Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)

Harjula, Sanna-Kaisa E.; Saralahti, Anni; Ojanen, Markus; Rantapero, Tommi; Uusi-Mäkelä, Meri; Nykter, Matti; Lohi, Olli; Parikka, Mataleena; Rämet, Mika

doi:10.1016/j.dci.2019.103523

Cited by 13 publications

(14 citation statements)

References 117 publications

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“…Animal models are another bottleneck to the translation of new technology. M. tuberculosis has been studied in various preclinical models, including zebrafish, mice, rabbits, guinea pigs, and nonhuman primates, infected with model organisms (37,(131)(132)(133)(134)(135)(136). The sensitivity to infection, immune responses to the pathogens, and the type of lesions vary with animal models; thus, the choice of animal models depends on the purpose of research (131).…”

Section: Discussionmentioning

confidence: 99%

Meta-Analysis of Drug Delivery Approaches for Treating Intracellular Infections

2022

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This meta-analysis aims to evaluate the trend, methodological quality and completeness of studies on intracellular delivery of antimicrobial agents. PubMed, Embase, and reference lists of related reviews were searched to identify original articles that evaluated carrier-mediated intracellular delivery and pharmacodynamics (PD) of antimicrobial therapeutics against intracellular pathogens in vitro and/or in vivo. A total of 99 studies were included in the analysis. The most commonly targeted intracellular pathogens were bacteria (62.6%), followed by viruses (16.2%) and parasites (15.2%). Twenty-one out of 99 (21.2%) studies performed neither microscopic imaging nor flow cytometric analysis to verify that the carrier particles are present in the infected cells. Only 31.3% of studies provided comparative inhibitory concentrations against a free drug control. Approximately 8% of studies, albeit claimed for intracellular delivery of antimicrobial therapeutics, did not provide any experimental data such as microscopic imaging, flow cytometry, and in vitro PD. Future research on intracellular delivery of antimicrobial agents needs to improve the methodological quality and completeness of supporting data in order to facilitate clinical translation of intracellular delivery platforms for antimicrobial therapeutics.

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Section: Discussionmentioning

confidence: 99%

Meta-Analysis of Drug Delivery Approaches for Treating Intracellular Infections

2022

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“…For determination of the host immune response, kidney, the main hematopoietic organ of adult zebrafish, was isolated after 2 weeks of infection ( 46 ). The sacrificed zebrafish were dissected and the kidneys attached to the spinal column were removed.…”

Section: Methodsmentioning

confidence: 99%

WhiB4 Is Required for the Reactivation of Persistent Infection of Mycobacterium marinum in Zebrafish

et al. 2022

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“…Those authors proved that the M. marinum infection model in adult zebrafish was suitable for preclinical screening of tuberculosis immune's responses and vaccines. It was also a promising new model for tuberculosis vaccine research, including the pre-clinical identification of vaccine antigens [16,17,36,41,61,67];). Other species of Mycobacterium have also been studied, such as M. bovis [52,73] and M. abscessos [7].…”

Section: Animals and Human Vaccinesmentioning

confidence: 99%

Zebrafish as an alternative animal model in human and animal vaccination research

et al. 2020

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Much of medical research relies on animal models to deepen knowledge of the causes of animal and human diseases, as well as to enable the development of innovative therapies. Despite rodents being the most widely used research model worldwide, in recent decades, the use of the zebrafish (Danio rerio) model has exponentially been adopted among the scientific community. This is because such a small tropical freshwater teleost fish has crucial genetic, anatomical and physiological homology with mammals. Therefore, zebrafish constitutes an excellent experimental model for behavioral, genetic and toxicological studies which unravels the mechanism of various human diseases. Furthermore, it serves well to test new therapeutic agents, such as the safety of new vaccines. The aim of this review was to provide a systematic literature review on the most recent studies carried out on the topic. It presents numerous advantages of this type of animal model in tests of efficacy and safety of both animal and human vaccines, thus highlighting gains in time and cost reduction of research and analyzes.

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Characterization of immune response against Mycobacterium marinum infection in the main hematopoietic organ of adult zebrafish (Danio rerio)

Cited by 13 publications

References 117 publications

Meta-Analysis of Drug Delivery Approaches for Treating Intracellular Infections

Meta-Analysis of Drug Delivery Approaches for Treating Intracellular Infections

WhiB4 Is Required for the Reactivation of Persistent Infection of Mycobacterium marinum in Zebrafish

Zebrafish as an alternative animal model in human and animal vaccination research

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