Characterization of <i>AtSTOP1</i> Orthologous Genes in Tobacco and Other Plant Species

Ohyama, Yoshinao; Ito, Hiroki; Kobayashi, Yuriko; Ikka, Takashi; Morita, Akio; Kobayashi, Masatomo; Imaizumi, Reiji; Aoki, Takeshi; Kono, Kenji; Sakata, Yoichi; Iuchi, Satoshi; Koyama, Hiroyuki

doi:10.1104/pp.113.218958

Cited by 81 publications

(90 citation statements)

References 42 publications

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“…NtMATE and NtALS3, was reported to be involved in Al tolerance mechanism (Ohyama et al, 2013). To determine whether the increased Al tolerance of VuAAE3 overexpressing lines is associated with changes of their expression, we also compared the expression of both genes in OX-1 to that of wild-type plants.…”

Section: Overexpression Of Vuaae3 In Transgenic Tobacco Confers Al Tomentioning

confidence: 99%

An Oxalyl-CoA Synthetase Is Involved in Oxalate Degradation and Aluminum Tolerance

Lou

Fan

et al. 2016

Plant Physiol.

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Acyl Activating Enzyme3 (AAE3) was identified to be involved in the catabolism of oxalate, which is critical for seed development and defense against fungal pathogens. However, the role of AAE3 protein in abiotic stress responses is unknown. Here, we investigated the role of rice bean (Vigna umbellata) VuAAE3 in Al tolerance. Recombinant VuAAE3 protein has specific activity against oxalate, with K m = 121 6 8.2 mM and V max of 7.7 6 0.88 mmol min 21 mg 21 protein, indicating it functions as an oxalyl-CoA synthetase. VuAAE3-GFP localization suggested that this enzyme is a soluble protein with no specific subcellular localization. Quantitative reverse transcription-PCR and VuAAE3 promoter-GUS reporter analysis showed that the expression induction of VuAAE3 is mainly confined to rice bean root tips. Accumulation of oxalate was induced rapidly by Al stress in rice bean root tips, and exogenous application of oxalate resulted in the inhibition of root elongation and VuAAE3 expression induction, suggesting that oxalate accumulation is involved in Al-induced root growth inhibition. Furthermore, overexpression of VuAAE3 in tobacco (Nicotiana tabacum) resulted in the increase of Al tolerance, which was associated with the decrease of oxalate accumulation. In addition, NtMATE and NtALS3 expression showed no difference between transgenic lines and wildtype plants. Taken together, our results suggest that VuAAE3-dependent turnover of oxalate plays a critical role in Al tolerance mechanisms.

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Section: Overexpression Of Vuaae3 In Transgenic Tobacco Confers Al Tomentioning

confidence: 99%

An Oxalyl-CoA Synthetase Is Involved in Oxalate Degradation and Aluminum Tolerance

Lou

Fan

et al. 2016

Plant Physiol.

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“…In tobacco, the decreased Al tolerance of Sensitive to Proton Rhizotoxicity1 RNA i transgenic plants is related to the conferred down-regulation of Multidrug and Toxic Compound Extrusion (NtMATE) and Aluminum Sensitive3 (NtALS3; Ohyama et al, 2013). To determine if the increased Al tolerance of VuFDH over-expressing lines is associated with changes in NtMATE or NtALS3 expression, we next compared the expression of these genes in VuFDH-over-expressing line1 with that in wild-type plants.…”

