Characterization of immunogenic proteins of Trypanosoma evansi isolated from three different Indian hosts using hyperimmune sera and immune sera

Laha, R.; Sasmal, N. K.

doi:10.1016/j.rvsc.2008.02.011

Cited by 10 publications

(11 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 62-66 kDa polypeptide cluster was found to be recognized by the immune serum of all the infected animals including cattle, buffalo, and equines. Immunodominant bands in a similar molecular weight range have been reported previously in different studies (Giardina et al, 2003;Laha and Sasmal, 2008;Aquino et al, 2010;Yadav et al, 2013).…”

Section: Discussionsupporting

confidence: 84%

Sero-prevalence and immunological characterization of Trypanosoma evansi infection in livestock of four agro-climatic Zones of Himachal Pradesh, India

Sharma

Gupta

Sethi

et al. 2021

Preprint

View full text Add to dashboard Cite

Trypanosoma evansi, a hemoflagellate protozoan parasite causes wasting disease called surra in wide range of animals. Although, the organism has been reported from various parts of the India, data generated from organized epidemiological study is still in infancy in majority states of India. In the present study, livestock of Himachal Pradesh, India was targeted for epidemiological investigation of T. evansi infections. A total of 440 equines and 444 cattle serum samples were collected from four agro-climatic Zones. Further, serum samples of 280 buffaloes from three different agro-climatic Zones of Himachal Pradesh were also collected and evaluated for presence of T. evansi infection by indirect ELISA. Data generated showed higher prevalence in buffalo (23.57%) followed by cattle (22.52%) and equines (1.82%). Disease was found to be more prevalent (P < 0.05) in cattle of lower altitude as compared to those of higher altitudes. No significant variation was seen in prevalence of disease on the basis of age and sex of the animals. Serum biochemical analysis revealed increased levels of BUN in T. evansi infected equines. Levels of liver function enzymes such as ALT/GGT and AST were found to be significantly elevated (P < 0.01) in infected animals whereas glucose levels were significantly lower in surra infected animals as compared to non-infected animals. Western blot analysis of whole cell lysate (WCL) antigen of T. evansi using surra infected serum samples of equines showed immunodominant bands in the range of 100-25 kDa. Surra infected bovine serum samples recognized polypeptide bands in the range of 85-32 kDa, including protein clusters of 52-55 and 48-46 kDa. Poypeptide cluster of 62-66 kDa was found common to serum samples of bovines and equines from all agro-climatic Zones. Animal trypanosomosis was found to be highly prevalent in livestock of Himachal Pradesh and thus there is dire need for designing of proper control strategies against surra.

show abstract

Section: Discussionsupporting

confidence: 84%

Sero-prevalence and immunological characterization of Trypanosoma evansi infection in livestock of four agro-climatic Zones of Himachal Pradesh, India

Sharma

Gupta

Sethi

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Major polypeptides recognized by experimental animals in our study were those with 74 to 48-46 kDa and consequently within the molecular weight range of surface antigens. Recently, Laha and Sasmal, 2008 showed 11 immunogenic proteins using hyperimmune sera from buffalo, horse and cattle, but in naturally T. evansi infected immune sera of horse detected 19 immunogenic proteins. Five common immunogenic proteins of relative molecular weight ranged 61-64, 44-47, 33-34, 25-26 and 14-16 kDa using hyperimmune and immune sera.…”

