Characterization of
            <i>Listeria monocytogenes</i>
            Strains Involved in Invasive and Noninvasive Listeriosis Outbreaks by PCR-Based Fingerprinting Techniques

Franciosa, Giovanna; Tartaro, S; Wedell-Neergaard, Christina; Aureli, Paolo

doi:10.1128/aem.67.4.1793-1799.2001

Cited by 39 publications

(23 citation statements)

References 29 publications

(26 reference statements)

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“…2; Table 3) and distinguished the most common virulent serotypic group, 4b, from the other serotypic groups of lineage I. Similar differentiation was achieved using infrequent restriction site PCR (16). Furthermore, similar to the AILP analysis, only 4 probes (out of 29) of the mixed-genome microarray were needed for the same differentiation (10).…”

Section: Vol 71 2005 Ailp a Pcr-based Bacterial Classification 3149mentioning

confidence: 68%

Amplified Intergenic Locus Polymorphism as a Basis for Bacterial Typing of Listeria spp. and Escherichia coli

Somer

Danin‐Poleg

Diamant

et al. 2005

Appl Environ Microbiol

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DNA-based methods are increasingly important for bacterial typing. The high number of polymorphic sites present among closely related bacterial genomes is the basis for the presented method. The method identifies multilocus genomic polymorphisms in intergenic regions termed AILP (amplified intergenic locus polymorphism). For each locus, a pair of unique PCR primers was designed to amplify an intergenic sequence from one open reading frame (ORF) to the adjacent ORF. Presence, absence, and size variation of the amplification products were identified and used as genetic markers for rapidly differentiating among strains. Polymorphism was evaluated using 18 AILP sites among 28 strains of Listeria monocytogenes and 6 strains of Listeria spp. and 30 AILP markers among 27 strains of Escherichia coli. Up to four alleles per locus were identified among Listeria strains, and up to six were identified among E. coli strains. In both species, more than half of the AILP sites revealed intraspecies polymorphism. The AILP data were applied to phylogenetic analysis among Listeria and E. coli strains. A clear distinction between L. monocytogenes and Listeria spp. was demonstrated. In addition, the method separated L. monocytogenes into the three known lineages and discriminated the most common virulent serotypic group, 4b. In E. coli, AILP analysis separated the known groups as well as the virulent O157:H7 isolates. These findings for both Listeria and E. coli are in agreement with other phylogenetic studies using molecular markers. The AILP method was found to be rapid, simple, reproducible, and a low-cost method for initial bacterial typing that could serve as a basis for epidemiological investigation.Bacterial strain typing has several important applications in microbiology. In clinical practice, strain typing is useful for diagnosis and determining treatment strategy and is essential for rapid identification of disease outbreaks and new virulent strains. In the food industry, strain typing is necessary to ensure food safety and for linking cases of food-borne infections to suspected items in the food chain. Classical bacterial identification is based on selective enrichment, followed by plating on selective media. Species identification is mainly by biochemical characterization, and strain identification is primarily based on serology. These methods do not meet the requirement for rapid identification and typing in clinical, epidemiological, and food industry applications. Recent advances in biotechnology have resulted in the development of numerous methods for detection and typing of microorganisms (11-13, 19, 25) which differ in their sensitivity, rapidity, labor intensiveness, complexity, discriminatory power, reproducibility, and cost (5, 32, 43, 49). In principle, by screening a large number of polymorphic sites, genomic methods should be able to provide very accurate discrimination among closely related strains. The total multilocus output of these methods is often termed "DNA fingerprints" or a "DNA bar code."In the pre...

