Characterization of indigenous rhizobia from wild legumes

Ahmad, Mohammad; Uddin, M. R.; McLaughlin, Wayne

doi:10.1111/j.1574-6968.1984.tb01304.x

Cited by 27 publications

(9 citation statements)

References 19 publications

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“…The isolates were characterized by colony morphology and acid/alkaline production on YEMA plus 25μgml -1 Bromothymoleblue (BTB) media (Ahmed et al, 1984). Growth rate of the rhizobial isolates was estimated by measuring optical density (OD) using spectrometer from YEM-broth.…”

Section: Morphological Growth and Biochemical Characteristics Of The Ismentioning

confidence: 99%

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

Kenasa¹,

Jida²,

Assefa³

2014

Sci. Technol. Arts Res. J.

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Article InformationSome species of rhizobial bacteria nodulating faba bean are characterized by phosphate solubilization. In order to study their in vitro and symbiotic characteristics, twelve rhizobial isolates nodulating faba bean were collected from acidic soil of Wollega, Ethiopia. Solubilization index of the isolates ranges from 1.25 to 2.10. Mean Generation Time of the isolates were less than 2.34; and growth of isolates on Yeast Extract Mannitol agar with bromothymoleblue media were accompanied by change of color from blue to yellow. AUAVR-51 and AUAVR-52 were the highest in TCP-Solubilizion Index. The two isolates were also recognized as stress tolerant when tested in vitro for extreme temperature, osmotic, acidic pH, intrinsic antibiotics, and acidic-aluminum as compared to the other isolates. However, there was no unique metabolic diversity and specialization of AUAVR-51 and AUAVR-52 isolates with respect to carbon and nitrogen source utilization. On the other hand, isolates AUAVR-51 and AUAVR-52 were characterized by effective and highly effective symbiosis on sterile potted sand growth, respectively. In general, phosphate solubilizing rhizobia nodulating faba bean from acidic soil are fast grower; and their solubilization potential varies. However, the contribution of these isolates as double fertilizer should be tested in the real acidic soil characterized by immobilized phosphorous.

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Section: Morphological Growth and Biochemical Characteristics Of The Ismentioning

confidence: 99%

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

Kenasa¹,

Jida²,

Assefa³

2014

Sci. Technol. Arts Res. J.

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“…Colony diameter and morphology were recorded as SD (small dry), LM (large mucoid) or LW (large watery) according to Ahmed et al (1984). Acid or alkaline production of the strains was detected on YMA containing 0.5% Bromothymol blue (YMA-BTB).…”

Section: Cultural Characteristicsmentioning

confidence: 99%

The Effect of Indigenous Root-Nodulating Bacteria on Nodulation and Growth of Faba Bean (Vicia Faba) in the Low-Input Agricultural Systems of Tigray Highlands, Northern Ethiopia

Workalemahu¹

2009

mejs

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This study was initiated to isolate and characterize indigenous rhizobia nodulating faba bean, and evaluate symbiotic characteristics between the crop and the rhizobia in major faba bean producing areas in Tigray highlands. Field crops were also surveyed for nodulation in selected sites of seven (7) faba bean growing districts. A total of 44 rhizobial strains were isolated and their symbiotic properties (nodule number, fresh weight, color and plant growth) were recorded. A total of 12 strains with good symbiotic properties were characterized for their morphological and physiological traits on Yeast Extract Manitol Agar (YMA) medium. The result of the study showed the presence of diversity in morphological, physiological and symbiotic properties among the rhizobial strains. There was statistically significant difference in nodule number, fresh weight and shoot height (p< 0.05) and great variation in nodule color within and among the districts. Nodulation status of the field crops was poor except Ofla, D/Temben and Alaje districts. Though the presence of diversity among the strains revealed the possibility of getting potentially effective adaptable rhizobial strains that enhance faba bean productivity, the weak symbiotic properties observed during isolation and nodulation status survey might partly be responsible for yield variation and reduction in low input cropping systems of Tigray. Hence, studies on a need for inoculation and factors responsible for poor nodulation need to be undertaken to realize the role of biological nitrogen fixation in Tigray cropping systems.

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“…IRC256 [6]. From our data we can suggest that Nod mutant (JRW3-Sm o) is more competitive in occupying the nodulation sites than Nod + strain.…”

Section: Unguiculata )mentioning

confidence: 60%

“…strains JRW3 [6], IRC256 [7], the medium and the growth conditions for culturing rhizobia have been described previously [8]. strains JRW3 [6], IRC256 [7], the medium and the growth conditions for culturing rhizobia have been described previously [8].…”

Section: Rhizobia Media and Growth Conditionsmentioning

confidence: 99%

Competitive interaction between non-nodulating and nodulating strains for nodulation of cowpea (Vigna unguiculata)

Singh¹

1991

FEMS Microbiology Letters

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We have examined the effect of a non‐nodulating mutant (JRW3‐SmD) on the nodulation ability of cowpea rhizobia (Bradyrhizobium sp.) strains JRW3 and IRC256. Nodulation of cowpea (Vigna unguiculata) by a nodulating Rhizobium strain is suppressed by the presence of a non‐nodulating mutant. The msgnitude of suppression for nodule formation by nodulating strains varied between 40% and 80% depending on the strain and the time of inoculation.

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Characterization of indigenous rhizobia from wild legumes

Cited by 27 publications

References 19 publications

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

The Effect of Indigenous Root-Nodulating Bacteria on Nodulation and Growth of Faba Bean (Vicia Faba) in the Low-Input Agricultural Systems of Tigray Highlands, Northern Ethiopia

Competitive interaction between non-nodulating and nodulating strains for nodulation of cowpea (Vigna unguiculata)

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