linear mathematical equations were explained previously [11]. Integration of each peak in the 1 H-NMR spectra was repeated 6 times by using the iNMR 4.3.0 software that reported the frequency range of the integral area.…”

Section: Discussionmentioning

confidence: 99%

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Authentication of Commercial Extra Virgin Olive Oils

2016

J Americ Oil Chem Soc

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The goal of the current contribution is to determine the geographical origins of olive oil samples obtained from eight different countries during the harvest seasons of 2013 and 2014. First, the contents of olive oil samples were quantified by integrating the peaks of 1H‐NMR spectra and using linear mathematical equations. The results were analyzed by analysis of variance (ANOVA), a statistical method examining whether there is a significant difference between the groups mean. The origins of the majority of the olive oil samples were discriminated by ANOVA and the minor constituents of the olive oils. Tocopherol and cycloartenol were the most discriminative minor constituents of the olive oil samples.

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Fast screening of turkish olive oil by NMR spectroscopy for geographical determination and discrimination purposes

Cited by 16 publications

References 26 publications

Detection of olive oil adulteration by low-field NMR relaxometry and UV-Vis spectroscopy upon mixing olive oil with various edible oils

Detection of olive oil adulteration by low-field NMR relaxometry and UV-Vis spectroscopy upon mixing olive oil with various edible oils

Laurus nobilisL. Seed Extract Reveals Collateral Sensitivity in Multidrug-Resistant P-Glycoprotein-Expressing Tumor Cells

Authentication of Commercial Extra Virgin Olive Oils

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