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Section: Vol 71 2005 Ailp a Pcr-based Bacterial Classification 3149mentioning

confidence: 68%

Amplified Intergenic Locus Polymorphism as a Basis for Bacterial Typing of Listeria spp. and Escherichia coli

Somer

Danin‐Poleg

Diamant

et al. 2005

Appl Environ Microbiol

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“…The groups at higher risk of contracting the disease are young people, the old, (over 65), pregnant women, and immune compromised people, the YOPI, an acronym coined by De Cesare et al [3, 4]. In healthy subjects, L. monocytogenes can lead to episodes of gastroenteritis and fever [5, 6]. …”

Section: Introductionmentioning

confidence: 99%

Listeria monocytogenesin Ready-to-Eat Seafood and Potential Hazards for the Consumers

Gambarin

Magnabosco

Losio

et al. 2012

International Journal of Microbiology

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The risk of exposure toListeria monocytogenes(L. monocytogenes) when consuming Ready-to-Eat (RTE) seafood was assessed in the Veneto Region (Italy). Thirty-eight samples were analyzed, each sample consisted of three subunits belonging to the same batches. The first of the three units was examined immediately, the second was stored at +4°C (for all of its shelf-life) and the third at +10°C (for the latter third of itsshelf-life) before the analysis. Chemical-physical and microbiological parameters were tested simultaneously. Culture results showed the presence of viableL. monocytogenesin 9 (23,68%) of the 38 samples analysed, 3 (33,33%) of which with a concentration >100 cfu/g. PCR tests yielded 12L. monocytogenespositive samples. Semipreserves with aw (water activity) and pH values that favourL. monocytogenesgrowth were the only ones to result positive to microbiological and PCR tests. Temperature proved to be an important factor as it limits the growth ofL. monocytogenes, including products with potentially high competitive microbial charges. Four different serotypes were recovered and ribotyping has helped to highlight the genomic variability ofL. monocytogenesstrains in food. This supports the hypothesis thatL. monocytogenescontinues to evolve genetically to the detriment of phenotypic conservation.

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“…Whether the capability of survival and growth is an attribute of a particular clone may be shown by subtyping. Typing could also be useful for establishing the association of a certain microbial type with specific traits of virulence or pathogenicity [2].…”

Section: Introductionmentioning

confidence: 99%

Characterization ofListeria monocytogenesrecovered from 41 cases of sporadic listeriosis in Austria by serotyping and pulsed-field gel electrophoresis

Wagner

Allerberger²

2003

FEMS Immunology &Amp; Medical Microbiology

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41 clinical Listeria monocytogenes strains recovered from seven feto-maternal and 34 non-pregnancy associated cases of human listeriosis documented between 1997 and 2000 underwent serotyping and typing by pulsed-field gel electrophoresis (PFGE) applying the enzymes AscI, ApaI and SmaI. The pulsotypes of the clinical strains were compared to the pulsotypes of three L. monocytogenes strains isolated from healthy fecal carriers and nine reference strains isolated from seven outbreaks in Europe and the USA. The 41 clinical strains of Austrian provenance showed 37 pulsotypes. Five sets of two Austrian strains each were indistinguishable by PFGE typing. Epidemiological links were absent between these indistinguishable isolates. One unique pulsotype (AB) was found in three fecal isolates. Five pulsotypes (A, Q, R, AC and AD) were distinguished among the strains associated with outbreaks. Clusters consisting of two, five and six Austrian strains each were indistinguishable from the outbreak-associated pulsotypes A, Q and R, respectively, after PFGE analysis with AscI. Three strains of AscI pulsotype Q and five strains of AscI pulsotype R could be further differentiated by restriction with ApaI and SmaI. One strain each from sporadic cases shared a combined pulsotype with the outbreak strains of pulsotypes A and R, respectively. These PFGE data suggest that a similar genetic background can be found in strains which have been contributing to outbreaks world-wide and in isolates associated with sporadic listeriosis in Austria.

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Characterization of Listeria monocytogenes Strains Involved in Invasive and Noninvasive Listeriosis Outbreaks by PCR-Based Fingerprinting Techniques

Cited by 39 publications

References 29 publications

Amplified Intergenic Locus Polymorphism as a Basis for Bacterial Typing of Listeria spp. and Escherichia coli

Amplified Intergenic Locus Polymorphism as a Basis for Bacterial Typing of Listeria spp. and Escherichia coli

Listeria monocytogenesin Ready-to-Eat Seafood and Potential Hazards for the Consumers

Characterization ofListeria monocytogenesrecovered from 41 cases of sporadic listeriosis in Austria by serotyping and pulsed-field gel electrophoresis

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