Characterization of Irreversible Electroporation Ablation with a Validated Perfused Organ Model

Effects of internal electrode cooling on irreversible electroporation using a perfused organ model

“…A heat transfer coefficient of 30.01 W=cm 2 Á K was calculated and utilized within the model. The volumetric flow rate was calculated based on the flow rate of the internal cooling (35 ml/ min), and the cross-sectional area of the perfusion inlet stream as shown in Equation (6). Then the Reynolds and Prandtl number were determined and applied to solve for the Nusselt number for turbulent flow within a pipe (Re > 4000)…”

“…Although they are not the main cause of cell death, temperature and current variations during IRE treatment are critical [5]. While studies have shown that higher delivered electrical energy can be associated with larger treatment zones, it also causes an increase in the protein denaturation region due to thermal side effects [6]. This creates challenges in generating a sufficiently high pulsed electric field that concurrently covers the entire region of interest, while avoiding undesirable thermal effects.…”

“…In this study, a comprehensive examination of IRE treatment with an internally perfused applicator is performed. The IRE treatments are performed on a previously validated perfused organ model [6] and the outcomes are investigated in terms of ablation size, temperature, electrical current and generator failure.…”

Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy

“…All IRE treatments were performed in a perfused organ model [13,14]. A total of six livers were used for this study with 6 -8 treatments delivered per liver using a 4-electrode configuration electrode array.…”

“…All experimental IRE procedures were performed on a previously validated perfused organ model [13,14]. Porcine livers were excised at a local abattoir and immediately fit with Luer-Lock connectors at the major ports of the specimen for a constant pressure flush ($90 mmHg) with three liters of modified phosphate buffer solution (conductivity of $0.8 S/ m) to mitigate thrombosis and improve overall tissue perfusion.…”