Characterization of imipenem-resistant Serratia marcescens producing IMP-type and TEM-type β-lactamases encoded on a single plasmid

Zhao, Weihua; Hu, Zhiqing; Chen, Gelin; Matsushita, Kouichiro; Fukuchi, Kunihiko; Shimamura, Tadakatsu

doi:10.1016/j.micres.2006.06.005

Cited by 12 publications

(4 citation statements)

References 26 publications

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“…On the other, other beta-lactamases, including CTX-M3, IMP-type, as well as TEMtype have also been identified in Serratia spp. 14,[46][47][48] Specifically, the issue of the emergence of carbapenem resistance Serratia strains is of major importance. In our study, both imipenem and meropenem exhibited high antimicrobial activity rates against the tested Serratia marcescens and non-marcescens isolates.…”

Section: Discussionmentioning

confidence: 99%

Serratia infections in a general hospital: characteristics and outcomes

Samonis

Vouloumanou

Christofaki

et al. 2011

Eur J Clin Microbiol Infect Dis

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We aimed to present our experience regarding infections caused by Serratia spp. in a region with relatively high antimicrobial resistance rates. We retrospectively reviewed the databases of the microbiological laboratory of the University Hospital of Heraklion, Crete (2/2004-12/2009). A total of 77 patients [67.5% men, mean age ± standard deviation (SD) = 56.9 ± 24.5 years) were identified; 37.7% were outpatients. Sixty-five (84.4%) of the 77 included patients had a Serratia marcescens isolate; the remaining 12 patients had a non-marcescens Serratia spp. The most frequently observed infections were respiratory tract infection (32.5%) and keratitis/endophthalmitis (20.8%). Seventy-three (94.9%) patients were cured. Four deaths were observed; three of them were considered as attributed to the Serratia infection. No difference was found regarding the characteristics and outcomes between patients with Serratia marcescens and non-marcescens infections. In addition, antipseudomonal penicillins and their combinations with beta-lactamase inhibitors, as well as carbapenemes, and fluoroquinolones exhibited high antimicrobial activity against both the tested Serratia marcescens and non-marcescens isolates. Our study adds useful information regarding the characteristics and outcomes of patients with Serratia infection, as well as the susceptibilities of the respective Serratia marcescens and non-marcescens isolates, in a region with relatively high levels of antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Serratia infections in a general hospital: characteristics and outcomes

Samonis

Vouloumanou

Christofaki

et al. 2011

Eur J Clin Microbiol Infect Dis

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“…The majority of these genes exhibited a high percentage identity with genes from various members of the Proteobacteria including E. coli , Klebsiella , Salmonella , Serratia, Vibrio parahaemolyticus and Escherichia vulneris . The resistance of strains of Salmonella and Serratia to β-lactams via bla TEM genes has been noted [33-35] and such strains have been associated with nosocomial infections [36]. In contrast, there have been relatively few studies of bla TEM genes in Vibrio parahaemolyticus and Escherichia vulneris [37,38].…”

Section: Resultsmentioning

confidence: 99%

A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota

et al. 2014

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BackgroundThe potential for the human gut microbiota to serve as a reservoir for antibiotic resistance genes has been the subject of recent discussion. However, this has yet to be investigated using a rapid PCR-based approach. In light of this, here we aim to determine if degenerate PCR primers can detect aminoglycoside and β-lactam resistance genes in the gut microbiota of healthy adults, without the need for an initial culture-based screen for resistant isolates. In doing so, we would determine if the gut microbiota of healthy adults, lacking recent antibiotic exposure, is a reservoir for resistance genes.ResultsThe strategy employed resulted in the identification of numerous aminoglycoside (acetylation, adenylation and phosphorylation) and β-lactam (including blaOXA, blaTEM, blaSHV and blaCTX-M) resistance gene homologues. On the basis of homology, it would appear that these genes originated from different bacterial taxa, with members of the Enterobacteriaceae being a particularly rich source. The results demonstrate that, even in the absence of recent antibiotic exposure, the human gut microbiota is a considerable reservoir for antibiotic resistance genes.ConclusionsThis study has demonstrated that the gut can be a significant source of aminoglycoside and β-lactam resistance genes, even in the absence of recent antibiotic exposure. The results also demonstrate that PCR-based approaches can be successfully applied to detect antibiotic resistance genes in the human gut microbiota, without the need to isolate resistant strains. This approach could also be used to rapidly screen other complex environments for target genes.

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“…However, the emergence of carbapenem-hydrolyzing metallo-␤ -lactamases (MBLs) is seriously threatening the clinical medication of carbapenems [1][2][3] . Of the identified MBLs including IMP, VIM, SPM-1 and GIM-1, the IMP-type MBLs are the most common [4,5] .…”

Section: Introductionmentioning

confidence: 99%

Contributions of IMP-10 Metallo-β-Lactamase, the Outer Membrane Barrier and the MexAB-OprM Efflux System to High-Level Carbapenem Resistance in <i>Pseudomonas aeruginosa</i>

Zhao

Hu²,

Chen³

et al. 2009

Chemotherapy

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Background: The emergence of carbapenem-hydrolyzing metallo-β-lactamases (MBLs) is a serious threat to clinical medication of carbapenems. We evaluated the contributions of IMP-10 MBL, the outer membrane barrier and the MexAB-OprM efflux system to high-level carbapenem resistance in Pseudomonas aeruginosa clinical isolates. Methods: MBL-producing strains were screened by ceftazidime and mercaptoacetic acid double-disk synergy testing. Genes were determined by PCR analysis and DNA sequencing. IMP-10 MBL activity was assayed using nitrocefin as a substrate. Results: The bla_IMP-10+ strains showed high-level resistance to carbapenems, with minimum inhibitory concentrations of 512 to >4,096 μg/ml. The minimum inhibitory concentrations were decreased by the inhibitors of MBLs, sodium mercaptoacetate (SMA) and ethylenediaminetetraacetic acid (EDTA), 16 and 64 fold, respectively. However, against the activity of the prepared IMP-10 MBL, the 50% inhibitory concentrations of SMA and EDTA were 5.5 and 211.6 μM, respectively, indicating that SMA was much more effective than EDTA in blocking the enzyme activity. The contradictory results may be explained by the additional effect of EDTA as an outer membrane permeabilizer because the susceptibility of the bla_IMP– strains to carbapenems was increased 2 fold by EDTA, but not by SMA. In the presence of carbonyl cyanide m-chlorophenylhydrazone, a proton motive force inhibitor, the susceptibility of the P. aeruginosa isolates to carbapenems was increased 1–4 fold. Conclusion: The acquired IMP-10 MBL is a crucial factor in the high-level resistance to carbapenems in P. aeruginosa clinical isolates, while the outer membrane barrier and the MexAB-OprM efflux system play a basic and minor role, respectively.

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Characterization of imipenem-resistant Serratia marcescens producing IMP-type and TEM-type β-lactamases encoded on a single plasmid

Cited by 12 publications

References 26 publications

Serratia infections in a general hospital: characteristics and outcomes

Serratia infections in a general hospital: characteristics and outcomes

A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota

Contributions of IMP-10 Metallo-β-Lactamase, the Outer Membrane Barrier and the MexAB-OprM Efflux System to High-Level Carbapenem Resistance in <i>Pseudomonas aeruginosa</i>

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