Characterization of insulin-loaded liposome using column-switching HPLC

Ohnishi, Naozumi; Tanaka, Shota; Tahara, Kohei; Takeuchi, Hirofumi

doi:10.1016/j.ijpharm.2014.12.056

Cited by 17 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ultimately, DiI-LipoXL-01 was composed of 0.75 mg of DiI, 1.8 mg of XL-01, 10 mg of DPPC, 1.33 mg of Chol, and 2.44 mg of PEG 2000 -DSPE, which was characterized as the size of 132.3 nm, PDI of 0.18, and ζ-potential values of −34.6 mV. We chose an ultracentrifugation method − to determine the EE of DiI in DiI-LipoXL-01 the same as that of XL-01 in LipoXL-01. The EE of DiI was 95.2%.…”

Section: Resultsmentioning

confidence: 99%

Novel 5-Fluorouracil Carbonate-Loaded Liposome: Preparation, In Vitro, and In Vivo Evaluation as an Antitumor Agent

Wang

Feng

et al. 2022

Mol. Pharmaceutics

View full text Add to dashboard Cite

5-Fluorouracil (5-FU) is a chemotherapeutic drug against many types of cancers, especially colorectal cancer. However, its short plasma half-life and serious adverse reactions limit its wide clinical applications. To overcome these shortcomings, a novel lipophilic 5-FU carbonate [XL-01, (5-fluoro-2,4dioxo-3,4-dihydropyrimidin-1(2H)-yl) methyl tetradecyl carbonate] was designed, synthesized, and encapsulated into liposome (LipoXL-01) by a thin-film dispersion method through formulation screening and optimization. LipoXL-01 was characterized by a particle size of around 100 nm, polydispersity index of 0.200, ζpotential value of −41 mV, encapsulation efficiency of 93.9%, and drug-loading efficiency of 11.6%. The cellular uptake of LipoXL-01 was increased in a concentration-dependent manner on HCT15 cells. LipoXL-01 could enhance the induction of cell apoptosis and the inhibition of cell migration and arrest the ability of the cell cycle at the S-phase on HCT15 cells better than 5-FU. Additionally, LipoXL-01 exhibited a slow drug release profile with a cumulative release rate of 12% in 8 h. The results of pharmacokinetic and biodistribution studies revealed that LipoXL-01 had a long plasma half-life (7.21 h) and a high tumor accumulation (733 nmol/g at 8 h). The in vivo antitumor effect study also showed that LipoXL-01 had more potent efficacy than 5-FU (65 vs 48% of the tumorinhibition rate). Simultaneously, negligible systemic toxicity was observed via analyzing the body weight as well as hematological and pathological parameters in the tested mice. The current study suggested that LipoXL-01 might be a promising nanocandidate for chemotherapy of colorectal cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Novel 5-Fluorouracil Carbonate-Loaded Liposome: Preparation, In Vitro, and In Vivo Evaluation as an Antitumor Agent

Wang

Feng

et al. 2022

Mol. Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…Active targeting, which tends to be more efficient, is achieved by the tailored design of nanomedicines through conjugating ligand on its surface to receptors on specific cell membranes of the tumour ( Alabsi et al, Aug. 2021 ). Therapeutics including small molecules, peptides, proteins, and nuclei acids, have been studied to be engineered into inhalable nanomedicines for disease treatment ( Tran et al, 2020 )– ( Ohnishi et al, Feb. 2015 ). Apart from treating respiratory and systemic diseases, inhaled nanomedicines also presents potential applications in biomedical diagnostics and imaging ( Gaspar et al, Sep. 2008 ).…”

Section: Advancements On Dpi Formulationsmentioning

confidence: 99%

The future of dry powder inhaled therapy: Promising or discouraging for systemic disorders?

Zhu

2022

International Journal of Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…The percent encapsulation of insulin was quite low for 1 mg and increased with increasing amounts of insulin. This system requires quite large amounts of insulin for targeted drug delivery [51,58,61]. .…”

Section: Encapsulation Efficiencymentioning

confidence: 99%

“…Liposomes being versatile amphiphilic carriers have long been used in this regard. It has been used in oral drug delivery [44][45][46], transdermal [47][48][49][50], intravenous delivery [50] and in many other applications [51][52][53].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Phospholipid Nanoparticle Formulations Using Response Surface Methodology

Naeem

Kiew

Chung

et al. 2015

J Surfact & Detergents

View full text Add to dashboard Cite

The present study deals with the optimization of phospholipid liposome formulations to mimic red blood cells. Optimization of different concentrations of distearylphosphatidylcholine, dipalmitoylphosphatidylcholine, and phosphatidylserine at a fixed concentration of lecithin and Tween® 80 was done using response surface methodology. The optimized formulation produced liposomes with a particle size in the range of 112–196 nm. The optimized formulation shows low encapsulation efficiency at low levels of insulin but increases at higher loading levels. Formulated vesicles fulfill the size requirement for intravenous drug delivery. The present system is environmentally friendly with respect to biodegradability and biocompatibility.

show abstract

Characterization of insulin-loaded liposome using column-switching HPLC

Cited by 17 publications

References 19 publications

Novel 5-Fluorouracil Carbonate-Loaded Liposome: Preparation, In Vitro, and In Vivo Evaluation as an Antitumor Agent

Novel 5-Fluorouracil Carbonate-Loaded Liposome: Preparation, In Vitro, and In Vivo Evaluation as an Antitumor Agent

The future of dry powder inhaled therapy: Promising or discouraging for systemic disorders?

Optimization of Phospholipid Nanoparticle Formulations Using Response Surface Methodology

Contact Info

Product

Resources

About