Characterization of inter-Sertoli cell tight and gap junctions in the testis of turtle: Protect the developing germ cells from an immune response

Ahmed, Nisar; Yang, Ping; Chen, Hong; Ujjan, Imtiaz Ali; Haseeb, Abdul; Wang, Lingling; Soomro, Feroza; Faraz, Shahid; Sahito, Benazir; Ali, Waseem; Chen, Qiusheng

doi:10.1016/j.micpath.2018.06.037

Cited by 23 publications

(13 citation statements)

References 43 publications

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“…In testicular tissue, Sertoli cells are composed of blood-testis-barrier (BTB) with Sertoli cell–cell and Sertoli germ cell junctions, where vital functions in spermatogenesis are effectuated (Ahmed et al. 2018 ; Luca et al. 2018a ).…”

Section: Introductionmentioning

confidence: 99%

SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

Zhou

Wang

Zhao

et al. 2021

Pharmaceutical Biology

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Context Lycium barbarum L. (Solanaceae) seed oil (LBSO) exerts LBSO exerts protective effects in the testis in vivo and in vitro via upregulating SIRT3. Objective This study evaluates the effects and mechanism of LBSO in the d -galactose ( d -gal)-induced ageing testis. Materials and methods Male Sprague Dawley (SD) rats ( n = 30, 8-week-old) were randomly divided into three groups: LBSO group ( n = 10) where rats received subcutaneous injection of d -gal at 125 mg/kg/day for 8 weeks and intragastric administration of LBSO at 1000 mg/kg/day for 4 weeks, ageing model group ( n = 10) received 8-week-sunbcutaneous injection of d -gal, and control group ( n = 10) with same administration of normal saline. Lentivirus had established TM4 cells with SIRT3 overexpression or silencing before LBSO intervened in vitro . Results Treatment with LBSO, the levels of INHB and testosterone both increased, compared to ageing model. In vitro , we found the ED 50 of LBSO was 86.72 ± 1.49 and when the concentration of LBSO at 100 μg/mL to intervene TM4 cells, the number of cells increased from 8120 ± 676.2 to 15251 ± 1119, and the expression of SIRT3, HO-1, and SOD upregulated. However, HO-1 and SOD were dysregulated by silencing SIRT3. On the other hand, the expression of AMPK and PGC-1 α upregulated as an effect of SIRT3 overexpression by lentivirus, meanwhile the same increasing trend of that being found in cells treated with LBSO, compared to control group. Discussion and conclusions LBSO alleviated oxidative stress in d -gal-induced sub-acutely ageing testis and TM4 cells by suppressing the oxidative stress to mitochondria via SIRT3/AMPK/PGC-1α.

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Section: Introductionmentioning

confidence: 99%

SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

Zhou

Wang

Zhao

et al. 2021

Pharmaceutical Biology

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“…8 ), which will bind to actin via the zonula occludens-1, -2 and -3 (ZO-1, ZO-2 and ZO-3) in SCs 9 . At stage VII-VIII of the epithelial cycle, the preleptotene and leptotene spermatocytes must move through the BTB and enter the adluminal compartment 10 . Most researchers focused on the synchronization of spermatogenesis currently, but few of them have addressed the issue from the perspective of Sertoli cells [11][12][13][14][15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

2019

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The functions of Sertoli cells in spermatogenesis have attracted much more attention recently. Normal spermatogenesis depends on Sertoli cells, mainly due to their influence on nutrient supply, maintenance of cell junctions, and support for germ cells’ mitosis and meiosis. Accumulating evidence in the past decade has highlighted the dominant functions of the MAPK, AMPK, and TGF-β/Smad signaling pathways during spermatogenesis. Among these pathways, the MAPK signaling pathway regulates dynamics of tight junctions and adherens junctions, proliferation and meiosis of germ cells, proliferation and lactate production of Sertoli cells; the AMPK and the TGF-β/Smad signaling pathways both affect dynamics of tight junctions and adherens junctions, as well as the proliferation of Sertoli cells. The AMPK signaling pathway also regulates lactate supply. These signaling pathways combine to form a complex regulatory network for spermatogenesis. In testicular tumors or infertile patients, the activities of these signaling pathways in Sertoli cells are abnormal. Clarifying the mechanisms of signaling pathways in Sertoli cells on spermatogenesis provides new insights into the physiological functions of Sertoli cells in male reproduction, and also serves as a pre-requisite to identify potential therapeutic targets in abnormal spermatogenesis including testicular tumor and male infertility.

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“…The junctions between adjacent Sertoli cells also comprise the basal ES at the BTB, along with desmosomes, gap junctions, and tight junctions (30). Spermatocytes translocate to the adluminal compartment via the BTB at specific seminiferous epithelial cycle stages (36). Considering that Sertoli cells are the main component in the BTB and ES, we assume that GGCX and its substrates in Sertoli cells exert particular functions in spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Vitamin K-Dependentγ-Glutamyl Carboxylase in Sertoli Cells Is Essential for Male Fertility in Mice

Shiba

Ikeda

Horie‐Inoue

et al. 2021

Molecular and Cellular Biology

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Gamma-glutamyl carboxylase (GGCX) is a vitamin K (VK)-dependent enzyme that catalyzes γ-carboxylation of glutamic acid residues in VK-dependent proteins. Anticoagulant warfarin is known to reduce GGCX activity by inhibiting VK cycle and is recently shown to disrupt spermatogenesis. To explore GGCX function in testis, we here generated Sertoli cell-specific Ggcx conditional knockout (Ggcx scKO) mice and investigated their testicular phenotype. Ggcx scKO mice exhibited late-onset male infertility. They possessed morphologically abnormal seminiferous tubules containing multinucleated and apoptotic germ cells, and their sperm concentration and motility were substantially reduced. Localization of connexin 43 (Cx43), a gap junction protein abundantly expressed in Sertoli cells and required for spermatogenesis, was distorted in Ggcx scKO testes, and Cx43 overexpression in Sertoli cells rescued the infertility of Ggcx scKO mice. These results highlight GGCX activity within Sertoli cells, which promotes spermatogenesis by regulating the intercellular connection between Sertoli cells and germ cells.

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Characterization of inter-Sertoli cell tight and gap junctions in the testis of turtle: Protect the developing germ cells from an immune response

Cited by 23 publications

References 43 publications

SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis

Vitamin K-Dependentγ-Glutamyl Carboxylase in Sertoli Cells Is Essential for Male Fertility in Mice

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