Section: Discussionmentioning

confidence: 99%

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

View full text Add to dashboard Cite

The present research investigated the presence of T. evansi antibodies in animals from the subregion of Nhecolandia, in the Pantanal Sul-mato-grossense, by means of an enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT), and the pattern of polypeptide recognition by sera from experimentally and naturally infected hosts using Western blotting. Serum samples were obtained from bovines (n = 102), horses (n = 98), and dogs (n = 55), and from 32 free-ranging coatis (Nasua nasua). None of the bovines were found positive, while sera from 16 dogs (29%) and 23 horses (23.4%) were positive by ELISA. Sera from 8 coatis (25%) were found positive using IFAT. Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa. The polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. The 48-46 and 38 kDa bands were mainly recognized in chronic phase of infection. The antigen with apparent molecular weight of 66 kDa, revealed by antibodies from all experimental animals, was also recognized in sera of horses and dogs from the Pantanal. The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and must be further evaluated for use in specific diagnosis of T. evansi infection.Keywords: Trypanosoma evansi, ELISA-test, IFAT, Pantanal mato-grossense, antigenic characterization. ResumoO trabalho de pesquisa investigou a presença de anticorpos anti-T. evansi em animais da sub-região da Nhecolândia, no Pantanal sul-mato-grossense, pelo ensaio imunoenzimático (ELISA) e a reação de imunofluorescência indireta (RIFI). O reconhecimento de polipeptídeos de T. evansi foi realizado pela técnica de "Western blotting", utilizando soros de animais experimentalmente e naturalmente infectados. As amostras de soro foram obtidas de bovinos (n = 102), cavalos (n = 98) e cães (n = 55) e de 32 quatis de vida livre (Nasua nasua) do pantanal mato-grossense. Todos os soros dos bovinos foram negativos, enquanto soros de 16 cães (29%) e 23 cavalos (23,4%) foram positivos pelo ELISA. Soros de oito quatis (25%) foram positivos pela RIFI. Pelo "Western-blotting" foi possível revelar polipeptídeos de T. evansi, com peso molecular variando de 74 a 38 kDa. Os polipeptídeos de 66, 48-46 e 38 kDa foram identificados por soros de bovinos, cavalos, cães e quatis experimentalmente infectados. As bandas de 48-46 e 38 kDa foram reconhecidas principalmente na fase crônica da infecção. O antígeno com peso molecular aparente de 66 kDa, revelado por anticorpos de todos os animais experimentais, também foi reconhecido por soros de cavalos e cães do Pantanal. O polipeptídeo de 48-46 kDa foi identificado por anticorpos de todos os animais naturalmente infectados, devendo ser avaliado para o diagnóstico específico da infecção pelo T. evansi.

show abstract

“…Serological agglutination tests have been successfully used for both diagnostic and serum epidemiological studies of numerous diseases in several animal species ( Laha & Sasmal, 2008 ; Singla et al., 2015 ). Rapid serological tests can be more practical as they enable screening large numbers of animals promptly and at a low cost.…”

Section: Introductionmentioning

confidence: 99%

“…Crude sonicated antigens of T. evansi isolates from different host origins revealed a similar polypeptide profile, and whole cell lysate (WCL) antigens prepared from T. evansi isolated from a unique host might be useful for serodiagnosis of trypanosomiasis in several species of animals ( Laha & Sasmal, 2008 ; Sivajothi et al., 2016 ). High levels of specificity for serum agglutination tests can be obtained by acidic buffering of the antigen (pH ∼3.65), which decreases the activity of agglutinins with less specificity, such as IgM, thus favoring reactions based on IgG ( Corbel, 1972 ; Patterson et al., 1976 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil

Reck

Menin

Batista

et al. 2021

Current Research in Parasitology & Vector-Borne Diseases

View full text Add to dashboard Cite

Characterization of immunogenic proteins of Trypanosoma evansi isolated from three different Indian hosts using hyperimmune sera and immune sera

Cited by 10 publications

References 12 publications

Sero-prevalence and immunological characterization of Trypanosoma evansi infection in livestock of four agro-climatic Zones of Himachal Pradesh, India

Sero-prevalence and immunological characterization of Trypanosoma evansi infection in livestock of four agro-climatic Zones of Himachal Pradesh, India

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Evaluation of buffered Trypanosoma evansi antigen and rapid serum agglutination test (BA/Te) for the detection of anti-T. evansi antibodies in horses in Brazil

Contact Info

Product

Resources

About