SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses

Abstract

Context Lycium barbarum L. (Solanaceae) seed oil (LBSO) exerts LBSO exerts protective effects in the testis in vivo and in vitro via upregulating SIRT3. Objective This study evaluates the effects and mechanism of LBSO in the d -galactose ( d -gal)-induced ageing testis. Materials and methods Male Sprague Dawley (SD) rats ( n = 30, 8-week-old) were randomly divided into three groups: LBSO group ( n = 10) where rats received subcutaneous injection of d -gal at 125 mg/kg/day for 8 weeks and intragastric administration of LBSO at 1000 mg/kg/day for 4 weeks, ageing model group ( n = 10) received 8-week-sunbcutaneous injection of d -gal, and control group ( n = 10) with same administration of normal saline. Lentivirus had established TM4 cells with SIRT3 overexpression or silencing before LBSO intervened in vitro . Results Treatment with LBSO, the levels of INHB and testosterone both increased, compared to ageing model. In vitro , we found the ED 50 of LBSO was 86.72 ± 1.49 and when the concentration of LBSO at 100 μg/mL to intervene TM4 cells, the number of cells increased from 8120 ± 676.2 to 15251 ± 1119, and the expression of SIRT3, HO-1, and SOD upregulated. However, HO-1 and SOD were dysregulated by silencing SIRT3. On the other hand, the expression of AMPK and PGC-1 α upregulated as an effect of SIRT3 overexpression by lentivirus, meanwhile the same increasing trend of that being found in cells treated with LBSO, compared to control group. Discussion and conclusions LBSO alleviated oxidative stress in d -gal-induced sub-acutely ageing testis and TM4 cells by suppressing the oxidative stress to mitochondria via SIRT3/AMPK/PGC-1α.

Melatonin ameliorates PM2.5‐induced spermatogenesis disorder by preserving H3K9 methylation and SIRT3

“…Furthermore, expression of was accelerated by melatonin therapy in reactive to PM2.5 exposure, but SIRT1 was not. The obtained results were consistent with those studied by Yang et al, 39 who reported that melatonin could increase the SIRT3 expression in testicular cells, manifesting a probable functional interaction between SIRT3 and melatonin in PM2.5-caused apoptosis. Then, to ascertain whether SIRT3 played a crucial part in mediating the observed protective action of melatonin after PM2.5 exposure, GC-2 cells were pretreated with 3-TYP, the specifical inhibitor of SIRT3.…”

Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling Pathway

“…Moreover, following the treatment with the inhibitors of AMPK (compound C) and SIRT3 (3-TYP), there was an antagonism effect on Curcumin, and the TJ proteins (namely, ZO-1, Occludin, and Claudin-11) in D-gal-treated SCs presented lowered expressions. Intriguingly, SIRT3 was discovered to activate AMPK in several disease models, according to previous studies [45][46][47]. Moreover, it has been reported that Lycium barbarum seed oil stimulates testosterone secretion and reduces oxidative stress partly via the activation of the SIRT3/AMPK signaling pathway in subacutely aging murine testes, and SIRT3 silently decreases the AMPK phosphorylation in TM4 cells [47].…”

“…Intriguingly, SIRT3 was discovered to activate AMPK in several disease models, according to previous studies [45][46][47]. Moreover, it has been reported that Lycium barbarum seed oil stimulates testosterone secretion and reduces oxidative stress partly via the activation of the SIRT3/AMPK signaling pathway in subacutely aging murine testes, and SIRT3 silently decreases the AMPK phosphorylation in TM4 cells [47]. Nonetheless, there is growing evidence that AMPK has a regulatory effect on SIRT3.…”

The Medicinal Species of the Lycium Genus (Goji Berries) in East Asia: A Review of Its Effect on Cell Signal Transduction Pathways

“…Antioxidant effect in mitochondria, vascular protection [6,30] 3.1. NF-κB Signal Transduction Pathway NF-κB is a class of nuclear transcription factors that play a significant role in regulating the expression of genes.…”