Characterization of<i>Candida</i>antigens by crossed-immunoaffinoelectrophoresis

Bruneau, S.M.; Guinet, R

doi:10.1080/00362178585380181

Cited by 8 publications

(3 citation statements)

References 16 publications

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“…Previous studies mentioned species-specific C. albicans components (6,7,16,22), but they were not present on the blastospore surface and required a more or less complex extraction…”

Section: Discussionmentioning

confidence: 99%

Evaluation of latex reagents for rapid identification of Candida albicans and C. krusei colonies

et al. 1997

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A total of 322 yeast strains and yeastlike organisms belonging to the genera Candida, Cryptococcus, Geotrichum, Saccharomyces, and Trichosporon were tested with the new monoclonal antibody-based Bichro-latex albicans and Krusei color latex tests. Comparison of results with those obtained by conventional identification methods showed 100% sensitivity for both latex tests and 100% and 95% specificity for the Bichro-latex albicans and Krusei color tests, respectively. Because the test is easy to read and quick to perform, the Bichro-latex albicans test may be useful for rapid identification of Candida albicans colonies in the clinical laboratory.

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“…Previous studies mentioned species-specific C. albicans components (6,7,16,22), but they were not present on the blastospore surface and required a more or less complex extraction…”

Section: Discussionmentioning

confidence: 99%

Evaluation of latex reagents for rapid identification of Candida albicans and C. krusei colonies

et al. 1997

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“…Initially gels prepared in the laboratory were used, later followed by commercially available ready-touse gels, usually affording better reproducibility. Specific detection methods were applied for the visualization of enzymes [30,31,70]. Only few authors in the field of natural history have used other techniques, such as twodimensional gel electrophoresis [32,34], PAGE [2,6,14,33], titration curves, found particularly useful for bacterial taxonomy [35,36], as well as blotting and immunoblotting techniques [37,38].…”

Section: Historical Summarymentioning

confidence: 99%

The use of polyacrylamide gel electrophoresis in animal systematics, phylogeny, and ecophysiology

D'hondt

2001

Electrophoresis

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In the 1980s, the use of polyacrylamide gel electrophoresis (PAGE) was popular. But more recent developments in other electrophoretic techniques have resulted in this method being less widely used. However, it is adequate for the needs of naturalists for issues which do not require high-performance methods, such as in systematics, phylogeny, studies of intraspecific or clinal variability, ecology and ecophysiology. We illustrate the application of PAGE by the results of a research program on marine and terrestrial invertebrates which was conducted from 1979 to 1992, but which is still used to initiate graduate students to the research. Thus, issues faced by the zoologist can be clarified by this method, not yet obsolete and still useful in natural history despite considerable advances in other electrophoretic methods.

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“…The aim of this study was to characterize the interactions between Cundida extracts and fibrinogen in our standardized quantitative immunoelectrophoreses (7) by the use of a modification of the immunoaffinoelectrophoresis introduced by Bog-Hansen (1) and thus to pursue the characterization of Cundidu antigens (5,6).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Fibrinogen Binding Factor from Candida albicans and Candida tropicalis in Quantitative Immunoelectrophoresis

Guinet

Bruneau

1988

Mycoses

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Summary: The fibrinogen binding factor (FBF) was characterized in Candida albicans and Candida tropicalis reference systems by crossed immunoaffinoelectrophoresis (CIAE) with free fibrinogen in the first dimension gel. The FBF of Candida albicans and Candida tropicalis showed the same mobility and reactivity and corresponded to a cross reactive glycoproteic antigen. Thus CIAE provides a useful tool for the identification of biologically active antigens. Zusammenfassung: Der Fibrinogen‐bindende Faktor (FBF) wurde in Candida albicans‐ und Candida tropicalis‐Referenz‐systemen mittels gekreuzter Immunoaffinoelektrophorese (CIAE) mit freien Fibrinogen in der ersten Dimension charakterisiert. Die FBF von Candida albicans und Candida tropicalis zeigten die gleiche Motilität und Reaktivität und entsprachen einem kreuzreagierenden Glykoprotein. Die CIAE erweist sich somit als geeignetes Instrument für die Identifizierung biologisch aktiver Antigene.

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Characterization ofCandidaantigens by crossed-immunoaffinoelectrophoresis

Cited by 8 publications

References 16 publications

Evaluation of latex reagents for rapid identification of Candida albicans and C. krusei colonies

Evaluation of latex reagents for rapid identification of Candida albicans and C. krusei colonies

The use of polyacrylamide gel electrophoresis in animal systematics, phylogeny, and ecophysiology

Characterization of the Fibrinogen Binding Factor from Candida albicans and Candida tropicalis in Quantitative Immunoelectrophoresis

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