Characterization of Nocardia farcinica, a Filamentous Bacterium Isolated from Foaming Activated Sludge Samples

Abstract

The identification and ch;iracterization of filamentous bacteria and their association with specific plant operating conditions and influent characteristics has been hampered because of morphological variations and differences between process configurations. A study was conducted to isolate and characterize the predominant filamentous bacteria observed in a foaming activated sludge treatment plant. The predominant foam-forming filament was isolated and characterized using microscopic, biochemical and molecular techniques. The phylogenetic analysis of the 16S rRNA gene confirmed that it was Nocardia farcinica, a typical filamentous foam-foaming pathogenic bacterium which is not widely reported outside of South Africa. The bacterium used a variety of substrates for its growth and showed greater affinity to larger and slowly biodegradable compounds. The N. farcinica grew well at temperatures ranging from 12 to 30 C in R2A medium and with a pH ranging from 5.5 to 8.0 and an NaCl concentration of 1 to 5%. This range of conditions shows that A' , farcinica can withstand extreme conditions, which results in its proliferation in foaming samples. Water Environ. Res., 83, 527 (2011).

Comparison of three phenotypic and deoxyribonucleic acid extraction methods for isolation and Identification of Nocardia spp

“…In further studies, we must do molecular tests according to 16S rDNA sequencing, for precisely identification of Nocardia species. [13293031]…”