Characterization of in Vitro Metabolism of Loganin by Human Intestinal Microflora Using Ultra-High Performance Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry

Zhao, Min; Xu, Jun; Qian, Dawei; Guo, Jianming; Shang, Erxin; Duan, Jin‐Ao; Du, Lijun

doi:10.1080/00032719.2013.876543

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…Meanwhile, it was reported that the metabolites of loganin were detected in bile, rat urine and feces after oral administration, indicating loganin might influence the intestinal function . Zhao et al also demonstrated the metabolites of loganin could be found in human intestinal bacteria . However, the role of loganin in development of IBD has not been elucidated yet.…”

Section: Introductionmentioning

confidence: 99%

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Loganin alleviates LPS‐activated intestinal epithelial inflammation by regulating TLR4/NF‐κB and JAK/STAT3 signaling pathways

Wang

Pan

Cao

et al. 2019

The Kaohsiung J of Med Scie

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Inflammatory bowel disease (IBD) is a chronic, recurrent gastrointestinal inflammation that affects millions of people around the world. Loganin, an iridoid glycoside, has shown the anti‐inflammatory effects. However, the effect of loganin on IBD and its underlying molecular mechanism are not clear. The present study aimed to investigate whether loganin could alleviate IBD and its mechanisms. The intestinal epithelial Caco‐2 cell line was treated with lipopolysaccharide (LPS) to establish an in vitro IBD model. MTT assay was used to detect cell viability. The expression and release level of inflammatory factors were determined by both real‐time‐PCR and ELISA. Western blotting was used to assess the NF‐κB and JAK/STAT3 pathway‐related protein levels. The results showed that loganin repressed the expression and release of IL‐6, TNF‐α, and IL‐1β, and inhibited TLR4/NF‐κB and JAK/STAT3 signaling pathways in a concentration‐dependent manner. Overexpression of TLR4 could reverse the effect of loganin, leading to activation of NF‐κB signaling and production of inflammatory factors. Meanwhile, IGF‐1, a JAK/STAT3 signaling activator, could also reverse the anti‐inflammation effect of loganin. In conclusion, loganin inhibited LPS‐activated intestinal epithelial inflammation by repressing TLR4/NF‐κB and JAK/STAT3 signaling pathway.

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Section: Introductionmentioning

confidence: 99%

“…9 Zhao et al also demonstrated the metabolites of loganin could be found in human intestinal bacteria. 10 However, the role of loganin in development of IBD has not been elucidated yet.…”

Section: Introductionmentioning

confidence: 99%

Loganin alleviates LPS‐activated intestinal epithelial inflammation by regulating TLR4/NF‐κB and JAK/STAT3 signaling pathways

Wang

Pan

Cao

et al. 2019

The Kaohsiung J of Med Scie

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“…Among them, five metabolites (M1–M4, M10) were obtained from the rat plasma, two metabolites (M4, M9) were from the rat urine and three metabolites (M3, M8, M9) were acquired in the fecal samples (Zhao et al, 2017). In another study by Zhao et al, loganin was metabolized to loganetin (M5), hydrogenated and hydroxylated loganin sulfate (M11), acetylated loganin (M12), hydrogenated and hydroxylated loganetin (M13), and methylated loganetin (M14) (Zhao et al, 2014). By using microbial transformation methods, two metabolites were isolated and identified, one of which was the loganetin obtained after the deglycation of loganin.…”

Section: Pharmacokinetics Of Loganinmentioning

confidence: 99%

Phytochemistry, synthesis, analytical methods, pharmacological activity, and pharmacokinetics of loganin: A comprehensive review

Zhang

et al. 2022

Phytotherapy Research

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Iridoid glycosides (IGs) are found in many medicinal and edible plants, such as Gardenia jasminoides, Cistanche tubulosa, Eucommia ulmoides, Rehmanniae Radix, Lonicera japonica, and Cornus officinalis. Loganin, an IG, is one of the main active ingredient of Cornus officinalis Sieb. et Zucc., which approved as a medicinal and edible plant in China. Loganin has been widely concerned due to its extensive pharmacological effects, including anti‐diabetic, antiinflammatory, neuroprotective, and anti‐tumor activities, etc. Studies have shown that these underlying mechanisms include anti‐oxidation, antiinflammation and anti‐apoptosis by regulating a variety of signaling pathways, such as STAT3/NF‐κB, JAK/STAT3, TLR4/NF‐κB, PI3K/Akt, MCP‐1/CCR2, and RAGE/Nox4/p65 NF‐κB signaling pathways. In order to better understand the research status of loganin and promote its application in human health, this paper systematically summarized the phytochemistry, analysis methods, synthesis, pharmacological properties and related mechanisms, and pharmacokinetics based on the research in the past decades.

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“…Seven prototypes and three metabolites that belonged to iridoid glycosides were previously identified in microdialysis samples from rats after intragastric administration of FC [13]. There were also researches focused on the in vitro metabolism of iridoids such as loganin, sweroside, or morroniside by intestinal microflora by means of UPLC with quadrupole TOF-MS (UPLC-Q-TOF-MS) applications [14][15][16]. Moreover, in vitro metabolites and related transformation pathways of loganin and morroniside by gut microflora were summarized through retention time behavior and mass fragment data [17].…”

Section: Introductionmentioning

confidence: 99%

Investigate the metabolic profile and potential active compounds of Fructus Corni in the treatment of heart failure by applying ultra‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry and network pharmacology

Wang

Chen

et al. 2022

J of Separation Science

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Fructus Corni has been reported to contain a wide variety of pharmacological effects and previous studies had revealed that Fructus Corni might protect the cardiac indices. However, the all-encompassing metabolic profile of Fructus Corni has not been well illuminated. In this research, high-sensitivity ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry method was adopted to identify the metabolic profile after oral administration of Fructus Corni extract, especially the metabolic characterization of serum and heart, for which the targets and signaling pathways about heart failure were hunted through compound-target-disease-pathway intersection network. Ultimately, 37 ingredients were identified in Fructus Corni extract, and 22 prototypes and 134 metabolites that were identified in serum, heart, feces, and urine were tentatively characterized, which contained iridoids, flavonoids, tannins, organic acids, and others. Additionally, 10 putative key compounds including four prototypes and six phase I metabolites were screened by network pharmacology and molecular docking, among which, secoxyloganin (P7), loganin (P14), cornuside III (P17) and cornuside (P20) were the absorbed compounds to represent the potential active ingredients of Fructus Corni engaged in heart failure condition. In general, this method provided the combined strategy to preliminarily settle the complex of Fructus Corni's metabolic profiling and anti-heart failure pharmacologic activities.

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Characterization of in Vitro Metabolism of Loganin by Human Intestinal Microflora Using Ultra-High Performance Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry

Cited by 5 publications

References 38 publications

Loganin alleviates LPS‐activated intestinal epithelial inflammation by regulating TLR4/NF‐κB and JAK/STAT3 signaling pathways

Loganin alleviates LPS‐activated intestinal epithelial inflammation by regulating TLR4/NF‐κB and JAK/STAT3 signaling pathways

Phytochemistry, synthesis, analytical methods, pharmacological activity, and pharmacokinetics of loganin: A comprehensive review

Investigate the metabolic profile and potential active compounds of Fructus Corni in the treatment of heart failure by applying ultra‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry and network pharmacology

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