The Effect of Indigenous Root-Nodulating Bacteria on Nodulation and Growth of Faba Bean (Vicia Faba) in the Low-Input Agricultural Systems of Tigray Highlands, Northern Ethiopia

Abstract

This study was initiated to isolate and characterize indigenous rhizobia nodulating faba bean, and evaluate symbiotic characteristics between the crop and the rhizobia in major faba bean producing areas in Tigray highlands. Field crops were also surveyed for nodulation in selected sites of seven (7) faba bean growing districts. A total of 44 rhizobial strains were isolated and their symbiotic properties (nodule number, fresh weight, color and plant growth) were recorded. A total of 12 strains with good symbiotic properties were characterized for their morphological and physiological traits on Yeast Extract Manitol Agar (YMA) medium. The result of the study showed the presence of diversity in morphological, physiological and symbiotic properties among the rhizobial strains. There was statistically significant difference in nodule number, fresh weight and shoot height (p< 0.05) and great variation in nodule color within and among the districts. Nodulation status of the field crops was poor except Ofla, D/Temben and Alaje districts. Though the presence of diversity among the strains revealed the possibility of getting potentially effective adaptable rhizobial strains that enhance faba bean productivity, the weak symbiotic properties observed during isolation and nodulation status survey might partly be responsible for yield variation and reduction in low input cropping systems of Tigray. Hence, studies on a need for inoculation and factors responsible for poor nodulation need to be undertaken to realize the role of biological nitrogen fixation in Tigray cropping systems.

Crecimiento De Haba en Simbiosis Con Microorganismos Nativos De Regiones Productoras Del Norte De Puebla, México

“…La concentración en el inóculo se ajustó a 10 9 células mL -1 (Somasegaran y Hoben, 1994). Para la inoculación se aplicó 1 mL del cultivo bacteriano a cada maceta con los cultivos de haba en una sola ocasión y a los 30 d después de la germinación, según lo propuesto por Workalemahu (2009).…”

“…Algunos trabajos sugieren que la fertilización fosfórica incrementa el rendimiento de grano de haba y en manuales agrícolas se sugiere una fertilización química de 200 kg ha -1 de 16N-46P-00K (Morales et al, 2002;Peralta et al, 2013). No obstante, se considera importante encontrar alternativas para cambiar el uso de agroquímicos por biofertilizantes a base de hongos y bacterias simbiontes, ya que la asociación de estos microorganismos no solamente incrementa la producción de flores y frutos, también mejora la calidad de los suelos (Gianinazzi et al, 2010;Smith y Read, 2008;Workalemahu, 2009).…”

Response of Faba Bean to Starter Nitrogen Dose Application and Rhizobial Inoculation in the Major Growing Areas of Arsi Zone

“…Currently rhizobial inoculants are widely used in various parts of the world. They are the solution to dwindling soil fertility, inexpensive, environment friendly, and easy to use with no side effects in most cases (Wondwosen Tena et al, 2016).In response to this, promising Faba Bean Rhizobia screening activities were carried out during the past ten years in the country and revealed that there is diversity in different agroecologies (Abere Mnalku et al, 2009;Alemayehu Workalemahu, 2009;Zerihun Belay and Fassil Assefa, 2011;Anteneh Argaw, 2012;Solomon Legesse and Fassil Assefa, 2014;Dereje Tsegaye et al, 2015;Getahun Negash 2015;Wendesen Melak et al, 2018). Indeed,he symbiotic interaction is initiated after an initial exchange of signals: when nitrogen present in the soil is scarce, legumes exude a series of phenolic compounds into the rhizosphere, mainly flavonoids and isoflavonoid (Clúa et al, 2018),however, Crop yield increases of 51-158% were reported in nitisols at Holleta due to the combined application of 20 kg ha-1P with strain over non-inoculated ones ,none of the above studies reported on the agronomic efficiency, profitability and the level of risk associated with applying starter N fertilizer on Faba bean.…”

Isolation and Biochemical Characterization of Bacteria Isolated from Groundnut (Arachis hypogaea L.) in Sudan

“…The positive correlation observed between nodulation and shoot dry weight of groundnut suggests that the two parameters evolve together (Ngo Nkot et al, 2011). Alemayehu (2010) reported a positive correlation between nodulation and shoot height of V. faba. Inoculation with different strains of Bradyrhizobium significantly improved shoot fresh and dry weight, number of pods and nodules, seed quality and yield.…”