Characterization of <i>Brucella</i> spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda

Ntivuguruzwa, Jean Bosco; Kolo, Francis Babaman; Mwikarago, Emil Ivan; vanHeerden, Henriette

doi:10.1002/vms3.805

Cited by 5 publications

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“…and mixed farming of cattle and goats or sheep. The mixed infection and mixed farming were reported in our study that identified both pathogens in aborting goat flock in Rwanda [ 46 ]. The co-infection of B .…”

Section: Discussionmentioning

confidence: 81%

Seroprevalence of brucellosis and molecular characterization of Brucella spp. from slaughtered cattle in Rwanda

et al. 2022

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Bovine brucellosis is endemic in Rwanda, although, there is a paucity of documented evidence about the disease in slaughtered cattle. A cross-sectional study was conducted in slaughtered cattle (n = 300) to determine the seroprevalence of anti-Brucella antibodies using the Rose Bengal Test (RBT), and indirect enzyme-linked immunosorbent assay (i-ELISA). Corresponding tissues were cultured onto a modified Centro de Investigación y Tecnología Agroalimentaria (CITA) selective medium and analysed for Brucella spp. using the 16S-23S ribosomal interspacer region (ITS), AMOS, and Bruce-ladder PCR assays. The seroprevalence was 20.7% (62/300) with RBT, 2.9% (8/300) with i-ELISA, and 2.9% (8/300) using both tests in series. Brucella-specific 16S-23S ribosomal DNA interspace region (ITS) PCR detected Brucella DNA in 5.6% (17/300; Brucella culture prevalence). AMOS-PCR assay identified mixed B. abortus and B. melitensis (n = 3), B. abortus (n = 3) and B. melitensis (n = 5) while Bruce-ladder PCR also identified B. abortus (n = 5) and B. melitensis (n = 6). The gold standard culture method combined with PCR confirmation identified 5.6% Brucella cultures and this culture prevalence is higher than the more sensitive seroprevalence of 2.9%. This emphasizes the need to validate the serological tests in Rwanda. The mixed infection caused by B. abortus and B. melitensis in slaughtered cattle indicates cross-infection and poses a risk of exposure potential to abattoir workers. It is essential to urgently strengthen a coordinated national bovine brucellosis vaccination and initiate a test-and-slaughter program that is not presently applicable in Rwanda.

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Section: Discussionmentioning

confidence: 81%

Seroprevalence of brucellosis and molecular characterization of Brucella spp. from slaughtered cattle in Rwanda

et al. 2022

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“…AMOS-PCR detected a mixed infection of B. abortus and B. melitensis in the blood, milk, and vaginal swabs of cattle. Mixed infections of B. abortus and B. melitensis have been recently reported in aborted tissues of goats in Rwanda ( 26 ) and in herds where cattle graze together with small ruminants in South Africa and Kenya ( 56 , 57 ). Keeping different animal species such as cattle and small ruminants on the same farm represents a risk of transmission of brucellosis to other animal species including humans.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Brucella spp. from seropositive herds of cattle farmed at the wildlife–livestock–human interface in Rwanda

et al. 2022

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Seroprevalence studies showed that brucellosis is prevalent in cattle in Rwanda with no recent study on the characterization of Brucella spp. Therefore, this study aimed to characterize Brucella spp. in seropositive herds of cattle farmed at the wildlife–livestock–human interface. Whole blood samples (n = 118), milk (n = 41), and vaginal swabs (n = 51) were collected from 64 seropositive herds. All samples (n = 210) were inoculated onto modified Centro de Investigacion y Tecnologia Agroalimentaria (CITA) selective medium. Cultures were analyzed to detect Brucella spp. using 16S−23S ribosomal DNA interspacer region (ITS) PCR, the Brucella cultures were speciated using AMOS and Bruce-ladder PCR assays. Brucella spp. were detected in 16.7% (35/210) of the samples established from the samples using ITS-PCR. The AMOS PCR assay identified mixed Brucella abortus and B. melitensis (n = 6), B. abortus (n = 7), and B. melitensis (n = 1) from cultures from blood samples; mixed B. abortus and B. melitensis (n = 1) and B. abortus (n = 4) from cultures from milk samples; mixed B. abortus and B. melitensis (n = 6), B. abortus (n = 8), and B. melitensis (n = 1) from cultures from vaginal swabs. Bruce-ladder PCR assay confirmed B. abortus and B. melitensis cultures. The isolation of Brucella spp. was significantly associated with districts, with the Nyagatare district having more isolates than other districts (p = 0.01). This study identified single or mixed B. abortus and B. melitensis infections in cattle samples in Rwanda, which emphasizes the need to improve brucellosis control at the wildlife–livestock–human interface and raise the awareness of cattle keepers, abattoir workers, laboratory personnel, and consumers of cattle products.

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Characterization of Brucella spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda

Ntivuguruzwa

Kolo

Mwikarago³

et al. 2022

Veterinary Medicine & Sci

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Background Abortions cause tremendous economic losses in food‐producing animals and may lead to food insecurity. Objectives This study aimed to characterize Brucella spp. and other abortigenic pathogens from aborted tissues of cattle. Methods For cattle, aborted tissues (n = 19) were cultured, and Brucella spp. were detected using the genus‐specific 16S‐23S ribosomal DNA interspacer region (ITS) assay and speciated using Brucella abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce‐ladder PCR assays. Brucella negative samples were screened using the eight abortigenic pathogens PCR panel. Samples from an abortion outbreak that occurred within a goat tribe were included in this investigation. Sera of females (n = 8) and males (n = 2) were analyzed using the Rose Bengal Test (RBT) and indirect enzyme‐linked immunosorbent assay (i‐ELISA), while vaginal swabs (n = 3) and aborted tissues (n = 1) were cultured and characterized. Results The ITS‐PCR detected Brucella DNA in cultures from two aborted tissues of cattle (10.5%, [2/19]), which were identified as B. melitensis (n = 1), and B. abortus (n = 1) using AMOS and Bruce‐ladder PCR assays. Campylobacter fetus (n = 7) and Leptospira spp. (n = 4) including co‐infections (n = 2) of C. fetus and Leptospira spp. were identified from the Brucella negative samples of cattle. Goats (100.0%, 10/10) were brucellosis seropositive on RBT and i‐ELISA. Mixed infections caused by B. melitensis and B. abortus were isolated from the vaginal swabs (n = 3) and aborted tissues (n = 1). Discussion and conclusions This is the first identification of abortion‐associated pathogens in aborted cattle indicating the enormous financial losses and a threat to public health. It is therefore essential to include these identified pathogens in the surveillance scheme of veterinary and human services.

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Characterization of Brucella spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda

Cited by 5 publications

References 45 publications

Seroprevalence of brucellosis and molecular characterization of Brucella spp. from slaughtered cattle in Rwanda

Seroprevalence of brucellosis and molecular characterization of Brucella spp. from slaughtered cattle in Rwanda

Molecular characterization of Brucella spp. from seropositive herds of cattle farmed at the wildlife–livestock–human interface in Rwanda

Characterization of Brucella spp. and other abortigenic pathogens from aborted tissues of cattle and goats in Rwanda

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