Characterization of<i>Listeria monocytogenes</i>recovered from 41 cases of sporadic listeriosis in Austria by serotyping and pulsed-field gel electrophoresis

Wagner, Martin; Allerberger, Franz

doi:10.1016/s0928-8244(02)00445-5

Cited by 40 publications

(22 citation statements)

References 36 publications

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“…In Austria, serovar 4b accounts for 63.4% of the reported human listeriosis cases (Wagner and Allerberger, 2002). This predominance of serovar 4b strains is comparable to that observed in other countries.…”

Section: Introductionsupporting

confidence: 59%

A Comparison ofListeria monocytogenesSerovar 4b Isolates of Clinical and Food Origin in Austria by Automated Ribotyping and Pulsed-Field Gel Electrophoresis

Grif¹,

Heller²,

Wagner³

et al. 2006

Foodborne Pathogens and Disease

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In this study, two typing methods, automated ribotyping and pulsed-field gel electrophoresis (PFGE), were evaluated for the subtyping of Listeria monocytogenes serotype 4b. The strains originated from patients and food samples collected in Austria during 2001-2005 and from Europe and North America in the World Health Organization collaborative study on the subtyping of this species. The largest group of Austrian clinical isolates was of the same PFGE subtype as those isolated from foodborne outbreaks in Switzerland and in the United States. Another subtype of clinical isolates from Austria was indistinguishable to that obtained from isolates responsible for a foodborne outbreak in the United States in 1985. Although the discriminatory power of PFGE was higher than that of automated ribotyping, some PFGE types were differentiated by ribotyping. Thus, combining data obtained by both automated ribotyping and PFGE increases the strain discrimination. Still, many of the Austrian strains remain indistinguishable from strains of foodborne outbreaks in other countries although there is no known epidemiological relation. This complies to previous studies which show the highly clonal nature of L. monocytogenes 4b strains which are responsible for both large outbreaks and sporadic cases.

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Section: Introductionsupporting

confidence: 59%

A Comparison ofListeria monocytogenesSerovar 4b Isolates of Clinical and Food Origin in Austria by Automated Ribotyping and Pulsed-Field Gel Electrophoresis

Grif¹,

Heller²,

Wagner³

et al. 2006

Foodborne Pathogens and Disease

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“…For instance, in a study that included 41 clinical isolates obtained from sporadic cases of listeriosis in Austria between 1997 and 2000 the workers found 37 unique pulsotypes (33). In another study the workers examined 48 clinical isolates from sporadic listeriosis cases in Belgium in 2001 and found 34 different AscI profiles and 38 different ApaI profiles, providing additional support for the hypothesis that listeriosis involves a variety of genetically distinct strains (37).…”

Section: Discussionmentioning

confidence: 95%

Relatedness of Listeria monocytogenes Isolates Recovered from Selected Ready-To-Eat Foods and Listeriosis Patients in the United States

Gilbreth

Call

Wallace

et al. 2005

Appl Environ Microbiol

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Pulsed-field gel electrophoresis and serotyping were performed for 544 isolates of Listeria monocytogenes, including 502 isolates recovered from contaminated samples from 31,705 retail ready-to-eat (RTE) food products and 42 isolates recovered from human cases of listeriosis. The isolates were from Maryland (294 isolates) and California (250 isolates) and were collected in 2000 and 2001. The isolates were placed into 16 AscI pulsogroups (level of relatedness within each group, >66%), 139 AscI pulsotypes (levels of relatedness, >25% to 100%), and eight serotypes (serotypes 1/2a, 1/2b, 1/2c, 3a, 3b, 4b, 4c, and 4d). The most frequently found pulsotypes belonged to either pulsogroup A (150 food isolates plus 4 clinical isolates) or pulsogroup B (104 food isolates plus 5 clinical isolates). The majority of the 502 food isolates were either serotype 1/2a (298 isolates) or serotype 1/2b (133 isolates), whereas the majority of the 42 clinical isolates were either serotype 1/2a (19 isolates) or serotype 4b (15 isolates). Additionally, 13 clinical isolates displayed pulsotypes also found in food isolates, whereas the remaining 29 clinical isolates displayed 24 unique pulsotypes. These data indicate that most (86%) of the L. monocytogenes subtypes found in the RTE foods sampled belonged to only two serotypes and that 90% of the isolates displayed 73 pulsotypes, with 107 isolates displaying pulsotype 1. These data should help define the distribution and relatedness of isolates found in RTE foods in comparison with isolates that cause listeriosis.

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“…Such testing permits specific interventional measures to prevent further cases of 'food poisoning' [20,25,26]. An article on an outbreak of febrile gastro enteritis associated with yellied pork contaminated with L. monocytogenes in Austria in 2008, published in this issue of Wiener klinische Wochenschrift shows the great potential of epidemiological outbreak investiga tions in recognizing sources of infections due to L. monocytogenes and their transmission routes [27].…”

Section: Month Casesmentioning

confidence: 99%

Epidemiology of listeriosis in Austria

Kasper

Huhulescu

Auer

et al. 2009

Wien Klin Wochenschr

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A total of 150 human cases of listeriosis (case definition based on isolation of Listeria monocytogenes from normally sterile material) were reported in Austria between 1997 and 2007. Of these, 14 cases (9.3%) were pregnancy-associated (mother/child illness considered as a single case) with a mean age of 29.3 years (median: 26.5; range 24-36). Among the non-pregnancy-associated cases (n = 136), 75 were male (55.2%) and 61 female (44.9%); patients in this group had a mean age of 64.3 years (median: 66.2; range 1-93). The average incidence of listeriosis in Austria in the period studied was 0.168 cases per 100,000 population. The majority of cases (90.7%) were caused by systemic infection, only 9.3% of cases were local infections. Among non-pregnancy-associated cases the fatality rate was 28.7% (39/136) and among the pregnancy-associated cases 35.7% (5/14: miscarriage x3, stillbirth x1, and one death in a newborn within 15 days of birth). Serotyping results for the 150 isolates revealed serovar (SV) 4b: 54%, SV 1/2a: 31.3%, SV 1/2b: 10%, SV 1/2c: 2.7%, 4d: 1.3% and SV 3a: 0.7%. Predisposing risk factors were determined for 131 of the 150 cases: age > or = 65 years (n = 73), pregnancy (n = 14) and 44 cases of carcinoma, blood malignancies, autoimmune diseases and status post solid organ transplants (7 patients had more than one underlying illness). During the period studied, the incidence of listeriosis doubled, despite a drastic reduction in the frequency of pregnancy-associated cases.

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Characterization ofListeria monocytogenesrecovered from 41 cases of sporadic listeriosis in Austria by serotyping and pulsed-field gel electrophoresis

Cited by 40 publications

References 36 publications

A Comparison ofListeria monocytogenesSerovar 4b Isolates of Clinical and Food Origin in Austria by Automated Ribotyping and Pulsed-Field Gel Electrophoresis

A Comparison ofListeria monocytogenesSerovar 4b Isolates of Clinical and Food Origin in Austria by Automated Ribotyping and Pulsed-Field Gel Electrophoresis

Relatedness of Listeria monocytogenes Isolates Recovered from Selected Ready-To-Eat Foods and Listeriosis Patients in the United States

Epidemiology of listeriosis in Austria

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