Section: Vufdh Increases Al and Low Ph Tolerance By Decreasing Formatmentioning

confidence: 99%

A Formate Dehydrogenase Confers Tolerance to Aluminum and Low pH

et al. 2016

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Formate dehydrogenase (FDH) is involved in various higher plant abiotic stress responses. Here, we investigated the role of rice bean (Vigna umbellata) VuFDH in Al and low pH (H + ) tolerance. Screening of various potential substrates for the VuFDH protein demonstrated that it functions as a formate dehydrogenase. Quantitative reverse transcription-PCR and histochemical analysis showed that the expression of VuFDH is induced in rice bean root tips by Al or H + stresses. Fluorescence microscopic observation of VuFDH-GFP in transgenic Arabidopsis plants indicated that VuFDH is localized in the mitochondria. Accumulation of formate is induced by Al and H + stress in rice bean root tips, and exogenous application of formate increases internal formate content that results in the inhibition of root elongation and induction of VuFDH expression, suggesting that formate accumulation is involved in both H + -and Al-induced root growth inhibition. Over-expression of VuFDH in tobacco (Nicotiana tabacum) results in decreased sensitivity to Al and H + stress due to less production of formate in the transgenic tobacco lines under Al and H + stresses. Moreover, NtMATE and NtALS3 expression showed no changes versus wild type in these over-expression lines, suggesting that herein known Al-resistant mechanisms are not involved. Thus, the increased Al tolerance of VuFDH over-expression lines is likely attributable to their decreased Al-induced formate production. Taken together, our findings advance understanding of higher plant Al toxicity mechanisms, and suggest a possible new route toward the improvement of plant performance in acidic soils, where Al toxicity and H + stress coexist.

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“…Since the identification of transcription factors for Al tolerance in Arabidopsis (STOP1) and in rice (ART1) (Iuchi et al ., ; Yamaji et al ., ), a number of their homologs (ART1/STOP1‐like proteins) have been reported in different plant species, such as HlART1 in Yorkshire fog ( Holcus lanatus ) (Chen et al ., ), TaSTOP1 in wheat (Garcia‐Oliveira et al ., ), NtSTOP1 in tobacco ( N. tabacum ), PnSTOP1 in black poplar ( Populus nigra ), CsSTOP1 in tea ( Camellia sinensis ) (Ohyama et al ., ), STOP2 in Arabidopsis (Kobayashi et al ., ), EguSTOP1 in eucalyptus ( Eucalyptus ) (Sawaki et al ., ), VuSTOP1 in rice bean ( Vigna umbellata ) (Fan et al ., ), CcSTOP1 in pigeon pea ( Cajanus cajan ) (Daspute et al ., ), and SbSTOP1 in sweet sorghum ( Sorghum bicolor ) (Huang et al ., ). STOP1 in Arabidopsis and NtSTOP1 in tobacco regulate both Al tolerance and proton‐toxicity tolerance (Ohyama et al ., ). However, ART1 in rice only regulates Al tolerance (Yamaji et al ., ), while some of them, such as VnSTOP1 in rice bean and STOP2 in Arabidopsis, only regulate proton tolerance (Kobayashi et al ., ; Fan et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Functional characterization of an aluminum (Al)‐inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice

et al. 2018

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High aluminum (Al) tolerance in rice (Oryza sativa) is controlled by a Cys2His2-type zinc finger transcription factor ART1 (Al resistance transcription factor 1). There are five close homologs of ART1 in the rice genome, but the role of these homologs is unknown. We functionally characterized one of the ART1 homologs, ART2, in terms of tissue and spatial expression, subcellular localization, transcriptional activation activity, and phenotypic analysis of the knockout lines. ART2 was localized to the nucleus and showed a transcriptional activation potential in yeast. ART2 was mainly expressed in the roots, but the expression level was much lower than that of ART1. The ART2 expression was rapidly induced by Al in the roots of the wild-type rice, but not in art1 mutant. Knockout of ART2 resulted in increased sensitivity to Al toxicity, but did not alter sensitivity to different pH values. Expression profile analysis by RNA-sequencing showed that ART2 was not involved in activation of genes regulated by ART1; however, four genes seems to be regulated by ART2, which are implicated in Al tolerance. These results indicate that ART1 and ART2 regulate different pathways leading to Al tolerance, and ATR2 plays a supplementary role in Al tolerance in rice.

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Characterization of AtSTOP1 Orthologous Genes in Tobacco and Other Plant Species

Cited by 81 publications

References 42 publications

An Oxalyl-CoA Synthetase Is Involved in Oxalate Degradation and Aluminum Tolerance

An Oxalyl-CoA Synthetase Is Involved in Oxalate Degradation and Aluminum Tolerance

A Formate Dehydrogenase Confers Tolerance to Aluminum and Low pH

Functional characterization of an aluminum (Al)‐inducible transcription factor, ART2, revealed a different pathway for Al tolerance in rice

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